The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Diversity and Pathogenic Characteristics of Fusarium Species isolated from Wilted Soybeans in Korea

콩 시들음병에 관여하는 Fusarium균의 다양성 및 병원학적 특성

  • Choi, Hyo-Won (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kim, Seungnoh (Taean Agricultural Technology Center) ;
  • Hong, Sung Kee (Crop Protection Division, National Institute of Agricultural Sciences)
  • 최효원 (국립농업과학원 작물보호과) ;
  • 김승노 (태안군 농업기술센터) ;
  • 홍성기 (국립농업과학원 작물보호과)
  • Received : 2020.09.01
  • Accepted : 2020.09.23
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Wilted soybean plants were collected from soybeans cultivation fields in Korea from 2014 to 2016. Fusarium spp., Colletotrichum spp., Rhizoctonia spp., Macrophomina sp., Phytophthora spp., and Calonectria ilicicola were obtained from the infected samples. Out of these, Fusarium spp. were the dominant species (79.1%). In total, 53 isolates were identified as F. solani species complex, F. oxysporum species complex, F. graminearum species complex, and F. fujikuroi species complex based on mycological characteristics. Sequence typing analysis was conducted using translation elongation factor 1 alpha (TEF) to confirm the identification of isolates. All isolates were identified as F. solani, F. oxysporum, F. commune, F. asiaticum, and F. fujikuroi based on phylogenetic analysis of TEF sequences. Pathogenicity of 44 isolates was tested on three cultivars of soybean using the root dip inoculation method. Out of 5 Fusarium species, only F. asiaticum could not cause the symptoms or be weak. Ten isolates were selected based on pathogenic characters and species identification to investigate the host range and screen soybean cultivars for resistance. Fusarium solani, F. oxysporum, and F. commune were aggressive only to soybean, and F. fujikuroi was aggressive to kidney bean, yellow cowpea, black cowpea, adzuki bean as well as soybean. All 13 Korean soybean cultivars were susceptible to F. commune and F. fujikuroi. Out of 13 cultivars, cv. Janggi, cv. Poongsannamul, and cv. Socheongja were resistant to Fusarium wilt, while cv. Hwanggeumol and Chamol were susceptible to Fusarium wilt.

2014년부터 2016년까지 국내 콩 재배 포장에서 시들음 증상을 보이는 시료에서 진균을 분리한 결과, Fusarium spp., Colletotrichum spp., Rhizoctonia spp., Macrophomina sp., Phytophthora spp., Calonectria ilicicola가 분리되었고, Fusarium균이 79.1%로 가장 많이 분리되었다. 53개의 Fusarium균을 균학적 특성에 의해 동정한 결과, F. solani, F. oxysporum, F. graminearum, F. fujikuroi 등 4개의 종 복합체(species complex)로 동정되었다. 염기서열분석을 통한 종 동정을 위해 translation elongation factor 1 alpha (TEF) 유전자 부위를 계통분석한 결과, F. solani, F. oxysporum, F. commune, F. asiaticum, F. fujikuroi 등 5종으로 확인되었다. 44개 Fusarium균주의 병원성을 확인하기 위하여 콩 3개 품종을 대상으로 유묘 침지 접종한 결과, F. asiaticum은 병원성이 없거나 미미한 것으로 나타났다. 병원성이 있는 10개 균주를 선발하여 기주범위를 조사한 결과, F. solani, F. oxysporum, F. commune은 콩에만 병원성이 있었고, F. fujikuroi는 콩, 강낭콩, 황동부콩, 흑동부콩 및 팥에 병원성을 보였다. 또한 13개 콩 품종을 대상으로 저항성 검정을 수행한 결과, F. commune과 F. fujikuroi는 모든 품종에 병을 일으켰다. 장기콩, 풍산나물콩, 소청자콩은 Fusarium에 의한 시들음병에 비교적 저항성이 것으로 나타났고, 황금올콩, 참올콩은 감수성 품종으로 확인되었다.

