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Effect of dietary supplementation of garlic powder and phenyl acetic acid on productive performance, blood haematology, immunity and antioxidant status of broiler chickens

  • Ismail, I.E. (Poultry Department, Faculty of Agriculture, Zagazig University) ;
  • Alagawany, M. (Poultry Department, Faculty of Agriculture, Zagazig University) ;
  • Taha, A.E. (Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University) ;
  • Puvaca, N. (Department of Engineering Management in Biotechnology, Faculty of Economics and Engineering Management in Novi Sad, University Business Academy in Novi Sad) ;
  • Laudadio, V. (Department of DETO, Section of Veterinary Science and Animal Production, University of Bari "Aldo Moro") ;
  • Tufarelli, V. (Department of DETO, Section of Veterinary Science and Animal Production, University of Bari "Aldo Moro")
  • Received : 2020.03.03
  • Accepted : 2020.04.14
  • Published : 2021.03.01

Abstract

Objective: The effect of garlic powder (GP) and phenyl acetic (PA) acid throughout the fattening period of broiler chickens on performance, blood parameters, immune, and antioxidant parameters as well as carcass traits was evaluated. Methods: A total of 210 day-old Cobb broiler chicks were randomly distributed into seven dietary treatments having five replications with six chicks per replicate. The first group (control) fed a basal diet without supplements, whereas the 2nd, 3rd, and 4th group were fed basal diet plus 0.25, 0.50, and 0.75 g GP/kg diet, respectively and the group 5th, 6th, and 7th were fed on the basal diet plus 0.25, 0.50, and 0.75 g PA/kg diet. Results: Broiler body weight and gain at 21 and 42 days were increased (p<0.05) with diets supplemented with GP and PA. Red blood cells and hemoglobin were improved in chickens fed diets enriched with GP. Broiler chickens received diets containing either GP or PA recorded the higher values (p<0.05) of total protein, globulin, high-density lipoprotein, immunoglobulin M (IgM), and IgG, superoxide dismutase and total antioxidant capacity; while, blood total cholesterol, low-density lipoprotein, aspartate-aminotransferase, and malondialdehyde were lowered (p<0.05) compared to control-diet. Liver and immune-related organs weight were improved (p<0.05) in broilers fed diet supplemented with GP and PA. Conclusion: Feeding of GP or PA in diet had positive effects on performance traits and immunological, antioxidant and physiological status of broilers. Thus, the use of tested feed additives as an eco-friendly alternative to antibiotics produced a positive effect on animal health.

