Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Cryotop and ReproCarreir products for cryopreservation of bovine blastocysts through survival rate and blastocysts quality

  • Kim, Jin-Woo (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Yang, Seul-Gi (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Park, Hyo-Jin (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Kim, Ju Hwan (Modu Science) ;
  • Lee, Dong-Mok (Biomedical Manufacturing Technology Center, Korea Institute of Industrial Technology) ;
  • Woo, Seong-Min (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Kim, Hyun-Jeong (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Kim, Hyun Ah (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Jeong, Jae-Hoon (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Lee, Min Ji (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
  • Received : 2020.05.25
  • Accepted : 2020.06.12
  • Published : 2020.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Cryopreservation is used for blastocyst preservation of most mammalian embryos and is an important technique for breeding. We aimed to compare the efficiency of the cryopreservation method using the standard Cryotop device and the ReproCarrier device, a domestic product manufactured in Korea. The efficacy of the two devices was analyzed based on the survival rate, intracellular levels of reactive oxygen species (ROS), and apoptosis of the vitrified bovine blastocysts. The survival rates of the vitrified-warmed blastocysts were similar between the ReproCarrier group (58.4 ± 17.7%) and Cryotop group (59.9 ± 14.1%). Intracellular ROS levels and apoptotic index were determined by DCFDA staining and TUNEL assay. Changes in intracellular ROS levels, number of total nuclei, and cellular apoptosis of vitrified blastocysts after cryopreservation were not significantly different between the two groups. These results indicate that the ReproCarrier device method is as effective as the standard Cryotop method for vitrification of bovine blastocysts in vitro.

Keywords

References

  1. Ansari SM, Saquib Q, Attia SM, Abdel-Salam EM, Alwathnani HA, Faisal M, Alatar AA, Al-Khedhairy AA, Musarrat J. 2018. Pendimethalin induces oxidative stress, DNA damage, and mitochondrial dysfunction to trigger apoptosis in human lymphocytes and rat bone-marrow cells. Histochem. Cell Biol. 149:127-141. https://doi.org/10.1007/s00418-017-1622-0
  2. Choi J, Park SM, Lee E, Kim JH, Jeong YI, Lee JY, Park SW, Kim HS, Hossein MS, Jeong YW, Kim S, Hyun SH, Hwang WS. 2008. Anti-apoptotic effect of melatonin on preimplantation development of porcine parthenogenetic embryos. Mol. Reprod. Dev. 75:1127-1135. https://doi.org/10.1002/mrd.20861
  3. Dos Santos-Neto PC, Cuadro F, Barrera N, Crispo M, Menchaca A. 2017. Embryo survival and birth rate after minimum volume vitrification or slow freezing of in vivo and in vitro produced ovine embryos. Cryobiology 78:8-14. https://doi.org/10.1016/j.cryobiol.2017.08.002
  4. He Q, Zhou X, Liu Y, Gou W, Cui J, Li Z, Wu Y, Zuo D. 2018. Titanium dioxide nanoparticles induce mouse hippocampal neuron apoptosis via oxidative stress- and calcium imbalance-mediated endoplasmic reticulum stress. Environ. Toxicol. Pharmacol. 63:6-15. https://doi.org/10.1016/j.etap.2018.08.003
  5. Inaba Y, Miyashita S, Somfai T, Geshi M, Matoba S, Dochi O, Nagai T. 2016. Cryopreservation method affects DNA fragmentation in trophectoderm and the speed of re-expansion in bovine blastocysts. Cryobiology 72:86-92. https://doi.org/10.1016/j.cryobiol.2016.03.006
  6. Lee AR, Hong K, Choi SH, Park C, Park JK, Lee JI, Bang JI, Seol DW, Lee JE, Lee DR. 2019. Anti-apoptotic regulation contributes to the successful nuclear reprogramming using cryopreserved oocytes. Stem Cell Reports 12:545-556. https://doi.org/10.1016/j.stemcr.2019.01.019
  7. Min SH, Song BS, Yeon JY, Kim JW, Bae JH, Park SY, Lee YH, Chang KT, Koo DB. 2014. A cathepsin B inhibitor, E-64, improves the preimplantation development of bovine somatic cell nuclear transfer embryos. J. Reprod. Dev. 60:21-27. https://doi.org/10.1262/jrd.2013-074
  8. Moulavi F, Soto-Rodriguez S, Kuwayama M, Asadi-Moghaddam B, Hosseini SM. 2019. Survival, re-expansion, and pregnancy outcome following vitrification of dromedary camel cloned blastocysts: a possible role of vitrification in improving clone pregnancy rate by weeding out poor competent embryos. Cryobiology 90:75‐82. https://doi.org/10.1016/j.cryobiol.2019.08.002
  9. Mucci N, Aller J, Kaiser GG, Hozbor F, Cabodevila J, Alberio RH. 2006. Effect of estrous cow serum during bovine embryo culture on blastocyst development and cryotolerance after slow freezing or vitrification. Theriogenology 65:1551-1562. https://doi.org/10.1016/j.theriogenology.2005.08.020
  10. Nanassy L, Lee K, Javor A, Machaty Z. 2008. Effects of activation methods and culture conditions on development of parthenogenetic porcine embryos. Anim. Reprod. Sci. 104:264-274. https://doi.org/10.1016/j.anireprosci.2007.01.019
  11. Park SY, Kim EY, Cui XS, Tae JC, Lee WD, Kim NH, Park SP, Lim JH. 2006. Increase in DNA fragmentation and apoptosis-related gene expression in frozen-thawed bovine blastocysts. Zygote 14:125-131. https://doi.org/10.1017/s0967199406003649
  12. Paschoal DM, Sudano MJ, Schwarz KRL, Maziero RRD, Guastali MD, Crocomo LF, Magalhaes LCO, Martins Jr. A, Leal CLV, Landim-Alvarenga FDC. 2017. Cell apoptosis and lipid content of in vitro-produced, vitrified bovine embryos treated with forskolin. Theriogenology 87:108-114. https://doi.org/10.1016/j.theriogenology.2016.08.011
  13. Pereira BA, Zangeronimo MG, Castillo-Martin M, Gadani B, Chaves BR, Rodriguez-Gil JE, Bonet S, Yeste M. 2019. Supplementing maturation medium with insulin growth factor I and vitrification-warming solutions with reduced glutathione enhances survival rates and development ability of in vitro matured vitrified-warmed pig oocytes. Front. Physiol. 9:1894. https://doi.org/10.3389/fphys.2018.01894
  14. Takahashi M. 2012. Oxidative stress and redox regulation on in vitro development of mammalian embryos. J. Reprod. Dev. 58:1-9. https://doi.org/10.1262/jrd.11-138N
  15. Yang SG, Park HJ, Kim JW, Jung JM, Kim MJ, Jegal HG, Kim IS, Kang MJ, Wee G, Yang HY, Lee YH, Seo JH, Kim SU, Koo DB. 2018. Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos. Sci. Rep. 8:10130. https://doi.org/10.1038/s41598-018-28497-5