Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Effects of Portulaca oleracea L. Supplementation on Reproductive Performance, Blood Profiles, Immune Response and Fecal Microflora in Multiparous Sows

모돈 사료 내 Portulaca oleracea L.의 첨가가 번식성적, 혈액성상, 면역반응 및 분 중 미생물에 미치는 영향

  • Oh, Seung-Min (Gyeongsangbuk-do Livestock Research Institute) ;
  • Choi, Yo-Han (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Hyun-Jung (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jeon, Se-Min (Adbiotech, co., Ltd.) ;
  • Kim, Jin-Soo (Department of Animal Industry Convergence, College of Animal Life Sciences, Kangwon National University)
  • 오승민 (경상북도 축산기술연구소) ;
  • 최요한 (농촌진흥청 국립축산과학원 양돈과) ;
  • 정현정 (농촌진흥청 국립축산과학원 양돈과) ;
  • 전세민 ((주)애드바이오텍) ;
  • 김진수 (강원대학교 동물생명과학대학 동물산업융합학과)
  • Received : 2020.03.25
  • Accepted : 2020.07.03
  • Published : 2020.07.31
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Abstract

This study was undertaken to determine the effects of dietary supplementation with herbal extracts of Portulaca oleracea L. (POL), on the reproductive performance, blood profiles, immune response, and fecal microbial population in multiparous sows. On the basis of initial body weight, a total of 20 multiparous sows (Landrace×Yorkshire; 229.2±1.15 kg) were randomly allotted to 2 treatment groups, each including 10 replicates. The dietary treatments were administered during the lactation period, and included a corn-soybean meal-based diet (control group) and diet supplemented with 0.05% POL. Throughout the experimental period, no significant differences were observed between the two diet groups for body weight, backfat thickness, and reproductive performance. Blood profile analysis revealed significantly decreased lymphocyte concentrations and IL-1β levels (p<0.05), and increased serum IgG levels (p=0.051), of sows consuming the POL supplemented diet. Furthermore, coliform counts tended to decrease in sows consuming diet supplemented with POL (p=0.063). Taken together, our results indicate that POL supplemented diets exert beneficial effects on blood profiles, immune response, and fecal microflora of multiparous sows, without any negative effect on the reproductive performance.

본 실험은 모돈 사료 내 Portulaca oleracea L.(POL)의 첨가가 번식성적, 혈액성상, 면역반응 및 분 중 미생물에 미치는 영향을 구명하기 위해 수행하였다. 본 실험을 위해 총 20두의 모돈(Landrace×Yorkshire; 229.2±1.15 kg)을 공시하였으며, 개시체중에 기반하여, 2처리 10반복, 반복당 1두씩 완전임의 배치하였다. 실험 처리구는 옥수수-대두박 기초사료를 대조구로 하여 기초사료 내 POL을 0.05% 첨가한 처리구가 포함되었으며, 분만 직후부터 포유기간 동안 수행하였다. 사료 내 POL을 첨가하였을 때, 모돈의 체중, 등지방두께 및 번식성적에서는 유의적인 차이가 나타나지 않았다(p>0.05). 혈액성상에서 사료 내 POL의 첨가하였을 때, 모돈의 림프구가 유의적으로 감소하는 것으로 나타났다(p<0.05). 또한, 면역반응에서 사료 내 POL을 첨가하였을 때 포유모돈의 혈 중 IL-1β의 수치는 유의적으로 감소하였으며(p<0.05), IgG는 증가하는 경향이 관찰되었다(p=0.051). 분 중 미생물에서는 POL 처리구에서 포유모돈의 coliforms이 감소하는 경향을 확인하였다(p=0.063). 본 실험의 결과들을 종합해보면, 사료 내 POL의 첨가는 번식성적에 부정적인 영향을 미치지 않으며, 모돈의 혈액성상, 면역반응 및 분 중 미생물에 긍정적인 효과를 미치는 것을 시사한다.