Keywords

References

  1. Saleha AA, Myaing TT, Ganapathy KK, Zulkifli I, Raha R, Arifah K. Possible effect of antibiotic-supplemented feed and environment on the occurrence of multiple antibiotic resistant Escherichia coli in chickens. Int J Poult Sci 2009;8: 28-31. https://doi.org/10.3923/ijps.2009.28.31
  2. Khoobani M, Hasheminezhad SH, Javandel F, et al. Effects of dietary chicory (Chicorium intybus L.) and probiotic blend as natural feed additives on performance traits, blood biochemistry, and gut microbiota of broiler chickens. Antibiotics 2020;9:5. https://doi.org/10.3390/antibiotics9010005
  3. Dhama K, Latheef SK, Mani S, et al. Multiple beneficial applications and modes of action of herbs in poultry health and production-a review. Int J Pharmacol 2015;11:152-76. https://doi.org/10.3923/ijp.2015.152.176
  4. Puvaca N, Kostadinovic L, Popovic S, et al. Proximate composition, cholesterol concentration and lipid oxidation of meat from chickens fed dietary spice addition (Allium sativum, Piper nigrum, Capsicum annuum). Anim Prod Sci 2016;56: 1920-7. https://doi.org/10.1071/AN15115
  5. Javandel F, Navidshad B, Seifdavati J, Pourrahimi GH, Bani-yaghoub S. The favorite dosage of garlic meal as a feed additive in broiler chickens ratios. Pak J Biol Sci 2008;11:1746-9. https://doi.org/10.3923/pjbs.2008.1746.1749
  6. Khan RU, Nikousefat Z, Tufarelli V, Naz S, Javdani M, Laudadio V. Garlic (Allium sativum) supplementation in poultry diets: effect on production and physiology. Worlds Poult Sci J 2012; 68:417-24. https://doi.org/10.1017/S0043933912000530
  7. Ziarlarimi A, Irani M, Gharahveysi S, Rahmani Z. Investigation of antibacterial effects of garlic (Allium sativum), mint (Menthe spp.) and onion (Allium cepa) herbal extracts on Escherichia coli isolated from broiler chickens. Afr J Biotechnol 2011;10:10320-2. https://doi.org/10.5897/AJB10.2513
  8. Teshika JD, Zakariyyah AM, Zaynab T, et al. Traditional and modern uses of onion bulb (Allium cepa L.): a systematic review. Crit Rev Food Sci Nutr 2019;59(Suppl 1):S39-70. https://doi.org/10.1080/10408398.2018.1499074
  9. Kim YJ, Jin SK, Yang HS. Effect of dietary garlic bulb and husk on the physicochemical properties of chicken meat. Poult Sci 2009;88:398-405. https://doi.org/10.3382/ps.200800179
  10. Stanacev V, Glamocic D, Milosevic N, Puvaca N, Stanacev V, Plavsa N. Effect of garlic (Allium sativum L.) in fattening chicks nutrition. Afr J Agric Res 2011;6:943-8. https://doi.org/10.5897/AJAR10.908
  11. Ur Rahman S, Khan S, Chand N, Sadique U, Khan RU. In vivo effects of Allium cepa L. on the selected gut microflora and intestinal histomorphology in broiler. Acta Histochem 2017; 119:446-50. https://doi.org/10.1016/j.acthis.2017.04.004
  12. Adams C. Poultry and dietary acids. Feed Int 1999;20:13702.
  13. Dhama K, Tiwari R, Khan RU, et al. Growth promoters and novel feed additives improving poultry production and health, bioactive principles and beneficial applications: the trends and advances-a review. Int J Pharmacol 2014;10:129-59. https://doi.org/10.3923/ijp.2014.129.159
  14. Cooksley J. Acetic acid supplementation reduces the effects of heat stress in poultry. In: Anitox Solution for Safer Feed and Food USA. 2011.
  15. Viveros A, Brenes A, Arija I, Centeno C. Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poult Sci 2002;81:1172-83. https://doi.org/10.1093/ps/81.8.1172
  16. NRC. Nutrient requirements of poultry. 9th rev ed. Washington, DC, USA: National Academies Press; 1994.
  17. Ebrahimi A, Qotbi AAA, Seidavi A, Laudadio V, Tufarelli V. Effect of different levels of dried sweet orange (Citrus sinensis) peel on broiler chickens growth performance Abbas. Arch Anim Breed 2013;56:11-7. https://doi.org/10.7482/0003-943856-002
  18. Lamberg SL, Rothstein R. Laboratory manual of hematology and urinalysis. West Port, CT, USA: Avid Publishing Company, Inc.; 1978.
  19. Campbell TW. Avian hematology and cytology. Ames, IA, USA: Iowa State University Press; 1988. pp. 3-17.
  20. Suriya R, Zulkifli I, Alimon AR. The effect of dietary inclusion of herbs as growth promoter in broiler chickens. J Anim Vet Adv 2012;11:346-50. http://dx.doi.org/10.3923/javaa.2012.346.350
  21. Nourmohammadi R, Khosravinia H. Acidic stress caused by dietary administration of citric acid in broiler chickens. Arch Anim Breed 2015;58:309-15. https://doi.org/10.5194/aab-58309-2015
  22. Ross ZM, O'Gara EA, Hill DJ, Sleightholme HV, Maslin DJ. Antimicrobial properties of garlic oil against human enteric bacteria: evaluation of methodologies and comparisons with garlic oil sulfides and garlic powder. Appl Environ Microbiol 2001;67:475-80. https://doi.org/10.1128/AEM.67.1.475-480.2001
  23. Dibner JJ, Buttin P. Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism. J Appl Poult Res 2002;11:453-63. https://doi.org/10.1093/japr/11.4.453
  24. Ashayerizadeh O, Dastar B, Shargh MS, Ashayerizadeh A, Rahmatnejad E, Hossaini SMR. Use of garlic (Allium sativum), black cumin seeds (Nigella sativa L.) and wild mint (Mentha longifolia) in broiler chickens diets. J Anim Vet Adv 2009;8: 1860-3.
