한방재활의학과학회지 (Journal of Korean Medicine Rehabilitation)

  • 제31권1호
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  • Pages.95-108
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  • 2021
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  • 1229-1854(pISSN)
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  • 2288-4114(eISSN)
한방재활의학과학회 (The society of Korean Medicine Rehabilitation)
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경옥고가미방의 베타글루칸, 진세노사이드 함량, 2,2-diphenyl-1-picrylhydrazyl (DPPH) Free Radical 소거 활성 및 단회 투여 독성 연구

A Study on the β-glucan, Ginsenoside Content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) Free Radical Scavenging Activity and Single Dose Toxicity Assessment of Modified Kyungohkgo

  • 이유미 (동신대학교 한의과대학 경혈진단학교실) ;
  • 문양선 ((유)나우리) ;
  • 박희명 (건국대학교 수의과대학 수의내과학교실) ;
  • 김형석 (건국대학교 수의과대학 수의내과학교실) ;
  • 노웅빈 (건국대학교 수의과대학 수의내과학교실) ;
  • 나창수 (동신대학교 한의과대학 경혈진단학교실)
  • Lee, Yu-Mi (Department of Meridian & Acupoint.Diagnosis, College of Korean Medicine, Dongshin University) ;
  • Moon, Yang-Seon (Nawoori (Ltd)) ;
  • Park, Hee-Myeong (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Heyong-Seok (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Ro, Woong-Bin (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Na, Chang-Su (Department of Meridian & Acupoint.Diagnosis, College of Korean Medicine, Dongshin University)
  • 투고 : 2020.12.22
  • 심사 : 2020.12.30
  • 발행 : 2021.01.31
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초록

Objectives This study was conducted to investigate the beta-glucan, ginsenoside content, antioxidant activity and safety of modified Kyungohkgo added to Sparassis crispa and Hericium erinaceum. Methods The marker compounds contents, antioxidant activity and safety of modified Kyungohkgo were tested. The contents of beta-glucan and ginsenoside Rb1, Rg1, and Rg3 marker compounds were measured, the antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and a safety test was conducted via single dose toxicity assessment. Results Analyzing the contents of marker compounds showed 351.75 mg/g of beta-glucan, 0.0327 mg/g of ginsenoside Rb1 and 0.0802 mg/g of ginsenosai Rg3. In the DPPH free radical scavenging activity, the inhibition concentration 50% of modified Kyungohkgo was 0.2880%. The scavenging activity of modified Kyungohkgo was 5.49% activity at 0.05% concentration, 89.66% activity at 0.5% concentration, 94.68% activity at 1% concentration, and 96.06% activity at 5% concentration. In the single dose toxicity test of modified Kyungohkgo, a dose of 2,000 mg/kg B.W. was set at its highest capacity and observed after oral administration to female and male rats. No toxicological findings were recognized. It was observed that the resulting lethal dose can be set to 2,000 mg/kg B.W. or higher for both females and males. Conclusions The results of the experiment on modified Kyungohkgo showed that the marker compounds contents were beta-glucan and ginsenoside Rb1 and Rg3, that antioxidant activity was observed through the DPPH free radical scavenging activity, and safety was confirmed through the single dose toxicity assessment.

키워드

과제정보

This paper has been written with the support of Jeollannam-do('2019 R&D supporting program' operated by Jeonnam Technopark.

