Advances in nano research

  • 제10권2호
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  • Pages.139-150
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  • 2021
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  • 2287-237X(pISSN)
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  • 2287-2388(eISSN)
테크노프레스 (Techno-Press)
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DOI QR코드

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Antioxidative and antiproliferative effects of propolis-reduced silver nanoparticles

  • Tan, Gamze (Department of Biology, Faculty of Science and Letters, Aksaray University) ;
  • Ilk, Sedef (Department of Immunology, Faculty of Medicine, Nigde O mer Halisdemir University) ;
  • Foto, Fatma Z. (Department of Biochemistry, Faculty of Science, Selcuk University) ;
  • Foto, Egemen (Department of Biotechnology, Faculty of Science, Necmettin Erbakan University) ;
  • Saglam, Necdet (Department of Nanotechnology and Nanomedicine, Institute of Science and Engineering, Hacettepe University)
  • 투고 : 2019.12.22
  • 심사 : 2020.12.08
  • 발행 : 2021.02.25
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, phytochemicals present in Propolis Extract (PE) were employed as reducing and stabilizing reagents to synthesize silver nanoparticles. Three propolis-reduced silver nanoparticles (P-AgNPs1-3) were synthesized using increasing amounts of PE. P-AgNPs were treated with different cancer cells-lung (A549), cervix (HeLa) and colon (WiDr) - for 24, 48 and 72 h to evaluate their anti-proliferative activities. A non-cancerous cell type (L929) was also used to test whether suppressive effects of P-AgNPs on cancer cell proliferation were due to a general cytotoxic effect. The characterization results showed that the bioactive contents in propolis successfully induced particle formation. As the amount of PE increased, the particle size decreased; however, the size distribution range expanded. The antioxidant capacity of the particles increased with increased propolis amounts. P-AgNP1 exhibited almost equal inhibitory effects across all cancer cell types; however, P-AgNP2 was more effective on HeLa cells. P-AgNPs3 showed greater inhibitory effects in almost all cancer cells compared to other NPs and pure propolis. Consequently, the biological effects of P-AgNPs were highly dependent on PE amount, NP concentration, and cell type. These results suggest that AgNPs synthesized utilizing propolis phytochemicals might serve as anti-cancer agents, providing greater efficacy against cancer cells.

키워드

과제정보

The authors would like to thank Ph.D. candidate Ezgi Emul for her technical guidance. There are no conflicts of interest.

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