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Improvement of Orchardgrass (Dactylis glomerata L.) Silage Quality by Lactic Acid Bacteria

  • Ilavenil, Soundharrajan (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Muthusamy, Karnan (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Jeong Sung (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Bae Hun (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Hyung Soo (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Ki Choon (Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2021.09.02
  • Accepted : 2021.12.16
  • Published : 2021.12.31

Abstract

In the current study, lactic lactic acid bacteria (LAB) Lactobacillus plantarum and Pediococcus pentosaceus were used as a mixed additive for the production of Orchardgrass silage by ensiled method and nutritional change fermentation ability and microbial content of experimental silages. The addition of LAB to Orchardgrass during ensiling process rapidly reduced the pH of the silages than the non-inoculated silages. In addition, the lactic and acetic acid content of silage was increased by LAB strains than the non-inoculated silages whereas butyric acid content was reduced in silage treated with LAB. A microbiological study revealed that higher LAB but lower yeast counts were observed in inoculated silages compared to non-inoculated silage. Overall data suggested that the addition of LAB stains could have ability to induce the fermentation process and improve the silage quality via increasing lactic acid and decreasing undesirable microbes.

Keywords

Acknowledgement

Cooperative Research Program for Agriculture Science and Technology Development supported funds for this research work (Project No. PJ01499606). The project titled "technique development for manufacture of high-quality legume silage"sponsored by RDA, Korea. This study was also supported by the Postdoctoral Fellowship Program of the National Institute of Animal Science funded by RDA, Korea.

