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배추 유전자원의 항산화 효과

Antioxidant Activity of Chinese Cabbage (Brassica rapa L. ssp. pekinensis) using Different Genotypes

  • 황병순 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김지영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 권수현 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김기창 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 강해주 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 이주희 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 황인국 (농촌진흥청 국립농업과학원 농식품자원부)
  • Hwang, Byung Soon (Dept. of Agrofood Resources, National Institute of Agricultural Science, RDA) ;
  • Kim, Ji Yeong (Dept. of Agrofood Resources, National Institute of Agricultural Science, RDA) ;
  • Kwon, Su Hyun (Dept. of Agrofood Resources, National Institute of Agricultural Science, RDA) ;
  • Kim, Gi-Chang (Dept. of Agrofood Resources, National Institute of Agricultural Science, RDA) ;
  • Kang, Hae Ju (Dept. of Agrofood Resources, National Institute of Agricultural Science, RDA) ;
  • Rhee, Ju Hee (National Agrobiodiversity Center, National Institute of Agricultural Science, RDA) ;
  • Hwang, In Guk (Dept. of Agrofood Resources, National Institute of Agricultural Science, RDA)
  • 투고 : 2020.09.11
  • 심사 : 2020.09.29
  • 발행 : 2020.10.31

초록

This experiment was conducted to assess the high antioxidant activity varieties of Chinese cabbage (Brassica rapa L. ssp. pekinensis) from the 55 accessions. The antioxidant activity of Chinese cabbage were determined by the TPC, TFC, DPPH, ABTS, and chlorophyll, carotenoid contents. The TPC and TFC showed a range of 1.21~4.61 mg GAE/g DW, 0.18~3.09 mg CE/g DW. The DPPH and ABTS assay were in the range of 0.65~4.36 and 1.42~6.91 mg ascorbic acid equivalent (ASCE)/g DW, respectively. The UPLC analysis was performed quantitatively to identify chlorophyll and carotenoid in the Chinese cabbage extract. The levels of the total chlorophyll and total carotenoid were 86.60~1,235.91, and 75.86~490.11 ㎍/g, respectively. The comprehensive differences in the total and individual chlorophyll contents have also been observed among different varieties. These results will be valuable as basic data for the standardization of Chinese cabbage.

