Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Lagerstroemia ovalifolia Exerts Anti-Inflammatory Effects in Mice of LPS-Induced ALI via Downregulating of MAPK and NF-κB Activation

  • Min, Jae-Hong (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Seong-Man (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, JI-Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Nam Hoon (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Goo, Soo Hyeon (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ngatinem, Ngatinem (Starch Technology Center, Agency for the Assessment and Application Technology) ;
  • Ningsih, Sri (Center for Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology) ;
  • Paik, Jin-Hyub (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Sangho (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Sei-Ryang (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Han, Sang-Bae (College of Pharmacy, Chungbuk National University) ;
  • Ahn, Kyung-Seop (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jae-Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2021.07.12
  • Accepted : 2021.09.01
  • Published : 2021.11.28
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Abstract

Lagerstroemia ovalifolia Teijsm. & Binn. (LO) (crape myrtle) has reportedly been used as traditional herbal medicine (THM) in Java, Indonesia. Our previous study revealed that the LO leaf extract (LOLE) exerted anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Based on this finding, the current study aimed to evaluate the protective effects of LOLE in a mouse model of LPS-induced acute lung injury (ALI). The results showed that treatment with LPS enhanced the inflammatory cell influx into the lungs and increased the number of macrophages and the secretion of the inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) of mice. However, these effects were notably abrogated with LOLE pretreatment. Furthermore, the increase of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1) expression in the lung tissues of mice with ALI was also reversed by LOLE. In addition, LOLE significantly suppressed the LPS-induced activation of the MAPK/NF-κB signaling pathway and led to heme oxygenase-1 (HO-1) induction in the lungs. Additionally, in vitro experiments showed that LOLE enhanced the expression of HO-1 in RAW264.7 macrophages. The aforementioned findings collectively indicate that LOLE exerts an ameliorative effect on inflammatory response in the airway of ALI mice.

Keywords

Acknowledgement

This research was supported by grants from the Korea Research Institute of Bioscience and Biotechnology Research Initiative Program (KRIBB) (Grant No. KGM5522113 and KGS1402113) of the Republic of Korea.

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