Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Improvement of cadmium tolerance and accumulation of Phragmites spp. Tabarka by ethyl methane sulfonate mutagenesis

  • Kim, Young-Nam (Department of Environmental Horticulture, University of Seoul) ;
  • Kim, Jiseong (Department of Environmental Horticulture, University of Seoul) ;
  • Lee, Jeongeun (Department of Environmental Horticulture, University of Seoul) ;
  • Kim, Sujung (Department of Environmental Horticulture, University of Seoul) ;
  • Lee, Keum-Ah (Department of Environmental Horticulture, University of Seoul) ;
  • Kim, Sun-Hyung (Department of Environmental Horticulture, University of Seoul)
  • Received : 2020.12.18
  • Accepted : 2020.12.22
  • Published : 2020.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M2 mutant reeds under Cd stress conditions (up to 1,500 µM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.

Keywords

References

  1. Ali H, Khan E, Ilahi I (2019) Environmental chemistry and ecotoxicology of hazardous heavy metals: environmental persistence, toxicity, and bioaccumulation. J Chem 2019: ID6730305
  2. Ali NA, Bernal MP, Ater M (2004) Tolerance and bioaccumulation of cadmium by Phragmites australis grown in the presence of elevated concentrations of cadmium, copper, and zinc. Aquat Bot 80:163-176
  3. Arthur EL, Rice PJ, Rice PJ, Anderson TA, Baladi SM (2005) Phytoremediation-an overview. Crit Rev Plant Sci 24:109-122
  4. Benavides MP, Gallego SM, Tomaro ML (2005) Cadmium toxicity in plants. Braz J Plant Physiol 17(1):21-34
  5. Chen J, Huang JW, Caspar T, Cunningham SD (1997) Arabidopsis thaliana as a model system for studying lead accumulation and tolerance in plants. In EL Kruger, TA Anderson, JR Coats, eds, Phytoremediation of soil and water contaminants. American Chemical Society, Washington. p 264
  6. Cherian S, Oliveira MM (2005) Transgenic Plants in Phytoremediation: Recent Advance ad New Possibilities. Environ Sci Technol 39(24):9377-9390
  7. Cresser MS, Parsons JW (1979) Sulfuric-perchloric acid digestion of plant material for the determination of nitrogen, phosphorus, potassium, calcium and magnesium. Anal Chem Acta 109:431-436
  8. Das N, Bhattacharya S, Maiti MK (2016) Enhanced cadmium accumulation and tolerance in transgenic tobacco overexpressing rice metal tolerance protein gene OsMTP1 is promising for phytoremediation. Plant Physiol Biochem 105:297-309
  9. Das P, Samantaray S, Rout GR (1997) Studies on cadmium toxicity in plants: a review. Environ Pollut 98:29-36
  10. Ederli L, Reale L, Ferranti F, Pasqualini S (2004) Response induced by high concentration of cadmium in Phragmites australis roots. Physiol Plant 121:66-74
  11. Gunarathne V, Mayakaduwa S, Ashiq A, Weerakoon SR, Biswas JK, Vithanage M (2019) Transgenic plants: benefits, applications, and potential risks in phytoremediation. Transgenic Plant Technology for Remediation of Toxic Metals and Metalloids. Elsevier, U.K. pp 89-102
  12. Hill MK (2010) Understanding environmental pollution. Cambridge University Press, Cambridge
  13. Higuchi K, Kanai M, Tsuchiya M, Ishii H, Shibuya N, Fujita N, Nakamura Y, Suzui N, Fujimaki S, Miwa E (2015) Common reed accumulates starch in its stem by metabolic adaptation under Cd stress conditions. Front Plant Sci 6:138
  14. Kim KH, Kim YN, Lim GH, Lee MN, Jung YH (2011a) Expression of catalase (CAT) and ascorbate peroxidase (APX) in MuS1 transgenic tobacco under cadmium stress. Korean J Soil Fert 44(1):53-57
  15. Kim YN, Kim JS, Seo SG, Lee Y, Baek SW, Kim IS, Yoon HS, Kim KR, Kim SH, Kim KH (2011) Cadmium resistance in tobacco plants expressing the MuSI gene. Plant Biotechnol Rep 5:323-329
  16. Maple J, Moller SG (2007) Mutagenesis in Arabidopsis. In E Rosato, ed, Circadian Rhythms. Humana Press. pp 197-206
  17. Mantovi P, Marmiroli M, Maestri E, Tagliavini S, Piccinini S, Marmiroli N (2003) Application of a horizontal subsurface flow constructed wetland on treatment of dairy parlor wastewater. Bioresource Technol 88:85-94
  18. Negri MC (1996) Plants that remove contaminants from the environment. Lab Med 27:36-9
  19. Nehnevajova E, Lyubenova L, Herzig R, Schroder P, Schwitzguebel JP, Schmulling T (2012) Metal accumulation and response of antioxidant enzymes in seedlings and adult sunflower mutants with improved metal removal traits on a metal-contaminated soil. Environ Exp Botany 76:39-48
  20. Phang IC, Taylor HH, Leung DWM (2012) Chemical mutagenesis for improving potential of plants to remediate environments with heavy metal contaminants. Curr Top Med Chem 11:47-52
  21. Poonawala IS, Jana MM, Nadgauda NS (1999) Factors influencing bud break and mass-scale micropropagation of three Phragmites species: P. karka, P. communis, and P. australis. Plant Cell Rep18:696-700
  22. Stoltz E, Greger M (2002) Accumulation properties of As, Cd, Cu, Pb and Zn by four wetland plant species growing on submerged mine tailings. Environ Exp Bot 47:271-280
  23. Toppi LS, Gabbrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41(2):105-130
  24. Ullah S, Khan J, Hayat K., Abdelfattah Elateeq A, Salam U, Yu B, Ma Y, Wang H, Tang ZH (2020) Comparative study of growth, cadmium accumulation and tolerance of three chickpea (Cicer arietinum L.) cultivars. Plants 9(3):310