The Korean Journal of Pain

  • 제33권4호
  • /
  • Pages.335-343
  • /
  • 2020
  • /
  • 2005-9159(pISSN)
  • /
  • 2093-0569(eISSN)
대한통증학회 (The Korean Pain Society)
권호 표지
DOI QR코드

DOI QR Code

Effect of Zhongyi paste on inflammatory pain in mice by regulation of the extracellular regulated protein kinases 1/2-cyclooxygenase-2-prostaglandin E2 pathway

  • Xiao, Ailan (The First Department of Orthopaedic Surgery, Chongqing Traditional Chinese Medicine Hospital) ;
  • Wu, Chuncao (Office of Academic Research, Chongqing Traditional Chinese Medicine Hospital) ;
  • Kuang, Lei (The First Department of Orthopaedic Surgery, Chongqing Traditional Chinese Medicine Hospital) ;
  • Lu, Weizhong (The First Department of Orthopaedic Surgery, Chongqing Traditional Chinese Medicine Hospital) ;
  • Zhao, Xin (Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education) ;
  • Kuang, Zhiping (The First Department of Orthopaedic Surgery, Chongqing Traditional Chinese Medicine Hospital) ;
  • Hao, Na (The First Department of Orthopaedic Surgery, Chongqing Traditional Chinese Medicine Hospital)
  • 투고 : 2020.06.25
  • 심사 : 2020.08.19
  • 발행 : 2020.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Zhongyi paste is a traditional Chinese medicine herbal paste that is externally applied to reduce inflammation and relieve pain. Methods: An acute foot swelling inflammation model in C57BL/6J mice was established by carrageenan-induced pathogenesis. Zhongyi paste raised the pain threshold and also reduced the degree of swelling in mice with carrageenan-induced foot swelling. Results: Analysis indicated that serum tumor necrosis factor-alpha, interleukin-1 beta, and prostaglandin E2 (PGE2) cytokine levels and PGE2 levels in the paw tissue of the mice were decreased by Zhongyi paste treatment. The quantitative polymerase chain reaction and western blot results showed that Zhongyi paste downregulated the mRNA and protein expression of extracellular signal-regulated kinase 1/2 (ERK1/2), and cyclooxygenase-2 (COX-2), and also downregulated the mRNA expression of PGE2. At the same time, the Zhongyi paste exerted a stronger effect as an external drug than that of indomethacin, which is an oral drug, and voltaren, which is an externally applied drug. Conclusions: Our results indicated that Zhongyi paste is a very effective drug to reduce inflammatory swelling of the foot, and its mechanism of action is related to regulation of the ERK1/2-COX-2-PGE2 pathway.

