The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Study on Intestinal Flora and IgA Concentration Analysis in Newborn Mice by Stress and Diet in Pregnant Mice to Investigate the Effect of Taedok on the Intestinal Flora

태독이 장내 세균총에 미치는 영향을 규명하기 위한 임신쥐의 스트레스 및 식이에 따른 신생쥐의 장내 세균총 및 IgA 농도 분석 시험

  • Jeong, Jieun (Department of Pediatrics, College of Korean Medicine, Woosuk University) ;
  • Choi, Yoomin (Department of Acupuncture & Moxibustion, College of Korean Medicine, Woosuk University) ;
  • Jeong, Minjeong (Department of Pediatrics, College of Korean Medicine, Woosuk University)
  • 정지은 (우석대학교 한의과대학 소아과학교실) ;
  • 최유민 (우석대학교 한의과대학 침구의학교실) ;
  • 정민정 (우석대학교 한의과대학 소아과학교실)
  • Received : 2021.10.22
  • Accepted : 2021.11.19
  • Published : 2021.11.30
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Abstract

Objectives The purpose of this study is to look for pathological mechanism of disease development caused by Taedok, by studying whether stress and diet in pregnant ICR mice affect the intestinal flora and IgA (Immunoglobulin A) concentration. Methods The mice were divided into 4 groups (n=5 per group) based on the concept of Taedok: the control group (G1), stress group (G2), capsaicin diet group (G3), high fat diet group (G4). We collected and analyzed intestinal flora from maternal feces and cecal flora from neonatal mice by group. Then, IgA concentration in the maternal feces and sIgA (secretory Immunoglobulin A) concentration in the cecal contents of newborn mice were analyzed. In addition, serum corticosterone was analyzed before and after stress application. Results Changes in maternal intestinal flora and neonatal mice cecal flora by stress and diet were observed. There were no significant changes in the IgA concentration in maternal feces and the sIgA concentration in the cecal contents of neonatal mice. No significant changes compared to the control group were observed between groups before and after applying stress. However, when comparing within one subject, a significant increase was confirmed after stress application in the stress group (G2). Conclusions Based on the results, we observed stress and diet in pregnant mice affect the intestinal flora of maternal and neonatal. We were able to interpret the pathological mechanism of Taedok based on the principle of interaction between mother and newborn intestinal flora.

Keywords

Acknowledgement

이 논문은 2017년도 정부 (미래창조과학부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임 (NRF-2017R1C1B2011669).

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