참고문헌

  1. Heo J. Dongeuibogam. Seoul:Namsandang. 1989:78.
  2. Kim MD. The literature study on the efficacy and manufacturing process of Gyeongoggo. J Oriental Medical Classics. 2011;24(2):51-64.
  3. Na CS, Shin W, Lee YM, Moon YS, Noh HK, Seo SH, Son HS. Effect of original Kyungokgo & Iksuyongjingo plus Sparassis crispa on antioxidant, immunity improvement and sensory evaluation. Kor J Herbol. 2016;31(4): 43-51. https://doi.org/10.6116/kjh.2016.31.4.43.
  4. Chan GC, Chan WK, Sze DM. The effects of α-glucan on human immune and cancer cells. J Hematol Oncol. 2009;2:25. https://doi.org/10.1186/1756-8722-2-25
  5. Ohno N, Miura NN, Nakajima M, Yadomae T. Antitumor 1,3-β-glucan from cultured fruit body of Sparassis crispa. Biol Pharm Bull. 2000;23:866-72. https://doi.org/10.1248/bpb.23.866
  6. Kim IK, Yun YC, Shin YC, Yoo J. Effect of Sparassis crispa extracts on immune cell activation and tumor growth inhibition. J Life Sci. 2013;23:984-8. https://doi.org/10.5352/JLS.2013.23.8.984
  7. Kim MS, Lee KT, Jeon SM, Ka KH. The quantities of methyl orsellinate and sparassol of Sparassis latifolia by host plants. Kor J Mycol. 2013;41:236-42. https://doi.org/10.4489/KJM.2013.41.4.236
  8. Choi WS, Shin PG, Yoo YB, Noh HJ, Kim GD. Anti-inflammatory effects of Sparassis crispa extracts. J Mushroom Sci Prod. 2013;11:46-51. https://doi.org/10.14480/JM.2013.11.1.046
  9. Kim MY, Seguin P, Ahn JK, Kim JJ, Chun SC, Kim EH, Seo SH, Kang EY, Kim SL, Park YJ, Ro HM, Chung IM. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J Agric Food Chem. 2008;56:7265-70. https://doi.org/10.1021/jf8008553
  10. Mizuno T. Bioactive substances in Hericium erinaceus and its medicinal utilization. Int J Med Mushrooms. 1999;1:105-19. https://doi.org/10.1615/IntJMedMushrooms.v1.i2.10
  11. Ma BJ, Shen JW, Yu HY, Ruan Y, Wu TT, Zhao X. Hericenones and erinacines: stimulators of nerve growth factor (NGF) biosynthesis in Hericium erinaceus. Mycology. 2010;1:92-8. https://doi.org/10.1080/21501201003735556
  12. Sokol S, Golak-Siwulska I, Sobieralski K., Siwulski M, Gorka K. Biology, cultivation, and medicinal functions of the mushroom Hericium erinaceum. Acta Mycol. 2015;50:1069-87.
  13. Choi JH, Kim HM, Song YS, Park SG, Kim JJ, Lee CK. Anti-aging effects Saccharomyces fermented modified Kyungohkgo extract on skin. The Korea Journal of Herbology. 2007;22(4):219-25.
  14. Park HB, Kim SY, Park SJ. Effects of Ginseng Sprout extract and modified Kyung-Ok-Ko on scopolamine-induced cognitive impairment in mice. Journal of Agricultural, Life and Environmental Sciences. 2019;31(3):151-9.
  15. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181:1199-200. https://doi.org/10.1038/1811199a0
  16. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant asay. Journal of Food Science and Technology. 2011;48(4):412-42. https://doi.org/10.1007/s13197-011-0251-1
  17. Shimamura T, Sumikura Y, Yamazaki T, Tada A, Kashiwagi T, Ishikawa H, Matsui T, Sugimoto N, Akiyama H, Ukeda H. Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives - inter-laboratory evaluation study. Analytical Sciences. 2014;30(7):717-21. https://doi.org/10.2116/analsci.30.717
  18. The Society of Korean Rehabilitation Medicine. Korean Rehabilitation Medicine. 4th ed. Paju:Koonja Publishing. 2015:3-9.
  19. Kim JB, Song HN. Effects of Kyeongok-go and its two added precriptions on hyperlipidemic rats induced by high-fat diet. Korean J Oriental Physiology & Pathology. 2014;28(4):371-8.
  20. Jung BK, Lee YJ, Yun HJ, Kang MS, Baek JH. The effect of KyungOcGogamibang on the growth of the rats. The Journal of Pediatrics of Korean Medicine. 2009;23(1):141-58.
  21. Do EJ, Hwang MY, Kim SY, Lee JS, Yang DS, Yang CH, Kim MR. The effect of Gyungokgo-gamibang extract on hair growth and protein expression in mice. Kor J Herbol. 2011;26(4):9-14. https://doi.org/10.6116/KJH.2011.26.4.009
  22. Lee ES, Seo BI, Lee JW, Bae JS. The immunological activities of Kyungohkgo and prescription of modified Kyungohkgo. Kor J Herbol. 2002;17(2):95-100.
  23. Na HJ, Jung YS. Immune homeostasis by diversity of regulatory T cell. Molecular and Cellular Biology Newsletter. [serial online] 2015;5(2):[7 screens]. Available from: http:www.ksmcb.or.kr/file/webzine/2015_05_02.pdf.
  24. Um SY. Chemical constituents of Hericium erinaceum for anticancer [dissertation]. Seoul:Sungkyunkwan University; 2012.
  25. Mizuno T, Wasa T, Ito H, Suzuki C, Ukai N. Antitumor active polysaccharides isolated from the fruiting body of Hericum erinaceus: an edible and medicinal mushroom called Yamabushitake or Houtou. Biosci Biotech Biochem. 1992;56:347-8. https://doi.org/10.1271/bbb.56.347
  26. Ryu SR, Lee WY, Ka KH. Comparative study on the sawdust cultivation and the antioxidant of Hericum spp. Kor J Mycol. 2009;37:80-5. https://doi.org/10.4489/KJM.2009.37.1.080
  27. Kawagishi H, Shimada A, Hosokawa S, Mori H, Sakamoto H, Ishiguro Y, Sakemi S, Bordner J, Kojima N, Furukawa S. Erinacines E, F and G stimulator of nerve growth factor (NGF)-synthesis from the mycelia of Hericum erinaceus. Tetrahedon Letters. 1996;37:7399-402. https://doi.org/10.1016/0040-4039(96)01687-5
  28. Lim CW, Kang KK, Yoo YB, Kim BH, Bae SH. Dietary fiber and β-glucan contents of Sparassis crispa fruit fermented with Lactobacillus brevis and Monascus pilosus. J Korean Soc Food Sci Nutr. 2012;41(12): 1740-6. https://doi.org/10.3746/jkfn.2012.41.12.1740
  29. Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka G, Nishooka I. Study on the inhibitory effects of tannins and flavonoids against the 1,1-diphenyl-2picrylhydrazyl radical. Biochemical Pharmacology. 1998;56:213-22. https://doi.org/10.1016/S0006-2952(98)00128-2
  30. Korea Food and Drug Administration Notice No. 2017-71 (2017-08-30) "Toxicity Test Standards, etc.".