Membrane Journal (멤브레인)

The Membrane Society of Korea (한국막학회)
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Applications of Enzyme Immobilized Membranes: A Review

효소 고정화막의 응용에 대한 총설

  • Ryu, Junghyun (Life Science and Biotechnology Department (LSBT), Underwood Division (UD), Underwood International College, Yonsei University) ;
  • Patel, Rajkumar (Energy and Environmental Science and Engineering (EESE), Integrated Science and Engineering Division (ISED), Underwood International College, Yonsei University) ;
  • Kim, Jong Hak (Department of Chemical and Biomolecular Engineering, Yonsei University)
  • 유정현 (연세대학교 언더우드학부 생명과학공학과) ;
  • 라즈쿠마 파텔 (연세대학교 언더우드학부 융합과학공학부 에너지환경융합전공) ;
  • 김종학 (연세대학교 화공생명공학과)
  • Received : 2021.12.18
  • Accepted : 2021.12.27
  • Published : 2021.12.31
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Abstract

Enzymes are important class of catalyst for biotransformation. Stability and reusability of enzymes during the catalysis process is a key issue. Activity of enzyme can be enhanced by its immobilization on a suitable substrate by creation of specific microenvironment. A variety of membranes has been used as substrate due to the biocompatibility and simpler method to tune hydrophilicity/hydrophobicity property of the membrane surface. In this review, polymer membranes including cellulose, polyacrylonitrile (PAN), polydimethylsiloxane (PDMS), polyvinylidene fluoride (PVDF), polyethersulfone (PES) are introduced and discussed in detail. Biodegradation of organic contaminants by immobilized enzyme is an environmental friendly process to reduce the contamination of environment in pharmaceutical company and textile industries. The controlled hydrolysis of oil can be performed in enzyme immobilized membrane bioreactor (EMBR), resulting in reducing carbon emission and reduced environmental pollution. Bioethanol and biodiesel are considered alternative fossil fuels that can be prepared in EMBR.

생체 내 변화에서 효소는 중요한 촉매이다. 효소의 안정성과 재사용성은 촉매 과정에서 중요한 요소이다. 적합한 기질에 효소 고정화는 특정 미세환경의 조성을 통해 효소 활동성을 높인다. 다양한 종류의 분리막이 각각의 생체적합성과 막 표면의 친수성/소수성 조절 용이도에 따라 기질로 사용되었다. 본 논문에서는 셀룰로스, 폴리아크릴로니트릴(PAN), 폴리디메틸실록산(PDMS), 폴리비닐리덴플루오라이드(PVDF), 폴리에테르설폰(PES) 고분자 분리막이 소개되고 토의되었다. 고정화 효소를 이용한 유기오염물의 생물적 분해는 제약 회사 및 섬유 회사 등에서 발생하는 오염물질을 친환경적으로 감소할 수 있는 방법이다. 효소 고정화 생물반응기(EMBR)로 기름의 가수분해를 제어할 수 있고 이를 통해 탄소 배출량 감소 및 환경오염을 줄일 수 있다. EMBR로 만들 수 있는 바이오에탄올과 바이오디젤은 화석 연료의 대체제이다.

Keywords

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