키워드

참고문헌

  1. Bundgaard L, Sorensen MA, Nilsson T, Salling E, Jacobsen S. Evaluation of systemic and local inflammatory parameters and manifestations of pain in an equine experimental wound model. J Equine Vet Sci 2018; 68: 81-7. https://doi.org/10.1016/j.jevs.2018.05.219
  2. McCormack K. The spinal actions of nonsteroidal antiinflammatory drugs and the dissociation between their antiinflammatory and analgesic effects. Drugs 1994; 47 Suppl 5: 28-45. https://doi.org/10.2165/00003495-199400475-00006
  3. DeHaven-Hudkins DL. Peripherally restricted opioid drugs: advances and retreats. Curr Opin Anaesthesiol 2003; 16: 541-5. https://doi.org/10.1097/00001503-200310000-00016
  4. Abramson SB. Treatment of gout and crystal arthropathies and uses and mechanisms of action of nonsteroidal antiinflammatory drugs. Curr Opin Rheumatol 1992; 4: 295-300. https://doi.org/10.1097/00002281-199206000-00002
  5. Graham DM, McPherson H, Lieberman P. Skin testing in the evaluation of hymenoptera allergy and drug allergy. Immunol Allergy Clin North Am 2001; 21: 301-20. https://doi.org/10.1016/S0889-8561(05)70208-3
  6. Li YJ, Zhou J, Lei M. Clinical therapeutic study on the treatment of knee osteoarthritis with Zhongyi paste. Asia-Pac Tradit Med 2018; 8: 167-9.
  7. Schrier DJ, Moniot S, Gluckman MI, Gilbertsen RB. The topical anti-inflammatory effects of a topical preparation of meclofenamic acid on carrageenan-induced footpad swelling in mice. J Pharm Pharmacol 1987; 39: 57-9. https://doi.org/10.1111/j.2042-7158.1987.tb07165.x
  8. Singh G, Katyal SL, Brown WE, Kennedy AL, Singh U, Wong-Chong ML. Clara cell 10 kDa protein (CC10): comparison of structure and function to uteroglobin. Biochim Biophys Acta 1990; 1039: 348-55. https://doi.org/10.1016/0167-4838(90)90270-P
  9. Li YY, Huang SS, Lee MM, Deng JS, Huang GJ. Anti-inflammatory activities of cardamonin from Alpinia katsumadai through heme oxygenase-1 induction and inhibition of $NF-{\kappa}B$ and MAPK signaling pathway in the carrageenaninduced paw edema. Int Immunopharmacol 2015; 25: 332-9. https://doi.org/10.1016/j.intimp.2015.02.002
  10. Minghetti L, Polazzi E, Nicolini A, Creminon C, Levi G. Upregulation of cyclooxygenase-2 expression in cultured microglia by prostaglandin E2, cyclic AMP and non-steroidal anti-inflammatory drugs. Eur J Neurosci 1997; 9: 934-40. https://doi.org/10.1111/j.1460-9568.1997.tb01444.x
  11. Martey CA, Pollock SJ, Turner CK, O'Reilly KM, Baglole CJ, Phipps RP, et al. Cigarette smoke induces cyclooxygenase-2 and microsomal prostaglandin E2 synthase in human lung fibroblasts: implications for lung inflammation and cancer. Am J Physiol Lung Cell Mol Physiol 2004; 287: L981-91. https://doi.org/10.1152/ajplung.00239.2003
  12. Li W, Wu J, Yang Y, Pan L. Oxysophocarpine ameliorates carrageenan-induced inflammatory pain via adjusting ERK1/2-COX-2-PGE2 pathway in mice. Animal Hus Feed Sci 2017; 38: 15-9.
  13. Wang L, Deng T, Zhang J, Chen Y. Resveratrol ameliorates inflammatory pain via inhibiting the COX-2/PGE2 signaling pathway in mice. Pharm Clin Chinese Mater Med 2017; 33: 35-8.
  14. Liu Y, Zhang YJ, Yu Y, Li H, Liu L. Study on antipyretic, analgesic and anti-inflammatory effects of Fangfeng Jiere mixture. J Emerg Tradit Chinese Med 2019; 28: 1576-9.
  15. Yang H, Cheng X, Wang YH, Du GH. Effects of esculin on the swelling of toes and inflammation induced by carrageenan in mice. Chinese J New Drugs 2016; 25: 2319-22.
  16. Said R, Abdel-Rehim M, Sadeghi B, Al-Hashemi S, Hassan Z, Hassan M. Cyclophosphamide pharmacokinetics in mice: a comparison between retro orbital sampling versus serial tail vein bleeding. Open Pharmacol J 2007; 1: 30-35. https://doi.org/10.2174/1874143600701010030
  17. Liu B, Ma R, Zhang J, Sun P, Yi R, Zhao X. Preventive effect of small-leaved Kuding tea (Ligustrum robustum (Roxb.) Bl.) polyphenols on D-galactose-induced oxidative stress and aging in mice. Evid Based Complement Alternat Med 2019; 2019: 3152324.
  18. Wang M, Wang J, Tan R, Wu Q, Qiu H, Yang J, et al. Effect of berberine on PPAR ${\alpha}$ /NO activation in high glucose- and insulin-induced cardiomyocyte hypertrophy. Evid Based Complement Alternat Med 2013; 2013: 285489.
