DOI QR코드

DOI QR Code

Improving Effects of Brassica oleracea L. var. italica Sprout Extract on Alcoholic Liver Dysfunction

  • Received : 2019.12.26
  • Accepted : 2020.05.07
  • Published : 2020.06.01

Abstract

Alcoholic fatty liver disorder has become a frequent health concern worldwide. To investigate the effects of Brassica oleracea (B. oleracea) sprout extract (BOE), the present study was designed with alcoholic fatty liver in the rat. Initially, the effects of BOE on liver parameters were examined. Male rats were divided into five groups. The normal control group was fed the normal diet, and the BOE group was fed the high fat diet and ethanol with/without BOE for 4 weeks. After 4 weeks feeding period, rats were sacrificed and their livers and blood were used for fatty liver-related biomarkers analyses. As a result, BOE ameliorated fatty liver-related enzymes profiles in liver tissues and also reduced blood alcohol concentration in rat model. We demonstrated that BOE protected the high fat diet and alcohol-induced fatty liver in rat model. Furthermore, BOE increased detoxificative abilities against alcohol.

Keywords

References

  1. Baek, S.H., M. Park, J.H. Suh and H.S. Choi. 2008. Protective effects of an extract of young radish (Raphanus sativus L.) cultivated with sulfur (sulfur-radish extract) and of sulforaphane on carbon tetrachloride-induced hepatotoxicity. Biosci. Biotechnol. Biochem. 72:1176-1182. https://doi.org/10.1271/bbb.7054510
  2. Blanco, A. and G. Blanco. 2017. Chapter 27 - Vitamins. Medical Biochemistry. Academic Press, MA (USA). pp. 645-687.
  3. Bones, A.M and J.T. Rossiter. 1996. The myrosinaseglucosinolate system, its organisation and biochemistry. Physiol. Plant. 97:194-208. https://doi.org/10.1034/j.1399-3054.1996.970128.x
  4. Chen, Y.J., A.D. Myracle, M.A. Wallig and E.H. Jeffery. 2016. Dietary broccoli protects against fatty liver development but not against progression of liver cancer in mice pretreated with diethylnitrosamine. J. Func. Foods 24:57-62. https://doi.org/10.1016/j.jff.2016.03.028
  5. Fahey, J.W., Y. Zhang and P. Talalay. 1997. Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc. Natl. Acad. Sci. U.S.A. 94:10367-10372. https://doi.org/10.1073/pnas.94.19.10367
  6. Kikuchi, M., Y. Ushida, H. Shiozawa, R. Umeda, K. Tsuruya, Y. Aoki, H. Suganuma and Y. Nishizaki. 2015. Sulforaphanerich broccoli sprout extract improves hepatic abnormalities in male subjects. World J. Gastroenterol. 21:12457-67. https://doi.org/10.3748/wjg.v21.i43.12457
  7. Kleiner, D.E., E.M. Brunt, M. Van Natta, C. Behling, M.J. Contos, O.W. Cummings, L.D. Ferrell, Y.C. Liu, M.S. Torbenson, A. Unalp-Arida, M. Yeh, A.J. McCullough, A.J. Sanyal and Nonalcoholic steatohepatitis clinical research network. 2005. Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology 41:1313-1321. https://doi.org/10.1002/hep.20701
  8. Klomparens, E.A. and Y. Ding 2019. The neuroprotective mechanisms and effects of sulforaphane. Brain. Circ. 5:74-83. https://doi.org/10.4103/bc.bc_7_19
  9. Lai, R.H., A.S. Keck, M.A. Wallig, L.G. West and E.H. Jeffery. 2008. Evaluation of the safety and bioactivity of purified and semi-purified glucoraphanin. Food. Chem. Toxicol. 46:195-202. https://doi.org/10.1016/j.fct.2007.07.015
  10. Lei, P., W. Zhao, B. Pang, X. Yang, B.L. Li, M. Ren and Y.J. Shan. 2018. Broccoli sprout extract alleviates alcoholinduced oxidative stress and endoplasmic reticulum stress in C57BL/6 mice. J. Agri. Food Chem. 66:5574-5580. https://doi.org/10.1021/acs.jafc.8b01653