References

  1. AOAC. 2000. Official methods of analysis (17th ed.). Gaithersburg, MD, USA.
  2. Arasu, M.V., Jung, M.W., Kim, D.H., Ilavenil, S., Jane, M., Park, H.S., Al-Dhabi, N.A., Jeon, B.T. and Choi, K.C. 2014. Enhancing nutritional quality of silage by fermentation with Lactobacillus plantarum. Indian Journal of Microbiology. 54:396-402. https://doi.org/10.1007/s12088-014-0473-9
  3. Bourquin, L.D., Titgemeyer, E.C., Merchen, N.R. and Fahey Jr, G.C. 1994. Forage level and particle size effects on orchardgrass digestion by steers: I. Site and extent of organic matter, nitrogen, and cell wall digestion. Journal of Animal Science. 72:746-758. https://doi.org/10.2527/1994.723746x
  4. Burns, P., Borgo, M.F., Binetti, A., Puntillo, M., Bergamini, C., Paez, R., Mazzoni, R., Reinheimer, J. and Vinderola, G. 2018. Isolation, characterization and performance of autochthonous spray dried lactic acid bacteria in maize micro and bucket-silos. Frontiers in Microbiology. 9:2861. https://doi.org/10.3389/fmicb.2018.02861
  5. Butkute, B., Lemeziene, N., Kanapeckas, J., Navickas, K., Dabkevicius, Z. and Venslauskas, K. 2014. Cocksfoot, tall fescue and reed canary grass: Dry matter yield, chemical composition and biomass convertibility to methane. Biomass and Bioenergy. 66:1-11. https://doi.org/10.1016/j.biombioe.2014.03.014
  6. Danner, H., Holzer, M., Mayrhuber, E. and Braun, R. 2003. Acetic acid increases stability of silage under aerobic conditions. Applied and Environmental Microbiology. 69:562-567. https://doi.org/10.1128/AEM.69.1.562-567.2003
  7. Davies, D.R., Theodorou, M.K., Kingston-Smith, A.H. and Merry, R.J. 2005. Advances in silage quality. Pages 121-133 in the 21st century. 14th International Silage Conference. Proc. Belfast, Northern Ireland.
  8. Di Cagno, R., Coda, R., De Angelis, M. and Gobbetti, M. 2013. Exploitation of vegetables and fruits through lactic acid fermentation. Food Microbiolgy. 33:1-10. https://doi.org/10.1016/j.fm.2012.09.003
  9. Guan, H., Ke, W., Yan, Y., Shuai, Y., Li, X., Ran, Q., Yang, Z., Wang, X., Cai, Y. and Zhang, X. 2020. Screening of natural lactic acid bacteria with potential effect on silage fermentation, aerobic stability and aflatoxin B1 in hot and humid area. Journal of Applied Microbiology. 12:1301-1311. https://doi.org/10.1111/jam.14570
  10. Guo, L., Lu, Y., Li, P., Chen, L., Gou, W. and Zhang, C. 2021. Effects of delayed harvest and additives on fermentation quality and bacterial community of corn stalk silage. Frontiers in Microbiology. 12:687481. https://doi.org/10.3389/fmicb.2021.687481
  11. Jalc, D., Laukova, A., Pogany Simonova, M., Varadyova, Z. and Homolka, P. 2009. The use of bacterial inoculants for grass silage: Their effects on nutrient composition and fermentation parameters in grass silages. Czech Journal of Animal Science. 54:83-90. https://doi.org/10.17221/1665-CJAS
  12. Kristensen, N.B., Storm, A., Raun, B.M.L., Rojen, B.A. and Harmon, D.L. 2007. Metabolism of silage alcohols in lactating dairy cows. Journal of Dairy Science. 90:1364-1377. https://doi.org/10.3168/jds.S0022-0302(07)71623-5
  13. Kung Jr, L., Shaver, R.D., Grant, R.J. and Schmidt, R.J. 2018. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science. 101:4020-4033. https://doi.org/10.3168/jds.2017-13909
  14. Kung, L. and Shaver, R. 2001. Interpretation and use of silage fermentation analysis reports, focus on forage. University of Wisconsin Extension. 3:1-5.
  15. Kung, L., Shaver, R.D., Grant, R.J. and Schmidt, R.J. 2018. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science. 101:4020-4033. https://doi.org/10.3168/jds.S0022-0302(07)71623-5
  16. Lahtinen, S., Ouwehand, A., Salminen, S. and Wright, A. 2012. Lactic acid bacteria: Microbiological and functional aspects (4th ed.). CRC Press. p. 798.
  17. McDonald, P., Henderson, A.R., Heron, S.J., McDonald, P., Henderson, A.R. and Heron, S.J. 1991. The biochemistry of silage (2nd ed.). Cambridge University Press. p. 340.
  18. Muck, R.E. 2010. Silage microbiology and its control through additives. Revista Brasileira de Zootecnia. 39: 183-191. https://doi.org/10.1590/S1516-35982010001300021
  19. Muck, R.E. and Kung, L. 1997. Effects of silage additives on ensiling. NRAES-99, Northeast Regional Agricultural Engineering Service, Ithaca, N. Y. pp. 187-199.
  20. Muck, R.E., Nadeau, E.M.G., McAllister, T.A., Contreras-Govea, F.E., Santos, M.C. and Kung, L. 2018. Silage review: Recent advances and future uses of silage additives. Journal of Dairy Science. 101:3980-4000. https://doi.org/10.3168/jds.2017-13839
  21. Murphy, R.P. 2010. A method for the extraction of plant samples and the determination of total soluble carbohydrates. Journal of the Science of Food and Agriculture. 2010:9. https://doi.org/10.1002/jsfa.2740091104
  22. Muthusamy, K., Soundharrajan, I., Srisesharam, S., Kim, D., Kuppusamy, P., Lee, K.D. and Choi, K.C. 2020. Probiotic characteristics and antifungal activity of Lactobacillus plantarum and its impact on fermentation of Italian ryegrass at low moisture. Applied Sciences. 10:417. https://doi.org/10.3390/app10010417
  23. Nascimento Agarussi, M.C., Gomes Pereira, O., Paula, R.A.D., Silva, V.P.D., Santos Roseira, J.P. and Fonseca E Silva, F. 2019. Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation. Scientific Reports. 9:8007-8007. https://doi.org/10.1038/s41598-019-44520-9
  24. Niderkorn, V., Martin, C., Rochette, Y., Julien, S. and Baumont, R. 2015. Associative effects between orchardgrass and red clover silages on voluntary intake and digestion in sheep: Evidence of a synergy on digestible dry matter intake. Journal of Animal Science. 93:4967-4976. https://doi.org/10.2527/jas.2015-9178
  25. Ogunade, I.M., Jiang, Y., Pech Cervantes, A.A., Kim, D.H., Oliveira, A.S., Vyas, D., Weinberg, Z.G., Jeong, K.C. and Adesogan, A.T. 2018. Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: Effects of Escherichia coli O157:H7 and silage additives. Journal of Dairy Science. 101:2048-2059. https://doi.org/10.3168/jds.2017-12876
  26. Oliveira, A.S., Weinberg, Z.G., Ogunade, I.M., Cervantes, A.A.P., Arriola, K.G., Jiang, Y., Kim, D., Li, X., Goncalves, M.C.M., Vyas, D. and Adesogan, A.T. 2017. Meta-analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation, aerobic stability, and the performance of dairy cows. Journal of Dairy Science. 100:4587-4603. https://doi.org/10.3168/jds.2016-11815
  27. Pahlow, G., Muck, R.E., Driehuis, F., Oude Elferink, S.J.W.H. and Spoelstra, S.F. 2003. Microbiology of ensiling. In: D.R. Buxton, R.E. Muck, J.H. Harrison (Eds.), Silage Science and Technology. American Society of Agronomy. pp. 31-93.
  28. Soundharrajan, I., Muthusamy, K., Han, O.K., Lee, H.J., Purushothaman, S., Kim, D. and Choi, K.C. 2020. Effects of microbial inoculants on the fermentation and preservation of triticale silages at high and low moisture levels. Applied Sciences. 10:7855. https://doi.org/10.3390/app10217855
  29. Turner, L.R., Donaghy, D.J., Lane, P.A. and Rawnsley, R.P. 2007. A comparison of the establishment, productivity, and feed quality of four cocksfoot (Dactylis glomerata L.) and four brome (Bromus spp.) cultivars, under leaf stage based defoliation management. Australian Journal of Agricultural Research. 58:900-906. https://doi.org/10.1071/AR06252
  30. Van Soest, P.J., Roberstson, J.B. and Lewis, B.A. 1991. Methods for dietary fibre, neutral detergent fibre and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Sciences. 74:3583. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  31. Xue, Z., Liu, N., Wang, Y., Yang, H., Wei, Y., Moriel, P., Palmer, E. and Zhang, Y. 2019. Combining orchardgrass and alfalfa: Effects of forage ratios on in vitro rumen degradation and fermentation characteristics of silage compared with hay. Animals. 10:59. https://doi.org/10.3390/ani10010059
  32. Zhao, G.Q., Wei, S.N., Li, Y.F., Jeong, E.C., Kim, H.J. and Kim, J.G. 2020. Comparison of forage quality, productivity and β-carotene content according to maturity of forage rye (Secale cereale L.). Journal of the Korean Society of Grassland and Forage Science. 40:123-130. https://doi.org/10.5333/kgfs.2020.40.3.123