DOI QR코드

DOI QR Code

Quality characteristics and bioactivity analysis of habanero red pepper (Capsicum chinense Jacq.) through acetic acid bacteria fermentation

하바네로 고추 초산발효물의 품질 특성 및 생리활성 분석

  • 박슬기 ((재)발효미생물산업진흥원) ;
  • 조승화 ((재)발효미생물산업진흥원) ;
  • 임은정 ((재)발효미생물산업진흥원) ;
  • 강현진 ((재)발효미생물산업진흥원) ;
  • 최동성 (우석대학교 식품생명영양학) ;
  • 정도연 ((재)발효미생물산업진흥원)
  • Received : 2021.09.17
  • Accepted : 2021.11.25
  • Published : 2021.12.31

Abstract

Habanero red pepper (HRP)-fermented liquid with Acetobacter pasteurianus SRCM101474 was prepared, the quality characteristics measured and bioactivity analysis were measured. As the fermentation period increased, the number of viable cells increased, pH decreased, and acidity increased. After fermentation, the organic acid content of HRP was confirmed to be acetic acid with the highest content of 33413.54 ppm. Analysis of capsaicin and dihydrocapsaicin showed that the increase was significantly raised as acetic acid fermentation progressed. The total phenol and flavonoid contents as well as the antioxidant activity increased as the fermentation period passed. In addition, α-glucosidase inhibitory and pancreatic lipase inhibitory activities were significantly increased after fermentation. It was confirmed that it can be used as a functional material by measuring the physiological activities of antioxidant, anti-obesity, and anti-diabetes through fermentation, and the possibility of development as a spicy material and application product using it as capsaicin increases after fermentation.

Acetobacter pasteurianus SRCM101474로 발효한 하바네로 고추 발효액을 제조하고, 품질분석 및 생리활성 분석을 측정하였다. 발효 기간이 증가함에 따라 생균수가 증가하였고, pH는 감소, 산도는 0.34%에서 3.18%로 증가하였다. 하바네로 고추 초산 발효물의 유기산 함량은 acetic acid, citric acid, succinic acid, oxalic acid 순으로 확인되었고, 발효 종료 시 acetic acid 함량이 33413.54 ppm으로 가장 높은 함량을 나타내었다. 매운맛 성분인 capsaicin과 dihydrocapsaicin 분석 결과 초산발효가 진행될수록 유의적으로 증가함을 보였다. 항산화 평가를 위해 총 페놀 및 플라보노이드 함량 및 DPPH, ABTS 라디칼 소거능을 측정한 결과 발효 기간이 지날수록 총 페놀함량 및 플라보노이드 함량이 증가하였고 항산화 활성도 유의적으로 증가하였다. 또한, α-glucosidase 억제 및 pancreatic lipase 억제 활성도 발효 후에 유의적으로 증가함을 보였다. 하바네로 고추의 초산 발효물의 항산화 활성 및 α-glucosidase, pancreatic lipase 억제 활성을 측정함으로써 기능성 소재로의 가능성을 확인하였고, 발효 후 capsaicin이 증가함에 따라 매운맛 소재 및 이를 활용한 응용제품으로서의 개발 가능성을 확인하였다.

Keywords

Acknowledgement

본 연구는 농림축산식품부 지역전략식품산업육성사업(2020-01) 지원에 의해 수행되었습니다.