  19. Chou TC, Chang LP, Li CY, Wong CS, Yang SP. The antiinflammatory and analgesic effects of baicalin in carrageenanevoked thermal hyperalgesia. Anesth Analg 2003; 97: 1724-9. https://doi.org/10.1213/01.ANE.0000087066.71572.3F
  20. Zhao Y, Yao Y, Zhang H, Wu C, Deng Z, Wang S. Pharmacokinetics and correlation between in vivo and in vitro transdermal penetration of indomethacin submicron emulsion gel. Chin J Mod Appl Pharm 2019; 36: 31-5.
  21. Hu G, Zhang L, Xu B, Ma F, Wang K, Lu Y, et al. Clinical study on diclofenac diethylamine emulsion delivery via nano plum-blossom needle for treatment of acute gouty arthritis. Chinese J Inform Tradit Chinese Med 2020; 27: 8-11.
  22. Castro-Lopes JM, Tavares I, Tolle TR, Coimbra A. Carrageenan-induced inflammation of the hind foot provokes a rise of GABA-immunoreactive cells in the rat spinal cord that is prevented by peripheral neurectomy or neonatal capsaicin treatment. Pain 1994; 56: 193-201. https://doi.org/10.1016/0304-3959(94)90094-9
  23. Kalyan Kumar G, Dhamotharan R, Kulkarni NM, Mahat MY, Gunasekaran J, Ashfaque M. Embelin reduces cutaneous TNF-${\alpha}$ level and ameliorates skin edema in acute and chronic model of skin inflammation in mice. Eur J Pharmacol 2011; 662: 63-9. https://doi.org/10.1016/j.ejphar.2011.04.037
  24. Holmin S, Mathiesen T. Intracerebral administration of interleukin-1beta and induction of inflammation, apoptosis, and vasogenic edema. J Neurosurg 2000; 92: 108-20. https://doi.org/10.3171/jns.2000.92.1.0108
  25. Fang JQ, Liu F, Shao XM, Wu YY. [Effect of electroacupuncture on carrageenan-induced inflammation, IL-1beta and TNF-alpha concentrations and their mRNA expressions in toe tissue in rats]. Zhen Ci Yan Jiu 2007; 32: 224-8. Chinese.
  26. Zhang M, Zhou J, Wang L, Li B, Guo J, Guan X, et al. Caffeic acid reduces cutaneous tumor necrosis factor alpha (TNF-${\alpha}$), IL-6 and IL-$1{\beta}$ levels and ameliorates skin edema in acute and chronic model of cutaneous inflammation in mice. Biol Pharm Bull 2014; 37: 347-54. https://doi.org/10.1248/bpb.b13-00459
  27. Zambelli VO, Picolo G, Fernandes CAH, Fontes MRM, Cury Y. Secreted phospholipases $A_{2}$ from animal venoms in pain and analgesia. Toxins (Basel) 2017; 9: 406. https://doi.org/10.3390/toxins9120406
  28. Stachowska E, Dolegowska B, Dziedziejko V, Rybicka M, Kaczmarczyk M, Bober J, et al. Prostaglandin E2 (PGE2) and thromboxane A2 (TXA2) synthesis is regulated by conjugated linoleic acids (CLA) in human macrophages. J Physiol Pharmacol 2009; 60: 77-85.
  29. Lavigne P, Shi Q, Jolicoeur FC, Pelletier JP, Martel-Pelletier J, Fernandes JC. Modulation of IL-1beta, IL-6, TNF-alpha and PGE(2) by pharmacological agents in explants of membranes from failed total hip replacement. Osteoarthritis Cartilage 2002; 10: 898-904. https://doi.org/10.1053/joca.2002.0846
  30. Damlar I, Esen E, Tatli U. Effects of glucosamine-chondroitin combination on synovial fluid IL-$1{\beta}$, IL-6, TNF-${\alpha}$ and PGE2 levels in internal derangements of temporomandibular joint. Med Oral Patol Oral Cir Bucal 2015; 20: e278-83.
  31. Tipton DA, Flynn JC, Stein SH, Dabbous MKh. Cyclooxygenase-2 inhibitors decrease interleukin-1beta-stimulated prostaglandin E2 and IL-6 production by human gingival fibroblasts. J Periodontol 2003; 74: 1754-63. https://doi.org/10.1902/jop.2003.74.12.1754
  32. Ciruela A, Dixon AK, Bramwell S, Gonzalez MI, Pinnock RD, Lee K. Identification of MEK1 as a novel target for the treatment of neuropathic pain. Br J Pharmacol 2003; 138: 751-6. https://doi.org/10.1038/sj.bjp.0705103
  33. Li T, Hu J, Du S, Chen Y, Wang S, Wu Q. ERK1/2/COX-2/PGE2 signaling pathway mediates GPR91-dependent VEGF release in streptozotocin-induced diabetes. Mol Vis 2014; 20: 1109-21.
  34. Lo HM, Chen CL, Tsai YJ, Wu PH, Wu WB. Thrombin induces cyclooxygenase-2 expression and prostaglandin E2 release via PAR1 activation and ERK1/2- and p38 MAPK-dependent pathway in murine macrophages. J Cell Biochem 2009; 108: 1143-52. https://doi.org/10.1002/jcb.22341