Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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A report of 18 unrecorded prokaryotic species isolated from the feces of an Oriental stork (Ciconia boyciana), and from the intestinal tracts of a cobitid fish (Kichulchoia multifasciata) and a Korean splendid dace (Coreoleuciscus splendidus)

  • Lee, So-Yeon (Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University) ;
  • Han, Jeong Eun (Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University) ;
  • Kim, Pil Soo (Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University) ;
  • Bae, Jin-Woo (Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University)
  • Received : 2020.03.26
  • Accepted : 2020.05.11
  • Published : 2020.11.30
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Abstract

The animal gut is filled with highly diverse microbes associated with host metabolism, physiology, and pathology. However, numerous animal gut microbes have not been cultured or reported. We isolated various bacterial species using culture-dependent approaches during a comprehensive investigation of endangered endemic vertebrate species in the Republic of Korea. A total of 18 unrecorded bacterial species were isolated from the feces of an Oriental stork (Ciconia boyciana), and from the intestinal tracts of a cobitid fish (Kichulchoia multifasciata) and a Korean splendid dace (Coreoleuciscus splendidus). Based on a phylogenetic analysis of 16S rRNA gene sequences, we discovered species belonging to the phyla Actinobacteria (eight species), Firmicutes (seven species), Proteobacteria (two species), and Bacteroidetes (one species). Based on their high 16S rRNA gene sequence similarities (>98.7%) and formation of monophyletic clades with type species, each species was classified into an independent and predefined bacterial species. Gram-stain reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and NIBR IDs for each species are described in the species description section.

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