References

  1. Ann YG, Kim SK, Shin CS. Studies on ginseng vinegar. Korean J. Food Nutr. 12: 447-454 (1999)
  2. Antonious GF, Lobel T, Kochhar T, Berke T, Jarret R. Antioxidants in Capsicum chinense: variation among countries of origin. J. Environ Sci Health 44: 621-626 (2009) https://doi.org/10.1080/03601230903000727
  3. Canadanovic-Brunet JM, Djilas SM, Cetkovic GS, Tumbas VT. Free-radical scavenging activity of wormwood (Artemisia absinthium L.) extracts. J. Sci. Food Agr. 85: 265-272 (2005) https://doi.org/10.1002/jsfa.1950
  4. Castro-Concha LA, Canche-Chuc I, MirandaHam MDL, Determination of antioxidants in fruit tissues from three accessions of habanero pepper (Capsicum chinense Jacq.), J. Mex. Chem. Soc. 56: 15-18 (2012)
  5. Castro-Concha LA, Tuyub-Che J, Moo-Mukul A, Vazquez-Flota FA, Miranda-Ham ML. Antioxidant capacity and total phenolic content in fruit tissues from accessions of Capsicum chinense Jacq.(Habanero pepper) at different stages of ripening. Sci. World J. 5: 140-145 (2014)
  6. Chung NH, Jo YH, Gao A, Gu SY, Jeong YJ, Kwon JH. Comparison of physicochemical properties and antioxidant activities of naturally-fermented commercial rice vinegars produced in Korea, China, and Japan. J. Korean Soc. Food Sci. Nutr. 44: 1799-1805 (2015) https://doi.org/10.3746/JKFN.2015.44.12.1799
  7. Furukawa S, Udea R. Studies on non-volatile organic acid in vinegar. contents of non-volatile organic acid in commercial vinegars. J. Ferment. Technol. 41: 14-19 (1963)
  8. Gang HM, Park HS, Rhim TJ, Kwon KR. A study on the comparison of antioxidant effects between hot pepper extract and capsaicin. J. Pharmacopuncture 11: 109-118 (2008) https://doi.org/10.3831/KPI.2008.11.1.109
  9. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: Chemistry, metabolism, and structure-activity relationships. J. Nutr. Biochem. 13: 572-584 (2002) https://doi.org/10.1016/S0955-2863(02)00208-5
  10. Hillebrand I, Boehme K, Frank G, Fink H, Berchtold P. The effects of the alpha-glucosidase inhibitor BAY g5421 (Acarbose) on meal-stimulated elevations of circurlating glucose, insulin, and triglyceride levels in man. Res. Exp. Med. (Berl.) 175: 81-86 (1979) https://doi.org/10.1007/BF01851236
  11. Hong SM, Moon HS, Lee JH, Lee HI, Jeong JH, Lee MK, Seo KI. Development of functional vinegar by using cucumbers. J. Korean Soc. Food Sci. Nutr. 41: 927-935 (2012) https://doi.org/10.3746/JKFN.2012.41.7.927
  12. Jarret RL, Berke T, Baldwin EA, Antonious GF. Variability for free sugars and organic acids in Capsicum chinense. Chem. Biodivers. 6: 138-145 (2009) https://doi.org/10.1002/cbdv.200800046
  13. Jeong YJ. Current trends and future prospects in the Korean vinegar industry. Food Sci. Ind. 42: 52-59 (2009) https://doi.org/10.23093/FSI.2009.42.2.52
  14. Keum JH. Studies on garlic and pumpkin vinegar. Korean J. Food Nutr. 12: 518-522 (1999)
  15. Kim HK, Kwon YJ, Kim KH, Jeong YH. Changes of total polyphenol content and electron donating ability of Aster glehni extracts with different microwave-assisted extraction conditions. Korean J. Food Sci. Technol. 32: 1022-1028 (2000)
  16. Labuza TP. Kinetics of lipid oxidation in foods. Crit. Rev. Food Technol. 2: 335-405 (1971)
  17. Lee CH, Chung KY, Lim SC, Choi DY, Kim CJ, Choi BK. Studies on the antioxidant activity of capsaicin and oleoresin from red pepper in grounded bacon belly meat. Korean J. Food Sci. Technol. 26: 496-499 (1994)
  18. Lee JH, Kim BH, Yoon YC, Kim JG, Park YE, Park HS, Lee JB. Effects against obesity and diabetes of red pepper (Capsicum annuum L.) fermented with lactic acid bacteria. J. Life Sci. 29: 354-361 (2019) https://doi.org/10.5352/JLS.2019.29.3.354
