The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa

  • Li, Guoliang (State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) ;
  • Zhang, Shifan (State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) ;
  • Li, Fei (State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) ;
  • Zhang, Hui (State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) ;
  • Zhang, Shujiang (State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) ;
  • Zhao, Jianjun (State Key Laboratory of North China Crop Improvement and Regulation, Department of Horticulture, Hebei Agricultural University) ;
  • Sun, Rifei (State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences)
  • 투고 : 2020.07.17
  • 심사 : 2020.12.31
  • 발행 : 2021.02.01
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초록

Plants protect against viruses through passive and active resistance mechanisms, and in most cases characterized thus far, natural recessive resistance to potyviruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimolecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and translation initiation factors to reveal the working of key amino acids.

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참고문헌

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