한국균학회지 (The Korean Journal of Mycology)

  • 제48권3호
  • /
  • Pages.329-335
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  • 2020
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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형태적 특징 및 다좌위 염기서열 분석에 의한 산철쭉 모무늬병균 Sphaerulina azaleae 동정

Identification of Sphaerulina azaleae on Korean Azalea in Korea Based on Morphological Characteristics and Multilocus Sequence Typing

  • 최인영 (전북대학교 농생물학과) ;
  • 최영준 (군산대학교 생물학과) ;
  • 이귀재 (전북대학교 환경생명자원대학) ;
  • 주호종 (전북대학교 농생물학과) ;
  • 조성완 (건양대학교 제약생명학과) ;
  • 신현동 (고려대학교 환경생태공학부)
  • Choi, In-Young (Department of Agricultural Biology, Jeonbuk National University) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University) ;
  • Lee, Kui-Jae (Division of Biotechnology, College of Environmental and Bioresource Sciences, Jeonbuk National University) ;
  • Ju, Ho-Jong (Department of Agricultural Biology, Jeonbuk National University) ;
  • Cho, Seong-Wan (Department of Pharmaceutics & Biotechnology, Konyang University) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
  • Received : 2020.08.25
  • Accepted : 2020.09.21
  • Published : 2020.09.30
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Abstract

2008년부터 2017년도에 제주, 홍천 등에서 산철쭉에서 모무늬 증상을 나타내는 잎을 채집하였다. 산철쭉모무늬 증상은 빈번하게 잎에만 발생하여 식물의 관상가치를 떨어트리고 조기낙엽을 유발하였다. 변색부는 잎의 윗면에 작고 담갈색 내지 흑자색 점무늬가 먼저 나타나며, 잎의 세맥으로 경계가 구분되어 모무늬 또는 부정형의 증상을 나타냈다. 산철쭉에서 분리한 균주를 동정하고자 형태적 특징과 actin (Act), translation elongation factor 1-alpha (EF), internal transcribed spacer (ITS), 28S nrDNA (LSU), RNA polymerase II second largest subunit (RPB2) 염기서열을 분석하였다. 형태적 특징을 재확인한 염기서열 분석결과 Sp. azaleae와 99~100%의 상동성을 나타냈으며, 계통수를 작성하였을 때도 Sp. azaleae 계통군에 속하였다. 따라서 산철쭉에 모무늬 증상에 관여하는 곰팡이는 Sp. azaleae로 동정되었다.

From 2008 to 2017, Korean azalea (Rhododendron yedoense f. poukhanense) showing angular, necrotic leaf spots were found in Jeju and Hongcheon, Korea. The lesions occurred frequently, detracting from the beauty of the glossy green leaves of the plant and causing premature defoliation. Therefore, to identify the fungus associated with the lesions, morphological characterization and molecular phylogenetic analysis of actin (Act), translation elongation factor 1-alpha (EF), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II encoding the second largest subunit (RPB2) of the two representative isolates were performed. The phylogenetic tree inferred from the neighbor-joining method showed the isolates clustering in the Sphaerulina azaleae group. Therefore, the fungus associated with the angular leaf spots on the Korean azalea was identified as Sphaerulina azaleae.

Keywords

References

  1. Farr DF, Rossman AY. Fungal databases. Systematic mycology & microbiology laboratory, ARS, USDA. Internet Resource; 2020 [cited 2020 Jul 24] Available from: http://ntars-gringov/fungaldatabases/.
  2. Quaedvlieg W, Verkley GJN, Shin HD, Barreto RW, Alfenas AC, Swart WJ, Groenewald JZ, Crous PW. Sizing up Septoria. Stud Mycol 2013;75:307-90. https://doi.org/10.3114/sim0017
  3. Verkley GJM, Quaedvlieg W, Shin HD, Crous PW. A new approach to species delimitation in Septoria. Stud Mycol 2013;75:213-305. https://doi.org/10.3114/sim0018
  4. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999;91:553-6. https://doi.org/10.1080/00275514.1999.12061051
  5. O'Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Nat Acad Sci 1998;95:2044-9. https://doi.org/10.1073/pnas.95.5.2044
  6. Liu YJ, Whelen S, Hall BD. Phylogenetic relationships among ascomycetes: Evidence from an RNA polymerse II subunit. Mol Biol Evol 1999;16:1799-808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  7. Quaedvlieg W, Kema GHJ, Groenewald JZ, Verkley GJM, Seifbarghi S, Razavi M, Gohari AM, Mehrabi R, Crous PW. Zymoseptoria gen. nov.: A new genus to accommodate Septorialike species occurring on graminicolous hosts. Persoonia: Mol Phylog Evol Fungi 2011;26:57-69. https://doi.org/10.3767/003158511X571841
  8. Crous PW, Schoch CL, Hyde KD, Wood AR, Gueidan C, De Hoog GS, Groenewald JZ. Phylogenetic lineages in the Capnodiales. Stud Mycol 2009;64:17-47. https://doi.org/10.3114/sim.2009.64.02
  9. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46. https://doi.org/10.1128/JB.172.8.4238-4246.1990
  10. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications 1990;18:315-22.
  11. Groenewald JZ, Nakashima C, Nishikawa J, Shin HD, Park JH, Jama AN, Groenewald M, Braun U, Crous PW. Species concepts in Cercospora: Spotting the weeds among the roses. Stud Mycol 2013;75:115-70. https://doi.org/10.3114/sim0012
  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  13. Saccardo PA, Sydow P. Septoria azaleae Vogl. Italy: In Sylloge Fungorum XIV Patavii; 1898; p. 976.
  14. White RP. The insects and diseases of rhododendron and azalea. J Econ Entomol 1933;26:631-40. https://doi.org/10.1093/jee/26.3.631
  15. Hemmi T, Kurata S, Nakamura H, Hirayama S, Satoh S. Studies on septorioses of plants. II. Septoria azaleae Voglino causing the brown-spot disease of the cultivated azaleas in Japan. Memoirs Coll Agr Kyoto Imp Univ 1931;13:1-22.
  16. Priest MJ. Fungi of Australia: Septoria. Canberra: ABRS CSIRO Publishing; 2006.
  17. Crous PW, Tanaka K, Summerell BA. Additions to the Mycosphaerella complex. IMA Fungus 2001;2:49-64. https://doi.org/10.5598/imafungus.2011.02.01.08
  18. The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.