한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제66권4호
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  • Pages.326-338
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  • 2021
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
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UPLC 분석을 이용한 맥주보리 품종의 호데인 단백질 분석 및 품종 판별

Discrimination and Hordein Polypeptide Patterns of Malting Barley Varieties Using UPLC

  • 윤영미 (농촌진흥청 국립식량과학원) ;
  • 김양길 (농촌진흥청 국립식량과학원) ;
  • 강천식 (농촌진흥청 국립식량과학원) ;
  • 박진천 (농촌진흥청 국립식량과학원) ;
  • 박태일 (농촌진흥청 국립식량과학원)
  • 투고 : 2021.10.08
  • 심사 : 2021.11.15
  • 발행 : 2021.12.01
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초록

맥주보리 품종의 C-hordeins 분석을 위해 70% EtOH와 55% IPA가 추출용매로 이용되었으며, B-hordeins의 추출은 알코올성 용매에 1% DTT를 추가하여 수행되었다. D-hordeins은 보리 호데인 단백질 중 가장 분자량이 큰 단백질로 약산 혹은 약염기에서 용해가 이루어진다고 알려져 있으며, 본 연구에서는 pH 8.0의 약염기 용매가 추출용매로 이용되었다. 알코올성 용매 종류에 따른 C-hordeins의 구성과 양의 차이는 확인되지 않았으며 1% DTT가 첨가된 알코올성 용매에 B-hordeins과 C-hordeins이 모두 추출됨이 확인되었다. D-hordeins 추출 조건에서는 B-, C-, D-hordeins 이 모두 분석되었지만 B-hordeins의 머무름 시간은 1% DTT가 함유된 알코올 추출과 차이가 있었다. 국내 육성된 맥주보리 26품종들의 C-hordeins 분석을 통해 머무름 시간 16분에서 18분 사이의 피크 유사성을 가지고 7개의 그룹으로 분류할 수 있었다. 또한 맥주보리 육성 품종 중 사천6호와 두산29호, 진광보리와 제주보리, 호품과 다품보리를 제외한 20개 품종들은 마이너 피크들의 분석을 통해 품종의 구분이 가능하였다.

Ultra-performance liquid chromatography (UPLC) was used to assess the hordein protein fraction of malting barley. C-hordeins (barley prolamins) were extracted with 70% ethanol (EtOH) and 55% isopropyl alcohol (IPA, 2-propanol), and B-hordeins were extracted with the same alcohols in 1.0% dithiothreitol (DTT). High molecular weight (HMW) prolamins (D-hordeins) were extracted with 50% IPA with 1M Tris-HCl (pH 8.0). The same protein patterns were observed in both the experimental extraction solutions (EtOH and IPA). However, the patterns of hordein, extracted with EtOH and IPA containing 1.0% DTT, differed slightly. C- and B-hordeins extracted from those solutions were analyzed. Twenty-six malting barley varieties developed in Korea were analyzed using UPLC. The varieties were divided into seven groups according to hordein patterns of retention time 16 min to 18 min, and 20 varieties showed unique patterns.

키워드

과제정보

본 논문은 농촌진흥청 농업과학기술 연구개발 사업(과제명: 국내 육성 맥주보리의 품종판별 분자마커개발: PJ014209012021)의 지원에 의해 이루어진 것이며, 이의 지원에 감사드립니다.

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