  11. Mokhtari, R.B., N. Baluch, T.S. Homayouni, E. Morgatskaya, S. Kumar, P. Kazemi and H. Yeger. 2018. The role of sulforaphane in cancer chemoprevention and health benefits: A mini-review. J. Cell Commun. Signal 12:91-101. https://doi.org/10.1007/s12079-017-0401-y
  12. Oh, H.G., J.-H. Kim, E.H. Shin, Y.R. Kang, B.G. Lee, S.H. Park, D.I. Moon, L.S. Kwon, Y.P. Kim, M.H. Choi, O.J. Kim, K.H. Park and H.Y. Lee. 2013. Improving effects of Platycodon extracts jelly on ${\beta}$-amyloid-induced cytotoxicity and scopolamine-induced cognitive impairment animal models. Korean J. Plant Res. 26:417-425. https://doi.org/10.7732/kjpr.2013.26.4.417
  13. Ravichandiran, P., S.A. Subramaniyan, S.Y. Kim, J.S. Kim, B.H. Park, K.S. Shim and D.J. Yoo. 2019. Synthesis and anticancer evaluation of 1,4-naphthoquinone derivatives containing a phenylaminosulfanyl moiety. Chem. Med. Chem. 14:532-544. https://doi.org/10.1002/cmdc.201800749
  14. Shehatou, G.S. and G.M. Suddek. 2016. Sulforaphane attenuates the development of atherosclerosis and improves endothelial dysfunction in hypercholesterolemic rabbits. Exp. Biol. Med. (Maywood). 241:426-436. https://doi.org/10.1177/1535370215609695
  15. Sun, C.C., S.J. Li, C.L. Yang, R.L. Xue, Y.Y. Xi, L. Wang, Q. L. Zhao and D.J. Li. 2015. Sulforaphane attenuates muscle inflammation in dystrophin-deficient mdx mice via NF-E2-related factor 2 (Nrf2)-mediated inhibition of NF-${\kappa}$B signaling pathway. J. Biol. Chem. 290:17784-17795. https://doi.org/10.1074/jbc.M115.655019
  16. Teng, W., Y. Li, M. Du, X. Lei, S. Xie and F. Ren. 2019. Sulforaphane prevents hepatic insulin resistance by blocking serine palmitoyltransferase 3-mediated ceramide biosynthesis. Nutrients 11:1185. https://doi.org/10.3390/nu11051185
  17. Wang, A.J., Z. Yang, V. Grinchuk, A. Smith, B. Qin, N. Lu, D. Wang, H. Wang, T.R. Ramalingam, T.A. Wynn, J.F. Urban Jr, T. Shea-Donohue and A. Zhao. 2015. IL-25 or IL-17E protects against high-fat diet-induced hepatic steatosis in mice dependent upon IL-13 activation of STAT6. J. Immunol. 195:4771-80. https://doi.org/10.4049/jimmunol.1500337
  18. Xie, G.H., S.I. Jeong, S.E. Choi, J.J. Jeong and K.H. Park. 2017. Ca2+-ATPase and cAMP-mediated anti-apoptotic effects of Acanthopanax senticosus extracts on ischemia/reperfusion liver damages. Korean J. Plant Res. 30:647-653 https://doi.org/10.7732/KJPR.2017.30.6.647
  19. Xie, G.H., S.E. Choi, M.J. Mun, J.J. Jeong and K.H. Park. 2018. Ameliorative effects of combinative injection of Ginko biloba leaves extract and vitamin C on ischemia/reperfusion liver damages model. Korean J. Plant Res. 31:268-273. https://doi.org/10.7732/KJPR.2018.31.3.268
  20. Xu, D., M. Xu, S. Jeong, Y. Qian, H. Wu, Q. Xia and X. Kong. 2019. The role of Nrf2 in liver disease: Novel molecular mechanisms and therapeutic approaches. Front. Pharmacol. 9:1428. https://doi.org/10.3389/fphar.2018.01428
  21. Yoshida, K., Y. Ushida, T. Ishijima, H. Suganuma, T. Inakuma, N. Yajima, K. Abe and Y. Nakai. 2015. Broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury. World J. Gastroenterol. 21:10091-10103. https://doi.org/10.3748/wjg.v21.i35.10091
  22. Zhang, Y., R. Munday, H.E. Jobson, C.M. Munday, C. Lister, P. Wilson, J.W. Fahey and P. Mhawech-Fauceglia. 2006. Induction of GST and NQO1 in cultured bladder cells and in the urinary bladders of rats by an extract of broccoli (Brassica oleracea var. italica) sprouts. J. Agric. Food Chem. 54:9370-9376. https://doi.org/10.1021/jf062109h
  23. Zhang, Y. and P. Talalay. 1998. Mechanism of differential potencies of isothiocyanates as inducers of anticarcinogenic phase 2 enzymes. Cancer Res. 58:4632-4639.