Keywords

References

  1. C. Y. Lee, K. H. Baik, D. H. Lee, H. C. Park, "Relationships of plasma insulin-like growth factor (IGF)-I and IGF-II concentrations to litter size and lactation performance in landrace and yorkshire pigs", Journal of Animal Science and Technology, Vol.45, No.1, pp.33-40, 2003. DOI: https://doi.org/10.5187/JAST.2003.45.1.033
  2. H. J. Kim, J. H. Cho, Y. J. Chen, J. S. Yoo, S. O. Shin, Y. Huang, I. H. Kim, "Effects of dietary coconut fat powder supplementation on performance and milk characteristics in lactating sow", Journal of Animal Science and Technology, Vol.49, No.6, pp.773-782, 2007. DOI: https://doi.org/10.5187/JAST.2007.49.6.773
  3. R. C. Thaler, J. L. Nelssen, R. D. Goodband, G. L. Allee, "Effect of dietary folic acid supplementation on sow performance through two parities", Journal of Animal Science and Technology, Vol.67, No.12, pp.3360-3369, 1988. DOI: https://doi.org/10.2527/jas1989.67123360x
  4. Y. X. Yang, S. Heo, Z. Jin, J. H. Yun, J. Y. Choi, S. Y. Yoon, M. S. Park, B. K. Yang, B. J. Chae, "Effects of lysine intake during late gestation and lactation on blood metabolites, hormones, milk composition and reproductive performance in primiparous and multiparous sows", Animal Reproduction Science, Vol.112, pp.199-214. 2008. DOI: https://doi.org/10.1016/j.anireprosci.2008.04.031
  5. K. H. Kim, A. Hosseindoust, S. L. Ingale, S. H. Lee, H. S. Noh, Y. H. Choi, S. M. Jeon, Y. H. Kim, B. J. Chae, "Effects of gestational housing on reproductive performance and behavior of sows with different backfat thickness", Asian-Australasian Journal of Animal Sciences, Vol.29, No.1, pp.142-148, 2016. DOI: http://dx.doi.org/10.5713/ajas.14.0973
  6. J. W. Hong, I. H. Kim, O. S. Kwon, S. H. Lee, J. W. Kim, "Effects of Yolk Antibodies to Replace Antibiotic in Segregated Early-Weaned Pigs", Journal of Animal Science and Technology, Vol.43, No.2, pp.177-184, 2001.
  7. S. K. Lim, J. E. Lee, H. S. Lee, H. M. Nam, D. C. Moon, G. C. Jang, Y. J. Park, Y. G. Jung, S. C. Jung, S. H. Wee, "Trends in antimicrobial scales for livestock and fisheries in korea during 2003-2012", Korean Journal of Veterinary Research, Vol.54, No.2, pp.81-86, 2014. DOI: http://dx.doi.org/10.14405/kjvr.2014.54.2.81
  8. P. A. Thacker, "Alternatives to antibiotics as growth promoters for use in swine production: a review", Journal of Animal Science and Biotechnology, Vol.4, No.35, 2013. DOI: https://doi.org/10.1186/2049-1891-4-35
  9. N. K. Im, G. S. Jeong, "Neuroprotective effect of ethyl acetate fraction of Portulaca oleracea L.", Korea Journal of Pharmaconosy, Vol.44, No.4, pp.379-383, 2013.
  10. L. Xiang, D. Xing, W. Wang, R. Wang, Y. Ding, L. Du, "Alkaloids from Portulaca oleracea L.", Phytochemistry, Vol.66, No.21, pp.2595-2601, 2005. DOI: https://doi.org/10.1016/j.phytochem.2005.08.011
  11. N. E. Awad, "Lipid content and antimicrobial activity of phenolic constituents of cultivated Portulaca oleracea L.", Bulletin of Faculty of Pharmacy, Cairo University, Vol.32, No.1, pp.137-142, 1994.
