대한본초학회지 (The Korea Journal of Herbology)

  • 제35권6호
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  • Pages.11-19
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  • 2020
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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감초 신품종 및 약전 수재감초의 면역조절 효과 비교 연구

The Comparative Study of Immunomodulatory Effect by Glycyrrhiza New Varieties and Official Compendia

  • 강윤미 (상지대학교 한의과대학 약리학교실) ;
  • 김원남 (세명대학교 한의과대학 본초방제학교실) ;
  • 진종식 (전북대학교 환경생명자원대학 한약자원학과) ;
  • 이종현 (동덕여자대학교 약학대학 약학과) ;
  • 장재기 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 안효진 (상지대학교 한의과대학 약리학교실)
  • Kang, Yun-Mi (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Kim, Wonnam (Division of Pharmacology, College of Korean Medicine, Semyung University) ;
  • Jin, Jong-Sik (Department of Oriental Medicine Resources, Jeonbuk National University) ;
  • Lee, Jong-Hyun (Department of Pharmacy, College of Pharmacy, Dongduk Women's University) ;
  • Chang, Jae Ki (Herbal Crop Research Division, NIHHS, RDA) ;
  • Lee, Jeonghoon (Herbal Crop Research Division, NIHHS, RDA) ;
  • An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University)
  • 투고 : 2020.10.08
  • 심사 : 2020.11.25
  • 발행 : 2020.11.30
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초록

Objective : The genus Glycyrrhiza has been used in food and traditional herbal medicine. Glycyrrhiza new varieties Wongam and Sinwongam have been developed by Korea Rural Development Administration and investigated to register on Korean Pharmacopoeia of the Ministry of Food and Drug Safety. The aim of this study is to investigate the immunomodulatory effect of Wongam and Sinwongam comparing with listed Glycyrrhiza species (Glycyrrhiza uralensis Fischer and G. glabra Linne) for evaluations about pharmacological effect of Glycyrrhiza new varieties. Methods : We studied the immunomodulatory effect of Wongam and Sinwongam compared with G. uralensis and G. glabra using THP-1 cell in vitro model. The cells were treated with phorbol 12-myristate 13-acetate (PMA) for differentiation and stimulated with lipopolysaccharides (LPS) to induce immune activation. We analyzed and compared the effects Glycyrrhiza new varieties and listed Glycyrrhiza species using nitric oxide (NO) assay, western blot, and reverse transcription-quantitative polymerase chain reaction analysis. 1) Results : Wongam and Sinwongam showed no cytotoxicity in THP-1 cells. Wongam and Sinwongam, and listed Glycyrrhiza species increased NO production, and cyclooxygenase (COX)-2 expression with or without LPS in differentiated THP-1 macrophages. Furthermore, Wongam and Sinwongam and listed Glycyrrhiza species upregulated the mRNA expressions of T helper type 1 (Th 1)-associated cytokines in LPS-stimulated THP-1 macrophages. Conclusion : These results indicated that Wongam and Sinwongam would have effect of enhancing immune response through the increase of NO and COX-2 expression, and activate Th1-associated cytokines. The findings of this study suggest the wide applicability of Glycyrrhiza new varieties.

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