Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Anti-oxidative Effect of Sapindus mukorossi Fruits Extract in LPS-stimulated macrophages via Activation of Nrf2/HO-1 pathway

LPS가 처리된 대식세포에서 Nrf2/HO-1 경로 활성을 통한 무환자나무 열매 추출물의 항산화 효과

  • 김대용 (우석대학교 한의과대학)
  • Received : 2020.10.07
  • Accepted : 2020.10.29
  • Published : 2020.10.31
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Abstract

The aims of this study were to determine the effects of Sapindus mukorossi fruit extracts (SME) on the anti-oxidant activity in LPS-stimulated RAW264.7 macrophages. The results showed that SME significantly reduced the production of ROS in LPS-stimulated RAW264.7 cells. The expression of pro-inflammatory proteins including COX-2 and iNOS were also obviously inhibited by SME in LPS-stimulated RAW264.7 cells. Further studies revealed that SME up-regulated HO-1 and Nrf2 expression. Additionally, SME increased phosphorylation of Akt and GSK-3β. These results suggest that SME could attenuate oxidative stress by activating the Nrf2/HO-1 signaling pathway.

본 연구의 목적은 LPS로 자극된 RAW264.7 대식세포에서 Sapindus mukorossi 열매 추출물(SME)의 항산화 효능을 확인하는 것이다. 그 결과, SME가 LPS로 자극된 RAW264.7 세포에서 ROS 생성을 현저하게 감소시켰고 전염증성 단백질인 COX-2 및 iNOS의 발현을 억제하였다. 또한 SME는 HO-1 및 Nrf2 발현을 상향 조절하였고 Akt 및 GSK-3β의 인산화를 증가시켰다. 이러한 결과는 SME가 Nrf2/HO-1 신호전달 경로의 활성화를 통하여 산화적 스트레스를 완화시킬 수 있음을 시사한다.

Keywords

References

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