Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Cultural characteristics of Bacillus velezensis HKB-1 in the water extract of the composted spent mushroom substrate of Lentinula edodes and biological control of Phytophthora blight disease of pepper

표고버섯 수확후배지 퇴비 추출물에서 Bacillus velezensis HKB-1의 배양적 특징 및 고추역병의 생물학적 방제

  • Kim, Ja-Yoon (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University) ;
  • Seo, Hyun-Ji (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University) ;
  • Kang, Dae-Sun (K-Global Ltd.) ;
  • Kang, Hee-Wan (School of Biotechnology, Division of Horticultural Biotechnology, Hankyong National University)
  • 김자윤 (한경대학교 생명공학부 원예생명공학전공) ;
  • 서현지 (한경대학교 생명공학부 원예생명공학전공) ;
  • 강대선 ((주)케이글로벌) ;
  • 강희완 (한경대학교 생명공학부 원예생명공학전공)
  • Received : 2021.11.30
  • Accepted : 2021.12.17
  • Published : 2021.12.31
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Abstract

Bacillus velezensis HKB-1 was isolated from the composted spent mushroom substrate of Lentinula edodes (LeCSMS) and inhibited mycelial growth of phytopathogenic fungal species, Phythhopthora capsici, Collectotrichum coccodes and Fusarium oxysporium by more than 70%. B. velezensis HKB-1 showed bacterial growth rate 10 to 100 times higher than that of other commercial bacterial media in water extract of LeCSMS supplemented with 1% molasses. The LeCSMS medium was effective in promoting the growth of pepper and controlling Phytophthora blight disease of pepper.

Bacillus velezensis HKB-1가 표고버섯 수확후배지 퇴비로부터 분리되었으며 고추역병균(Phythopthora capsici), 인삼모잘록병균(Rhizoctonia solani), 고추탄저병균(Collectotrichum coccodes) 및 시들음병(Fusarium oxysporium)의 균사체 성장을 70% 이상 억제하는 항 진균 활성을 보였다. B. velezensis HKB-1은 표고버섯 수확후배지 퇴비 물 추출물과 당밀 1% 첨가배지에서 다른 상업용 세균배지보다 10~100배 더 높은 세균증식률을 보였으며 고추 역병균의 균사체 생장을 90% 억제하였으며 고추생육 촉진효과 및 고추역병에 대하여 70% 이상의 방제효과가 있었다.

Keywords

Acknowledgement

본 연구는 농림수산식품기술기획평가원(IPET) 유용농생명자원산업화 기술개발사업(과제번호: 120043-2) 연구지원에 의해 수행된 결과입니다.

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