Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Tolerance limit of nitrite exposure to hybrid grouper (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂): hematological parameters and plasma components

대왕범바리(Epinephelus fuscoguttatus ♀×E. lanceolatus ♂)의 아질산 급성노출에 따른 내성한계: 혈액성상 및 혈장성분의 변화

  • Cho, Jea-Hwang (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Seok-Ryel (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Hur, Young Baek (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Lee, Kyung Mi (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Jun-Hwan (West Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 조재황 (국립수산과학원 서해수산연구소 양식산업과) ;
  • 김석렬 (국립수산과학원 서해수산연구소 양식산업과) ;
  • 허영백 (국립수산과학원 서해수산연구소 양식산업과) ;
  • 이경미 (국립수산과학원 서해수산연구소 양식산업과) ;
  • 김준환 (국립수산과학원 서해수산연구소 양식산업과)
  • Received : 2019.12.27
  • Accepted : 2020.02.18
  • Published : 2020.03.31
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Abstract

Hybrid grouper (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂) (mean weight 27.3±3.8 g, mean length 11.6±0.7 cm) were exposed to waterborne nitrite at 0, 100, 200, 400, 800, and 1600mg L-1 for 96 hours. The hematocrit and hemoglobin values were significantly decreased by exposure to 100 mg L-1 and 400 mg L-1, respectively. In plasma components, no significant change was observed in magnesium. Glucose was significantly increased by 200 and 400 mg L-1 nitrite but reduced by 800 mg L-1. Cholesterol was significantly decreased by 400 mg L-1 nitrite, but there was no significant change in total protein. GPT(glutamic pyruvate transaminase) was significantly increased by exposure to 200 and 400mg L-1. ALP(Alkaline phosphatase) was significantly increased by 800 mg L-1. The results of this study indicate that acute exposure to nitrite changes physiological parameters, such as hematological properties and plasma components.

본 실험에서 아질산 급성노출은 대왕범바리의 혈액학적 성상 및 혈장성분에 유의적 변화를 나타내었다. 혈액학적 성상인 Hct와 Hb는 아질산 노출에 의한 유의적 감소를 확인하였다. 혈장 성분인 glucose, cholesterol, GPT 및 ALP는 아질산 노출에 의해 유의적으로 변화를 나타냄을 확인하였다. 본 실험의 결과 아질산 노출 100mg L-1 이상의 농도는 대왕범바리의 혈액 성상 및 혈장 성분의 유의적 영향을 미치며, 800 mg L-1의 아질산 급성 노출은 대량 폐사를 유발할 수 있으나, 기존 국내 양식 대상종인 Olive flounder, P. Olivaceus는 171.043 mg L-1 (Kim et al. 2018), Yellow tail, Seriola quinqueradiata는 147 mg L-1 (Sugiyama et al. 1991)에 비해 상대적으로 높은 값을 보여 아질산 내성이 상대적으로 강함을 확인할 수 있었다.

Keywords

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