한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제37권5호
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  • Pages.1356-1365
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  • 2020
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  • 1225-9098(pISSN)
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  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
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수국 꽃 추출물 유래 항산화 및 항염 활성 성분

Anti-oxidative and Anti-inflammatory Constituents from the Extracts of Hydrangea macrophylla Flowers

  • 조연정 ((사)제주산학융합원 연구개발팀) ;
  • 이용범 (제주한의약연구원 연구개발팀) ;
  • 현지선 ((사)제주산학융합원 연구개발팀) ;
  • 김창윤 ((사)제주산학융합원 연구개발팀) ;
  • 이남호 ((사)제주산학융합원 연구개발팀)
  • Jo, Yeon Jeong (R&D Team, Jeju Industry-University Convergence Center) ;
  • Lee, Yong bum (R&D Tem, Jeju Institute of Korean Medicine) ;
  • Hyun, Ji Seon (R&D Team, Jeju Industry-University Convergence Center) ;
  • Kim, Chang Yun (R&D Team, Jeju Industry-University Convergence Center) ;
  • Lee, Nam Ho (R&D Team, Jeju Industry-University Convergence Center)
  • 투고 : 2020.09.25
  • 심사 : 2020.10.30
  • 발행 : 2020.10.31
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초록

본 연구에서는 수국 꽃 추출물 및 용매 분획물의 항산화, 항염 활성을 확인하고 유효성분을 분리하여 화학구조를 규명하였다. 항산화 활성을 확인하기 위하여 DPPH 및 ABTS+ 라디칼 소거 활성을 측정하였으며, 항염 활성은 LPS로 유도된 RAW264.7 세포에서 nitric oxide (NO) 생성 억제 효과로 측정하였다. 실험 결과, 에틸아세테이트 분획물에서 우수한 항산화 및 항염 활성을 확인하였으며 크로마토크래피를 실시하여 3개의 유효 성분을 분리하였고 NMR 데이터 분석을 통하여 화학구조를 동정하였다; Hydrangenol (1), prunin (2), astragalin (3). 분리된 화합물 1-3에 대하여 HPLC 분석을 수행한 결과 hydrangenol이 수국 꽃 추출물의 주요 성분으로 확인되었다. 화합물 1-3의 항염 활성을 확인한 결과 세포 독성 없이 NO의 생성을 감소시키는 것으로 나타났다. 이상의 연구 결과를 바탕으로 수국 꽃 추출물은 천연 항산화 및 항염 소재로써 활용이 가능할 것으로 사료된다.

In this study, the extracts of Hydrangea macrophylla (H. macrophylla) flowers were investigated for the anti-oxidative and anti-inflammatory activities, and their active constituents were identified. The anti-oxidative effects were tested by DPPH and ABTS+ assays. To evaluate anti-inflammatory activities, LPS-induced RAW264.7 cells were examined. Among the extracts, the ethyl acetate fraction showed potent radical scavenging activities and inhibition of nitric oxide (NO) production. Chromatographic purification of the extract led to isolation of the compounds; hydrangenol (1), prunin (2) and astragalin (3). The chemical structures of the constituents were elucidated based on spectroscopic data including NMR spectra, as well as comparison of the data in the literature values. Quantitative analysis by high pressure liquid chromatography (HPLC) determined hydrangenol (1) as the major constituent. Isolated compounds 1-3 decreased the NO level without causing cell toxicities. Based on these results, it was suggested that the extract from H. macrophylla flowers could be potentially applicable as an anti-oxidative and/or anti-inflammatory ingredients.

키워드

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