Keywords

References

  1. Bandara AY, Weerasooriya DK, Bradley CA, Allen TW, Esker PD. Dissecting the economic impact of soybean diseases in the United States over two decades. PLoS One 2020;15:e0231141. Doi:10.1371/journal.pone.0231141.
  2. Korean Statistical Information Service. Statistical Database [Internet]. Daejeon: Korean Statistical Information Service; 2020 [cited 2020 Jun. 26]. Available from http://kosis.kr/index/index.do.
  3. Moon YG, Lee JH, Choi JK, Kang AS, Han SS. Yield loss assessment and determination of economic thresholds limits against soybean anthracnose. Korean J Pestic Sci 2010;14:133-7.
  4. Shim H, Lee JH, Lee YH, Myung IS, Choi HW. Establishment of economic threshold by evaluation of yield component and yield damages caused by leaf spot disease of soybean. Res Plant Dis 2013;19:196-200. https://doi.org/10.5423/RPD.2013.19.3.196
  5. Hong SJ, Kim YK, Jee HJ, Shim CK, Kim MJ, Park JH, Han EJ, Lee BC. Influence of disease severity of bacterial pustule caused by Xanthomonas axonopodis pv. glycines on soybean yield. Res Plant Dis 2011;17:317-25. https://doi.org/10.5423/RPD.2011.17.3.317
  6. Allen TW, Bradley CA, Sisson AJ, Byamukama E, Chilvers MI, Coker CM, Collins AA, Damicone JP, Dorrance AE, Dufault NS, et al. Soybean yield loss estimates due to diseases in the United States and Ontario, Canada, from 2010 to 2014. Plant Health Prog 2017:18:19-27. https://doi.org/10.1094/PHP-RS-16-0066
  7. Ko YM, Choi J, Lee YH, Kim HT. First report of charcoal rot caused by Macorphomina phaseolina on Glycine max in Korea. Res Plant Dis 2020;26:29-37. https://doi.org/10.5423/RPD.2020.26.1.29
  8. Diaz Arias MM, Leandro LF, Munkvold GP. Aggressiveness of Fusarium species and impact of root infection on growth and yield of soybeans. Phytopathology 2013;103:822-32. https://doi.org/10.1094/PHYTO-08-12-0207-R
  9. Chang X, Dai H, Wang D, Zhou H, He W, Fu Y, Ibrahim F, Zhou Y, Gong G, Shang J, et al. Identification of Fusarium species associated with soybean root rot in Sichuan province, China. Eur J Plant Pathol 2018;151:563-77. https://doi.org/10.1007/s10658-017-1410-7
  10. Zhang JX, Xue AG, Cober ER, Morrison MJ, Zhang HJ, Zhang SZ, Gregorich E. Prevalence, pathogenicity and cultivar resistance of Fusarium and Rhizoctonia species causing soybean root rot. Can J Plant Sci 2013;93:221-36. https://doi.org/10.4141/cjps2012-223
  11. Choi HW, Hong KS, Kim JS, Lee YK. First report of Fusarium fujikuroi causing Fusarium wilt on Glycine max in Korea. Plant Dis 2019;103:2469.
  12. Kang IJ, Shim HK, Heu S, Kim KS. First report of soybean root and stem rot caused by Fusarium graminearum in South Korea. Plant Dis 2020;104:568. https://doi.org/10.1094/PDIS-07-19-1504-PDN
  13. Gerlach W, Nirenberg H. The genus Fusarium: A pictorial atlas. Berlin: Kommissionsverlag P. Parey; 1982.
  14. Leslie JF, Summerell BA. The Fusarium laboratory manual. New York: John Wiley & Sons; 2006.
  15. Choi HW, Kim JM, Hong SK, Kim WG, Chun SC, Yu SH. Mating types and optimum culture conditions for sexual state formation of Fusarium fujikuroi isolates. Mycobiology 2009;37:247-50. https://doi.org/10.4489/MYCO.2009.37.4.247
  16. O'Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 1998;95:2044-9. https://doi.org/10.1073/pnas.95.5.2044
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  18. Ye W, Zeng D, Xu M, Yang J, Ma J, Wang Y, Zheng X. A LAMP-assay-based specific microbiota analysis reveals community dynamics and potential interactions of 13 major soybean root pathogens. J Integr Agric 2020;19:2056-63. https://doi.org/10.1016/S2095-3119(19)62855-8
  19. Okello PN, Petrovic K, Singh AK, Kontz B, Mathew FM. Characterization of species of Fusarium causing root rot of soybean (Glycine max L.) in South Dakota, USA. Can J Plant Pathol 2020. https://doi.org/10.1080/07060661.2020.1746695.
  20. Farias GM, Griffin GJ. Roles of Fusarium oxysporum and Fusarium solani in Essex disease of soybean in Virginia. Plant Dis 1989;73:38-42. https://doi.org/10.1094/PD-73-0038
  21. Kolander TM, Bienapfl JC, Kurle JE, Malvick DK. Symptomatic and asymptomatic host range of Fusarium virguliforme, the causal agent of soybean sudden death syndrome. Plant Dis 2012;96:1148-53. https://doi.org/10.1094/PDIS-08-11-0685-RE