Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Probiotic Potential of Pediococcus acidilactici and Enterococcus faecium Isolated from Indigenous Yogurt and Raw Goat Milk

  • Sarkar, Shovon Lal (Department of Microbiology, Jashore University of Science and Technology) ;
  • Hossain, Md. Iqbal (Department of Microbiology, Jashore University of Science and Technology) ;
  • Monika, Sharmin Akter (Department of Microbiology, Jashore University of Science and Technology) ;
  • Sanyal, Santonu Kumar (Department of Microbiology, Jashore University of Science and Technology) ;
  • Roy, Pravas Chandra (Department of Microbiology, Jashore University of Science and Technology) ;
  • Hossain, Md. Anwar (Department of Microbiology, University of Dhaka) ;
  • Jahid, Iqbal Kabir (Department of Microbiology, Jashore University of Science and Technology)
  • Received : 2019.12.15
  • Accepted : 2020.03.09
  • Published : 2020.09.28
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Abstract

Probiotics are live microorganisms that, when administered in adequate amounts, confer health benefits to the host. This study was conducted for the isolation of potential lactic acid bacteria (LAB) with probiotic properties from goat milk and yogurt. Several tests were conducted in vitro using the standard procedures for evaluating the inhibitory spectra of LAB against pathogenic bacteria; tolerance to NaCl, bile salt, and phenol; hemolytic, milk coagulation, and bile salt hydrolase activities; gastrointestinal transit tolerance; adhesion properties; and antibiotic susceptibility. Among 40 LAB strains screened according to culture characteristics, five isolates exhibited antagonistic properties. Three were identified as Pediococcus acidilactici, and two were identified as Enterococcus faecium, exploiting 16S rRNA gene sequencing. All the isolates succeeded in the gastrointestinal transit tolerance assay and successively colonized mucosal epithelial cells. Based on the results of these in vitro assays, both P. acidilactici and E. faecium can be considered as potential probiotic candidates.

Keywords

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