  25. Raeesi M, Hoseini-Aliabad SA, Roofchaee A, Zare Shahneh A, Pirali S. Effect of periodically use of garlic (Allium sativum) powder on performance and carcass characteristics in broiler chickens. World Acad Sci Eng Technol 2010;4:1388-94.
  26. Sohail R, Saeed M, Chao S, et al. Comparative effect of different organic acids (benzoic, acetic and formic) on growth performance, immune response and carcass traits of broilers. J Anim Prod Adv 2015;5:757-64. https://doi.org/10.5455/japa.20150924120241
  27. Denli M, Okan F, Celik K. Effect of dietary probiotic, organic acid and antibiotic supplementation to diets on broiler performance and carcass yield. Pak J Nutr 2003;2:89-91. https://doi.org/10.3923/pjn.2003.89.91
  28. Hanieh H, Narabara K, Piao M, Gerile C, ABE A, Kondo Y. Modulatory effects of two levels of dietary Alliums on immune response and certain immunological variables, following immunization, in White Leghorn chickens. Anim Sci J 2010; 81:673-80. https://doi.org/10.1111/j.1740-0929.2010.00798.x
  29. Askar AA. Some physiological and immunological effects of phenyllactic acid in broiler chicks. Egypt J Anim Prod 2012; 49:87-93. https://doi.org/10.21608/ejap.2012.94355
  30. Rehman Z, Haq A, Akram N, et al. Growth performance, intestinal histomorphology, blood hematology and serum metabolites of broilers chickens fed diet supplemented with graded levels of acetic acid. Int J Pharmacol 2016;12:874-83. http://dx.doi.org/10.3923/ijp.2016.874.883
  31. Wang JP, Yoo JS, Lee JH, et al. Effects of phenyllactic acid on production performance, egg quality parameters, and blood characteristics in laying hens. J Appl Poult Res 2009;18:2039. https://doi.org/10.3382/japr.2008-00071
  32. Haque MN, Islam KMS, Akbar MA, et al. Effect of dietary citric acid, flavomycin and their combination on the performance, tibia ash and immune status of broiler. Can J Anim Sci 2010;90:57-63. https://doi.org/10.4141/CJAS09048
  33. Elagib HAA, El-Amin WIA, Elamin KM, Malik HEE. Effect of dietary garlic (Allium sativum) supplementation as feed additive on broiler performance and blood profile. J Anim Sci Adv 2013;3:58-64. https://doi.org/10.5455/jasa.20130219104029
  34. Rehman ZU, Munir MT. Effect of garlic on the health and performance of broilers. Veterinaria 2015;3:32-9.
  35. Dorhoi A, Dobrean V, Zahan M, Virag P. Modulatory effects of several herbal extracts on avian peripheral blood cell immune responses. Phytother Res 2006;20:352-8. https://doi.org/10.1002/ptr.1859
  36. Rahimi S, Teymouri Zadeh Z, Karimi Torshizi MA, Omidbaigi R, Rokni H. Effect of the three herbal extracts on growth performance, immune system, blood factors and intestinal selected bacterial population in broiler chickens. J Agric Sci Technol 2011;13:527-39.
  37. Issa KJ, Omar JMA. Effect of garlic powder on performance and lipid profile of broilers. Open J Anim Sci 2012;2:62-8. https://doi.org/10.4236/ojas.2012.22010
  38. Mahmoud KZ, Gharaibeh SM, Zakaria HA, Qatramiz AM. Garlic (Allium sativum) supplementation: influence on egg production, quality, and yolk cholesterol level in layer hens. Asian-Australas J Anim Sci 2010;23:1503-9. https://doi.org/10.5713/ajas.2010.10124
  39. Rahmani HR, Speer W. Natural additives influence the performance and humoral immunity of broilers. Int J Poult Sci 2005;4:713-7. https://doi.org/10.3923/ijps.2005.713.717
  40. Salgado-Transito L, Del Rio-Garcia JC, Arjona-Roman JL, Moreno-Martinez E, Mendez-Albores A. Effect of citric acid supplemented diets on aflatoxin degradation, growth performance and serum parameters in broiler chickens. Arch Med Vet 2011;43:215-22. https://doi.org/10.4067/S0301-732X2011000300003
  41. Hossain MM, Lee SI, Kim IH. Effect of dietary Korean aged garlic extract by Leukonostoc citreum SK2556 on production, hematological status, meat quality, relative organ weight, targeted Escherichia coli colony and excreta gas emission in broilers. Anim Feed Sci Technol 2014;198:333-40. https://doi.org/10.1016/j.anifeedsci.2014.09.015
  42. Pourali M, Kermanshahi H, Golian A, Razmi GR, Soukhtanloo M. Antioxidant and anticoccidial effects of garlic powder and sulfur amino acids on Eimeria-infected and uninfected broiler chickens. Iran J Vet Res 2014;15:227-32. https://doi.org/10.22099/ijvr.2014.2531
  43. Nasiroleslami M, Torki M, Saki AA, Abdolmohammadi AR. Effects of dietary guanidinoacetic acid and betaine supplementation on performance, blood biochemical parameters and antioxidant status of broilers subjected to cold stress. J Appl Anim Res 2018;46:1016-22. https://doi.org/10.1080/09712119.2018.1450751
  44. Alagawany M, Ashour E, Reda FM. Effect of dietary supplementation of garlic (Allium sativum) and turmeric (Curcuma longa) on growth performance, carcass traits, blood profile and oxidative status in growing rabbits. Ann Anim Sci 2016; 16:489-505. https://doi.org/10.1515/aoas-2015-0079

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