  12. N. Sakai, K. Inada, M. Okamoto, Y. Shizuri, Y. Fukuyama, "Portuloside A, a monoterpene glucoside from Portulaca oleracea ", Phytochemistry, Vol.42, No.6, pp.1625-1628, 1996. DOI: https://doi.org/10.1016/0031-9422(96)00202-6
  13. C. Y. Li, Y. H. Meng, Z. M. Ying, N. Xu, D. Hao, M. Z. Gao, W. J. Zhang, L. Xu, Y. C. Gao, X. X. Ying, "Three Novel Alkaloids from Portulaca oleracea L. and Their Antiinflammatory Effects", Journal of Agricultural and Food chemistry, Vol.64, pp.5837-5844, 2016. DOI: https://doi.org/10.1021/acs.jafc.6b02673
  14. M. Iranshahy, B. Javadi, M. Iranshahi, S. P. Jahanbakhsh, S. Mahyari, F. V. Hassani, G. Karimi, "A review of traditional uses, phytochemistry and pharmacology of Portulaca oleracea L.", Journal of ethnopharmacology, Vol. 205, pp.158-172, 2017. DOI: http://dx.doi.org/10.1016/j.jep.2017.05.004
  15. L. Wang, L. Gong, L. Zhu, C. Peng, J. Liao, L. Ke, B. Dong, "Effects of activated charcoal-herb extractum complex on the growth performance, immunological indices, intestinal morphology and microflora in weaning piglets", RSC advances, Vol.9, No.11, pp.5948-5957, 2019. DOI: https://doi.org/10.1039/C8RA10283J
  16. L. Wang, L. Zhu, L. Gong, X. Zhang, Y. Wang, J. Liao, L. Ke, B. Dong, "Effects of activated charcoal-herb extractum complex on antioxidant status, lipid metabolites and safety of excess supplementation in weaned piglets", Animals, Vol.9, No.12, pp.1151. 2019. DOI: https://doi.org/10.3390/ani9121151
  17. National Research Council(NRC), "Nutrient requirements of swine", Livestock Production Science, pp.208-238, The national academies press, 2012.
  18. SAS. 2012, SAS Software for PC. Release 9.3, SAS Institute. Ins, Cart, NC, USA.
  19. R. Zabielski, Z. Gajewski, J. L. Valverde Piedra, D. Laubitz, J. Wilczak, B. Balasinska, G. Kulasek, "The perinatal development of the gastrointestinal tract in piglets can be modified by supplementation of sow diet with bioactive substances", Livestock science, Vol.109, No.1-3, pp.34-37, 2007. DOI: https://doi.org/10.1016/j.livsci.2007.01.037
  20. K. H. Kim, K. S. Kim, J. E. Kim, H. J. Jung, S. D. Lee, S. J. Sa, J. K. Hong, T. Y. Hur, J. C. Park, Y. H. Kim, "Effects of dietary supplementation of Codonopsis pilosula extract powder on the productivity and immunity in sows and piglets", Korean Journal of Organic Agriculture, Vol.21, No.3, pp.423-435, 2013. https://doi.org/10.11625/KJOA.2013.21.3.423
  21. L. Calani, M. Dall'Asta, E. Derlindati, F. Scazzina, R. Bruni, D. Del Rio, "Colonic metabolism of polyphenols from coffee, green tea, and hazelnut skins", Journal of Clinical Gastroenterology, Vol.46, pp.S95-S99, 2012. DOI: https://doi.org/10.1097/MCG.0b013e318264e82b
  22. K. Chan, M. W. Islam, M. Kamil, R. Radhakrishnan, M. N. M. Zakaria, M. Habibullah, A. Attas, "The analgesic and anti-inflammatory effects of Portulaca oleracea L. subsp. sativa (Haw.) Celak", Journal of ethnopharmacology, Vol.73, No.3, pp.445-451, 2000. DOI: https://doi.org/10.1016/S0378-8741(00)00318-4
