Toxicological Research

  • 제36권4호
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  • Pages.337-342
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  • 2020
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
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DOI QR코드

DOI QR Code

Investigation of the genotoxic effects of fluoride on a bone tissue model

  • Volobaev, V.P. (Department of Genetics, Kemerovo State University) ;
  • Serdyukova, E.S. (Tomsk State University) ;
  • Kalyuzhnaya, E.E. (Department of Genetics, Kemerovo State University) ;
  • Schetnikova, E.A. (Department of Genetics, Kemerovo State University) ;
  • Korotkova, A.D. (Department of Genetics, Kemerovo State University) ;
  • Naik, A.A. (Department of Genetics, Kemerovo State University) ;
  • Bach, S.N. (Department of Genetics, Kemerovo State University) ;
  • Prosekov, A.Y. (Department of Genetics, Kemerovo State University) ;
  • Larionov, A.V. (Department of Genetics, Kemerovo State University)
  • 투고 : 2019.09.24
  • 심사 : 2020.01.23
  • 발행 : 2020.10.15
⟨ 이전 논문 다음 논문 ⟩

초록

Fluorides are thought to be a major cause of osteocarcinogenesis, due to their widespread industrial use, ability to accumulate in bone tissue, and genotoxic and probable carcinogenic properties. In vitro experiments investigating the genotoxic potential of fluorides in bone tissue models can provide valuable indirect information on their involvement in osteocarcinogenesis. Here, we investigated whether sodium fluoride (NaF) has the ability to induce DNA damage and chromosomal abnormalities in human osteosarcoma cells after 48 and 72 h of exposure. The cell cultures were treated with NaF in concentrations of 0, 20, 100 and 200 ㎍/ml. The level of DNA damage was assessed by the comet assay, and the frequency of chromosomal abnormalities by a micronucleus test. A significant increase in DNA damage indicators was noted in the samples treated with fluoride concentrations of 100 and 200 ㎍/ml, after 48 and 72 h of exposure. The micronucleus test revealed a dose-dependent increase in cells with micronuclei, nucleoplasmic bridges and nuclear protrusions. Increasing the concentration of NaF led to an increase in the prevalence of cytogenetic indicators after both treatment durations. This demonstrated ability of fluorine to exert genotoxic effects on bone cells indirectly indicates the possible importance of fluoride in the aetiology of osteosarcoma.

키워드

과제정보

The study was conducted with financial support from the Russian Foundation for Basic Research and the administration of the region of Kemerovo (No 18-44-420012 p_a, agreement with AKO No 8, 28 June 2018), and from a stipend for young scientists from the President of the Russian Federation.

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