  19. Lee JS, Lee MG, Lee SW. A study on the general components and minerals in parts of omija (Schizandra chinensis Ballon). J. Korean Soc. Food Cult. 4: 173-176 (1989)
  20. Lee SH, Ro JS, Lee KS, Ahn YJ, Kang SJ, Hwang BY, Park WY, Ahn BT. The phenolic components of Sapium japonicum. Yakhak Hoeji 40: 183-192 (1996)
  21. Moon SY, Chung HC, Yoon HN. Comparative analysis of commercial vinegars in physicochemical properties, minor components and organoleptic tastes. Korean J. Food Sci. Technol. 29: 663-670 (1997)
  22. Na HS, Kim KS, Lee MY. Effect of jujube methanol extract on the hepeatoxicity in CCl4-treated tats. J. Korean Soc. Food Sci. Nutr. 25: 893-855 (1996)
  23. Oboh G, Puntel R.L, Rocha JBT. Hot pepper (Capsicum annuum, Tepin and Capsicum chinense, Habanero) prevents Fe2+-induced lipid peroxidation in brain in vitro. Food Chem. 102: 178-185 (2007) https://doi.org/10.1016/j.foodchem.2006.05.048
  24. Park TS, Kim DH, Kwon OJ, Son JH. A study on biological activities of fermented jujube and grape. Microbiol. Biotechnol. Lett. 42: 106-113 (2014) https://doi.org/10.4014/kjmb.1312.12003
  25. Pino J, Sauri-Duch E, Marbot R. Changes in volatile compounds of Habanero chili pepper (Capsicum chinense Jack. cv. Habanero) at two ripening stages. Food Chem. 94: 394-398 (2006) https://doi.org/10.1016/j.foodchem.2004.11.040
  26. Seo YJ, Kim JY, Noh SK. Effect of enteral capsaicin on the lymphatic absorption of cholesterol and fats in rats. J. Korean Soc. Food Sci. Nutr. 38: 1712-1717 (2009) https://doi.org/10.3746/JKFN.2009.38.12.1712
  27. Shin JY, Kang JR, Shin JH, Seo WT, Byun HU, Choi JS, Kang MJ. Effects of Seomaeyakssuk (Artemisia argyi H.) vinegar on lipid metabolism in rats fed a high-fat and high-cholesterol diet. J. Korean Soc. Food Sci. Nutr. 46(7): 779-789 (2017) https://doi.org/10.3746/JKFN.2017.46.7.779
  28. Shirataki Y, Kawase M, Saito S, Kurihara T, Tanaka W, Satoh K. Selective cytotoxic activity of grape peel and seed extracts against oral tumor cell lines. Anticancer Res. 20: 423-426 (2000)
  29. Shon MY. Antioxidant and anticancer activities of Poria cocos and Machilus thunbergii fermented with mycelial mushrooms. Food Ind. Nutr. 12: 51-57 (2007)
  30. Sosa-Moguel O, Pino J.A, Ayora-Talavera G, Sauri-Duch E, CuevasGlory L. Biological activities of volatile extracts from two varieties of Habanero pepper (Capsicum chinense Jacq.). Int. J. Food Prop. 20: S3042-S3051 (2017) https://doi.org/10.1080/10942912.2017.1397694
  31. Suzuki T, Iwai K. Constituents of red pepper spices: chemistry, biochemistry, pharmacology, and food science of the pungent principle of Capsicum species. The Alkaloids: Chem. Pharmacol. 263: 227-229 (1984) https://doi.org/10.1016/S0099-9598(08)60072-3
  32. Tood PH, Beninger MG, Biftu T. Determination of pungency due to capsicum by gas liquid chromatography. J. Food Sci. 42: 660-668 (1977) https://doi.org/10.1111/j.1365-2621.1977.tb12573.x
  33. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients 2: 1231-1246 (2010) https://doi.org/10.3390/nu2121231
  34. Van de Laar FA. Alpha-glucosidase inhibitors in the early treatment of type 2 diabetes. Vasc. Health Risk Manag. 4: 1189-1195 (2008) https://doi.org/10.2147/VHRM.S3119
  35. Watanabe J, Kawabata J, Kurihara H, Niki R. Isolation and identification of α-glucosidase inhibitors from tochucha (Eucommia ulmoides). Biosci. Biotech. Biochem. 61: 177-178 (1997) https://doi.org/10.1271/bbb.61.177
  36. Xu Q, Tao W, Ao Z. Antioxidant activity of vinegar melanoidins. Food Chem. 102: 841-849 (2007) https://doi.org/10.1016/j.foodchem.2006.06.013
  37. Yu JO, Choi WS, Lee US. Determination of capsaicin and dihydrocapsaicin in various species of red peppers and their powdered products in market by GC-MS analysis. Food Eng. Prog. 44: 1799-1805 (2009)