Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Direct and Indirect Reduction of Cr(VI) by Fermentative Fe(III)-Reducing Cellulomonas sp. Strain Cellu-2a

  • Khanal, Anamika (Department of Bioenvironmental Chemistry, Jeonbuk National University) ;
  • Hur, Hor-Gil (School of Environmental and Earth Science, Gwangju Institute of Science and Technology) ;
  • Fredrickson, James K. (Pacific Northwest National Laboratory) ;
  • Lee, Ji-Hoon (Department of Bioenvironmental Chemistry, Jeonbuk National University)
  • Received : 2021.07.20
  • Accepted : 2021.09.01
  • Published : 2021.11.28
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Abstract

Hexavalent chromium (Cr(VI)) is recognized to be carcinogenic and toxic and registered as a contaminant in many drinking water regulations. It occurs naturally and is also produced by industrial processes. The reduction of Cr(VI) to Cr(III) has been a central topic for chromium remediation since Cr(III) is less toxic and less mobile. In this study, fermentative Fe(III)-reducing bacterial strains (Cellu-2a, Cellu-5a, and Cellu-5b) were isolated from a groundwater sample and were phylogenetically related to species of Cellulomonas by 16S rRNA gene analysis. One selected strain, Cellu-2a showed its capacity of reduction of both soluble iron (ferric citrate) and solid iron (hydrous ferric oxide, HFO), as well as aqueous Cr(VI). The strain Cellu-2a was able to reduce 15 μM Cr(VI) directly with glucose or sucrose as a sole carbon source under the anaerobic condition and indirectly with one of the substrates and HFO in the same incubations. The heterogeneous reduction of Cr(VI) by the surface-associated reduced iron from HFO by Cellu-2a likely assisted the Cr(VI) reduction. Fermentative features such as large-scale cell growth may impose advantages on the application of bacterial Cr(VI) reduction over anaerobic respiratory reduction.

Keywords

Acknowledgement

This research was funded by "Basic Science Research Program" through the National Research Foundation of Korea (NRF) (NRF-2016R1D1A3B01012231). We would also like to express our gratitude to the editors of the Writing Center at Jeonbuk National University for their skilled English-language assistance.

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