한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제48권2호
  • /
  • Pages.138-147
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  • 2020
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Characterization of Yeast and Bacterial Type Strains with Food and Agricultural Applications by MALDI-TOF Mass Spectrometry Biotyping

  • Harnpicharnchai, Piyanun (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency) ;
  • Jaresitthikunchai, Janthima (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency) ;
  • Seesang, Mintra (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency) ;
  • Jindamorakot, Sasitorn (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency) ;
  • Tanapongpipat, Sutipa (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency) ;
  • Ingsriswang, Supawadee (National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency)
  • 투고 : 2019.10.16
  • 심사 : 2020.02.13
  • 발행 : 2020.06.28
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초록

Various microorganisms play important roles in food fermentation, food spoilage, and agriculture. In this study, the biotype of 54 yeast and bacterial strains having high potential for utilization in food and agriculture, including Candida spp., Lactobacillus spp., and Acetobacter spp., were characterized by matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS). This characterization using a fast and robust method provides much-needed information on the selected microorganisms and will facilitate effective usage of these strains in various applications. Importantly, the unique protein profile of each microbial species obtained from this study was used to create a database of fingerprints from these species. The database was validated using microbial strains of the same species by comparing the mass spectra with the created database through pattern matching. The created reference database provides crucial information and is useful for further utilization of a large number of valuable microorganisms relevant to food and agriculture.

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참고문헌

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