  23. B. Chen, H. Zhou, W. Zhao, W. Zhou, Q. Yuan, G. Yang, "Effects of aqueous extract of Portulaca oleracea L. on oxidative stress and liver, spleen leptin, $PAR{\alpha}$ and FAS mRNA expression in high-fat diet induced mice", Molecular Biology Reports, Vol.39, No.8, pp.7981-7988, 2012. DOI: https://doi.org/10.1007/s11033-012-1644-6
  24. S. D. Upadhaya, J. W. Park, I. H. Kim, "Effect of plant extract YGF251 on growth performance, reproductive performance and insulin like growth factor secretion in primiparous and multiparous sows", Animal Nutrition and Feed Technology, Vol.16, pp. 393-402, 2016. DOI: https://doi.org/10.5958/0974-181X.2016.00035.4
  25. B. J. Min, O. S. Kwon, J. W. Hong, K. S. Son, I. H. Kim, "Effects of herbal plant mixture (Miracle(R)) supplementation on the productivities of lactating sows and growth performance and hematological characteristics of piglets", Journal of Animal Science and Technology, Vol.47, No.3, pp.371-378, 2005. DOI: https://doi.org/10.5187/JAST.2005.47.3.371
  26. E. Pietrzak, E. R. Grela, "The effects of adding lucerne protein concentrate to diets on the reproductive traits and blood metabolic profiles of sows and piglets", Journal of Animal and Feed Science, Vol.24, No.3, pp.216-225, 2015. https://doi.org/10.22358/jafs/65627/2015
  27. M. Kaveh, A. Eidi, A. Nemati, M. H. Boskabady, "The extract of Portulaca oleracea and its constituent, alpha linolenic acid affects serum oxidant levels and inflammatory cells in sensitized rats", Iranian Journal of Allergy Asthma and Immunology, Vol.16, No.3, pp.256-270, 2017.
  28. T. Guzik, R. Korbut, T. Adamek-Guzik, "Nitric oxide and superoxide in inflammation", Journal of physiology pharmacology, Vo.54, No.4, pp.469-487, 2003.
  29. S. W. Seo, "The anti-inflammatory effect of Portulaca oleracea 70% EtOH Extracts on lipopolysaccharide-induced inflammatory response in RAW 264.7 cells", The Korea Journal of Herbology, Vol.30, No.6, pp.33-38, 2015. DOI: https://doi.org/10.6116/kjh.2015.30.6.33.
  30. J. Feng, F. Deng, Y. Yu,, C. Yang, A. Chen, "Effects of ${\alpha}$-carotene microcapsules on reproducibility, immunity and antioxidant capability in late-pregnancy sows", Journal of Zhejiang University (Agriculture and Life Sciences), Vol.35, No.6, pp.665-669, 2009. https://doi.org/10.3785/j.issn.1008-9209.2009.06.012
  31. B. B. Jensen, "The impact of feed additives on the microbial ecology of the gut in young pigs", Journal of Animal and Feed Sciences, Vol.7, No.1, pp.45-64, 1998. DOI: https://doi.org/10.22358/jafs/69955/1998
  32. J. Willamil, I. Badiola, E. Devillard, P. A. Geraert, D. Torrallardona, "Wheat-barley-rye-or corn-fed growing pigs respond differently to dietary supplementation with a carbohydrase complex", Journal of Animal Science, Vol.90, NO.3, pp.824-832, 2012. DOI: https://doi.org/10.2527/jas.2010-3766
  33. H. H. Giang, T. Q. Viet, B. Ogle, J. E. Lindberg, "Effects of supplementation of probiotics on the performance, nutrient digestibility and faecal microflora in growing-finishing pigs", Asian-Australasian Journal of Animal Science, Vol.24, No.5, pp.655-661, 2011. DOI: https://doi.org/10.5713/ajas.2011.10238