Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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The Isoflavonoid Constituents and Biological Active of Astragalus Radix by Fermentation of β-glucosidase Strains

β-glucosidase 활성 균주 발효에 의한 황기 Isoflavonoid 성분변화 및 생리활성

  • Kim, Chul Joong (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Choi, Jae Hoo (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Seong, Eun Soo (Department of Medicinal Plant, Suwon Women's University) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Choi, Seon Kang (Department of Agricultural Life Sciences, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Lee, Jae Geun (Research Institute of Biotechnology, HwajinBioCosmetic)
  • 김철중 ((주)화진바이오코스메틱) ;
  • 최재후 ((주)화진바이오코스메틱) ;
  • 성은수 (수원여자대학교 약용식물과) ;
  • 임정대 (강원대학교 생약자원개발학과) ;
  • 최선강 (강원대학교 농생명산업학과) ;
  • 유창연 (강원대학교 생물자원과학과) ;
  • 이재근 ((주)화진바이오코스메틱)
  • Received : 2019.08.01
  • Accepted : 2020.09.09
  • Published : 2020.10.30
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Abstract

Background: In this study, the radix of Astragalus membranaceus Bunge extract fermented by Saccharomyces cerevisiae, Weissella cibaria, and Pediococcus pentosaceus to increase the levels of isoflavonoid aglycone contents. Methods and Results: In order to change the in isoflavonoids, we fermented the radix of A. membranaceus extracts with microorganisms that have β-glucosidase activity. Besed on the β-glucosidase activity, we selected three strains, Weissella cibaria, Pediococcus pentosaceus, and Saccharomyces cerevisiae. HPLC analysis revealed that the levels of isoflavonoid aglycones were increased in all fermentation cases, and the extracts fermented by S. cerevisiae showed the highest levels of isoflavonoid aglycones. We evaluated the antioxidant activity, anti-wrinkle effects and whitening effects of the S. cerevisiae-fermented extracts using the DPPH assay, tyrosinase inhibition activity assay, and collagenase inhibition activity assay. We confirmed higher activity in S. cerevisiae-fermented extracts than in control, with the half maximal inhibitory concentration (IC50) value of 565.1 ± 59.1 ㎍/㎖ in DPPH radical scavenging activity, tyrosinase inhibition rate of 78.4 ± 0.9%, and collagenase inhibition rate of 83.8 ± 1.1%. Conclusions: We selected three stains of microorganisms showing high β-glucosidase activity, W. cibaria, P. pentosaceus and S. cerevisiae. Isoflavonoid glycones in the radix of A. membranaceus were converted to isoflavonoid aglycones by fermentation. In addition, the fermented radix of A. membranaceus exhibited antioxidant activity, anti-wrinkle effect, whitening effect and radical scavenging activity.

Keywords

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