대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제46권2호
  • /
  • Pages.185-194
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  • 2020
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  • 1226-2587(pISSN)
  • /
  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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반응표면분석을 이용한 진생베리의 활성 성분 최적 추출 조건에 관한 연구

Study of Optimized Simultaneous Extraction Conditions for Active Component of Ginseng Berry using Response Surface Methodology

  • 고희경 (충북대학교 공업화학과) ;
  • 박준성 (충북대학교 공업화학과)
  • Go, Hee Kyoung (Department of Engineering Chemistry, Chungbuk National University, College of Engineering) ;
  • Park, Junseong (Department of Engineering Chemistry, Chungbuk National University, College of Engineering)
  • 투고 : 2020.06.11
  • 심사 : 2020.06.26
  • 발행 : 2020.06.30
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초록

본 연구에서는 진생베리로부터 활성 성분인 ginsenoside Re와 Rb1의 함량과 항산화 활성이 높은 추출물을 얻기 위한 최적의 추출조건을 확인하였다. 70% 에탄올과 증류수를 추출 용매로 하여 교반, 초음파 및 마이크로파를 이용하여 추출한 후 ginsenoside Re와 Rb1 의 함량, 총 폴리페놀 함량, 항산화 활성 및 친환경적 제조 방법 여부를 고려한 결과 증류수 용매의 마이크로파 추출법이 가장 적합한 추출방법인 것을 확인하였다. 마이크로파 추출법의 최적 추출조건을 설정하기 위해 반응표면분석법(RSM)을 사용하여 마이크로파 출력량(50 ~ 200 W, X1), 용매와 진생베리의 비율(5 ~ 20 times, X2), 추출시간(30 ~ 120 s, X3)을 독립변수로 최적화 실험을 진행한 결과 도출된 회귀방정식의 결정계수는 0.9보다 높으며 p-value값은 0.05보다 작아 모델의 적합성이 확인되었다. Ginsenoside와 총 폴리페놀 함량을 위한 최적 추출조건은 마이크로파 출력량(200 W), 진생베리와 추출 용매 비율(20 times), 추출시간(90 s)로 확인되었다. 최적 추출조건에서 총 폴리페놀 함량이 6.23 mg GAE/g, ginsenoside Re 17.69 mg/g, Rb1 16.01 mg/g으로 예측되었고, 실제 실측검증에서 각각 6.33 mg GAE/g, 17.79 mg/g, 15.59 mg/g으로 예측된 값에 대하여 유사한 결과를 확인할 수 있었다.

This study was conducted to find out the optimal extraction conditions to obtain extracts with a high content of ginsenosides and antioxidant activity using the ginseng berry. After extraction by stirring, ultrasound and microwave method using 70% ethanol and distilled water as solvents, the results of considering the content of ginsenoside Re and Rb1, total polyphenol content, antioxidant activity, and whether it is an environmentally friendly manufacturing method, it was confirmed that the microwave method using distilled water is good method of extraction. The optimization of extraction conditions for microwave method were made by response surface methodology (RSM). Microwave power (50 ~ 200 W, X1), solvent and ginseng berry ratio (5 ~ 20 times, X2) and the extraction time (30 ~ 120 s, X3) were used as independent variables. The model showed a good fit having a determination coefficient of the regression equation of 0.9 or more and a p-value less than 0.05. Estimated conditions for the maximized extraction of ginsenoside contents and total polyphenols were 200 w in microwave power, 20 times in solvent and ginseng berry ratio, and 90 s in extraction time. Predicted values at the optimum conditions were total polyphenols of 6.23 mg GAE/g, ginsenoside Re of 17.69 mg/g, and ginsenoside Rb1 of 16.01 mg/g. In the verification of the actual measurement the obtained values showed 6.33 mg GAE/g, 17.79 mg/g, and 15.59 mg/g, respectively, in good agreement with predicted values.

키워드

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