Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

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Embryo Production from Elk using Ultrasound-Guided Ovum Pick-Up Technique

초음파 유래 Ovum Pick-Up 기술을 이용한 엘크 암사슴의 수정란 생산

  • Lee, Eun-Do (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Hoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Kyo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Jinwook (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sung-Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Kwan-Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 이은도 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이상훈 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김동교 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이진욱 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이성수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김관우 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2020.09.08
  • Accepted : 2020.10.05
  • Published : 2020.10.31
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Abstract

This study, which was designed to improve and increase the utilization of female elk, examined the possibility of collecting their embryos during the non-reproductive period using ultrasound-guided Ovum Pick-Up (OPU) technology. Once follicles in the ovaries of an elk were observed, the embryos were retrieved through the absorption of the follicles using an OPU ultrasound probe. Fifty-seven embryos were retrieved from 85 follicles, giving a retrieval rate of 67.1%. Morphological evaluation of the recovered embryos revealed 14.0% embryos to be in grade A, 19.2% in grade B, 15.7% in grade C, and 50.8% in grade D. The developmental efficiency of the retrieved embryos was also investigated using in vitro maturation, in vitro fertilization, and in vitro culture. After cultivating 28 embryos through in vitro fertilization, 19 embryos were found to be fertilized; the fertilization rate was 67.9%. Four embryos developed to the blastocyst stage, indicating a 14.3% development rate. This study confirmed that the production of fertilized embryos from a seasonal breeder, such as elk, is possible via the ultrasound-guided OPU method. If the efficiency of in vitro fertilization and in vitro culture can be improved through further research, it will help improve the efficiency of elk embryo production through the transplantation of their fertilized embryos.

본 연구는 초음파 유래 OPU (Ovum Pick-Up) 기술을 이용하여 엘크 암사슴의 개량 및 활용성 증진을 위해 엘크 암사슴 난자를 채취하였다. 비번식 계절시기에도 체외 수정란이 생산 가능한지 확인하기 위하여 OPU 초음파 진단기를 이용하여 사슴 난소에서 난포를 흡입한 후 난자를 회수 하였다. 총 85개의 난포를 흡입 한 결과 57개의 난자가 회수되어 67.1%의 회수율을 나타내었다. 난자의 등급을 평가한 결과 A 등급 14.0%, B 등급 19.2%, C 등급 15.7% 및 D 등급 50.8%로 조사되었다. 본 연구에서는 A~C 등급의 난자를 이용하여 체외성숙, 체외수정 및 체외배양을 실시하였다. 총 28개의 난자를 이용하여 67.9% (19/28)의 수정율을 보였으며 배반포 발달율은 14.3% (4/28)로 나타났다. 본 연구 결과를 토대로 하여 볼 때, 계절번식을 하는 것으로 알려져 있는 엘크에 초음파 유래 OPU 방법이 적용 가능한 것으로 확인하였다. 따라서, 향후 추가적인 연구를 통해 체외수정과 체외배양 효율을 보다 향상시킨다면 엘크 수정란 이식을 통해 사슴의 개량기반 구축과 암사슴의 활용성 향상에 기여 할 수 있을 것으로 사료된다.

Keywords

References

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