키워드

참고문헌

  1. An TH, See SY, Cho JW. 2012. Comparison on antioxidant activity of ethanolic extracts of Chinese matrimony vine (Lycium chinensis M.). Korean J Crop Sci 57:22-28 https://doi.org/10.7740/kjcs.2012.57.1.022
  2. Ancerewicz J, Migliavacca E, Carrupt PA, Testa B, Bree F, Zini R, Tillement JP, Labidalle S, Guyot D, Chauvet-Monges AM, Crevat A, Ridant AL. 1998. Structure-property relationships of trimetazidine derivatives and model compounds as potential antioxidants. Free Radical Biol Med 25:113-120 https://doi.org/10.1016/S0891-5849(98)00072-0
  3. Baek SA, Jung YH, Lim SH, Park SU, Kim JK. 2016. Metabolic profiling in Chinese cabbage (Brassica rapa L. subsp. pekinensis) cultivars reveals that glucosinolate content is correlated with carotenoid content. J Agric Food Chem 64:4426-4434 https://doi.org/10.1021/acs.jafc.6b01323
  4. Cartea ME, Francisco M, Soengas P, Velasco P. 2011. Phenolic compounds in Brassica vegetables. Molecules 16:251-280 https://doi.org/10.3390/molecules16010251
  5. Cha WS, Shin HR, Park JH, Oh SL, Lee WY, Chun SS, Choo JW, Cho YJ. 2004. Antioxidant activity of phenol compounds from mulberry fruits. Korean J Food Preserv 11:383-387
  6. Choi YH, Kim JS, Seo JH, Lee JW, Kim YS, Ryu SY, Lee KR, Kim YK, Kim SH. 2004. Chemical constituents of Brassica campestris ssp. pekinensis. Korean J Pharmacogn 35:255-258
  7. Dewanto V, Wu X, Liu RH. 2002. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50:4959-4964 https://doi.org/10.1021/jf0255937
  8. Edelenbos M, Christensen LP, Grevsen K. 2001. HPLC determination of chlorophyll and carotenoid pigments in processed green pea cultivars (Pisum sativum L.). J Agric Food Chem 49:4768-4774 https://doi.org/10.1021/jf010569z
  9. Ge Y, Wang T, Wang N, Wang Z, Liang C, Ramchiary N, Choi SR, Lim YP, Piao ZY. 2012. Genetic mapping and localization of quantitative trait loci for chlorophyll content in Chinese cabbage (Brassica rapa ssp. pekinensis). Sci Hortic 147:42-48 https://doi.org/10.1016/j.scienta.2012.09.004
  10. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. 1993. Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen elderly study. Lancet 342:1007-1011 https://doi.org/10.1016/0140-6736(93)92876-U
  11. Hong EY, Kim SJ, Kim GH. 2011. Identification and quantitative determination of glucosinolates in seeds and edible parts of Korean Chinese cabbage. Food Chem 128:1115-1120 https://doi.org/10.1016/j.foodchem.2010.11.102
  12. Hwang ES, Hong E, Kim GH. 2012. Determination of bioactive compounds and anti-cancer effect from extracts of Korean cabbage and cabbage. Korean J Food Nutr 25:259-265 https://doi.org/10.9799/ksfan.2012.25.2.259
  13. Hwang IG, Kim HY, Park BR, Han HM, Yoo SM. 2013. Effect of heat treatment on the antioxidant properties of Yacon (Smallanthus sonchifolius). Korean J Food Nutr 26:857-864 https://doi.org/10.9799/ksfan.2013.26.4.857
  14. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS. 2006. Change of physicochemical characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J Food Sci Technol 38:342-347
  15. Jeong YS, Lee SH, Song J, Hwang KA, Noh GM, Jang DE, Hwang IG. 2016. Phytochemical contents and antioxidant activities of Opuntia ficus-indica var. saboten. Korean J Food Nutr 29:767-776 https://doi.org/10.9799/ksfan.2016.29.5.767
  16. Kim HW, Kim JB, Poovan S, Chung MN, Cho SM, Lee YM, Cho YS, Kim JH, Kim HR. 2014. Effect of processing conditions on the content of cis/trans carotene isomers as provitamin A carotenoids in Korean sweet potato varieties. Int J Food Sci Nutr 65:821-826 https://doi.org/10.3109/09637486.2013.854742
  17. Kim JY, Park YH, Park SE, Hwang BS, Hwang IG, Kim GC. 2020. Antioxidant activity and phenolic acid analysis of Rudbeckia lanciniata var. hortensis extract. J Korean Soc Food Sci Nutr 49:46-53 https://doi.org/10.3746/jkfn.2020.49.1.46
  18. Kim SH, Chung MJ, Jang HD, Ham SS. 2010. Antioxidative activities of the Codonopsis lanceolata extract in vitro and in vivo. J Korean Soc Food Sci Nutr 39:193-202 https://doi.org/10.3746/jkfn.2010.39.2.193
  19. Kopsell DA, Kopsell DE, Lefsrud MG, Curran-Celentano J, Dukach LE. 2004. Variation in lutein, B-carotene, and chlorophyll concentrations among Brassica oleracea cultigens and seasons. HortScience 39:361-364 https://doi.org/10.21273/HORTSCI.39.2.361
  20. Lee MK, Cho SY, Cho SJ, Shin JH, Kim HW, Kim SG, Ko HC, Ro NY, Kim JB, Baek HJ. 2015. Changes in carotenoid contents of colored pumpkin (Cucurbita spp.) germplasms. Korean J Environ Agric 34:186-191 https://doi.org/10.5338/KJEA.2015.34.3.22
  21. Lee MK, Chun JH, Byeon DH, Chung SO, Park SU, Park S, Arasu MV, Al-Dhabi NA, Lim YP, Kim SJ. 2014. Variation of glucosinolates in 62 varieties of Chinese cabbage (Brassica rapa L. ssp. pekinensis) and their antioxidant activity. LWT-Food Science Technol 58:93-101 https://doi.org/10.1016/j.lwt.2014.03.001
  22. Lee YS, Joo EY, Kim NW. 2006. Polyphenol contents and antioxidant activity of Lepista nuda. J Korean Soc Food Sci Nutr 35:1309-1314 https://doi.org/10.3746/jkfn.2006.35.10.1309
  23. Seo MS, Shon SH, Park BS, Ko HC, Jin M. 2014. Efficiency of microspore embryogenesis in Brassica rapa using different genotypes and culture conditions. J Plant Biotechnol 41:116-122 https://doi.org/10.5010/JPB.2014.41.3.116
  24. Seong GU, Hwang IW, Chung SK. 2016. Antioxidant capacities and polyphenolics of Chinese cabbage (Brassica rapa L. ssp. pekinensis) leaves. Food Chem 199:612-618 https://doi.org/10.1016/j.foodchem.2015.12.066
  25. Seong GU, Kim JB, Chung SK. 2018. Carotenoid contents of head-type Kimchi cabbage (Brassica rapa L. ssp. pekinensis) leaves. J Korean Soc Food Sci Nutr 47:912-916 https://doi.org/10.3746/jkfn.2018.47.9.912
  26. Seong JH, Park SG, Park EM, Kim HS, Kim DS, Chung HS. 2006. Contents of chemical contituents in organic Korean cabbages. Korean J Food Preserv 13:655-660
  27. Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  28. Zou Z, Ishida M, Li F, Kakizaki T, Suzuki S, Kitashiba H, Nishio T. 2013. QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L. PLOS ONE 8:e53541 https://doi.org/10.1371/journal.pone.0053541