융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
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동과자 추출물의 항산화, 항염 및 미백 효능

Anti-oxidant, anti-inflammatory and Whitening effect of Benincasa hispida seed extract

  • 박규리 (서경대학교 일반대학원 미용예술학과) ;
  • 이지안 (서경대학교 일반대학원 미용예술학과)
  • Park, Gyu-Ri (Dept. of Beauty Art, Graduate School, Seokyeong University) ;
  • Lee, Ji-An (Dept. of Beauty Art, Graduate School, Seokyeong University)
  • 투고 : 2020.05.11
  • 심사 : 2020.07.20
  • 발행 : 2020.07.28
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초록

본 연구는 피부 관리에 사용되는 천연화장품 원료로서 동과자 추출물의 활용 가능성을 조사하기 위해 항산화, 항염 그리고 미백 효능을 평가하였다. DPPH 자유라디컬 소거 활성은 농도 의존적으로 감소하였으며, 총폴리페놀 함량은 메탄올추출물(22.42±0.002 mgGAE/g)이 열수추출물(9.77±0.002 mgGAE/g)보다 더 높게 나타났다. MTT assay 결과 동과자 추출물에 의한 RAW264.7 세포와 B16F10 세포에서의 세포독성은 없었다. 또한 LPS 처리에 의해 활성화된 RAW264.7 세포에서 NO 생성과 TNF-α 분비 억제능을 확인하였다. B16F10 melanoma 세포에서 동과자 추출물은 α-MSH에 의한 멜라닌 합성을 유의하게 저해하는 것으로 나타났다. 게다가, westernblot 분석결과 동과자 메탄올추출물은 MITF, TRP-1 및 TRP-2 단백질의 발현 수준을 현저하게 감소시켰다. 이상의 결과로 동과자 추출물은 항산화, 항염 그리고 미백 효능을 가진 기능성 화장품 소재로써의 가치가 높을 것으로 사료된다.

To investigate the natural cosmetic ingredients of Benincasa hispida seed extract on skin care, we measured anti-oxidant and anti-inflammatory, and whitening effect. DPPH free radical scavenging activity was increased in a dose-dependent manner. The total phenolic content was higher in methanol extract (22.42±0.002 mgGAE/g) than water extract (9.77±0.002 mgGAE/g). MTT assay was demonstrated that the seed extract did not have a cytotoxic effect in RAW264.7 and B16F10 cell lines. We also examined to find out the inhibitory activity on NO production and secretion of TNF-α cytokine in LPS-induced RAW264.7 cells. In B16F10 melanoma cells, the seed extract significantly suppressed α-MSH induced melanin synthesis. Furthermore, westernblot analysis revealed that methanol extract dramatically downregulated the expression level of MITF, TRP-1 and TRP-2. Taken together, the B. hispida seed extract posses anti-oxidant, anti-inflammatory and skin whitening activities, which might provided its functional efficacy in cosmetic materials.

키워드

참고문헌

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