Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Smilax guianensis Vitman Extract Prevents LPS-Induced Inflammation by Inhibiting the NF-κB Pathway in RAW 264.7 Cells

  • Kim, Ju Gyeong (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Min Jeong (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Lee, Ji Su (Division of Bioengineering, Incheon National University) ;
  • Sydara, Kongmany (Ministry of Health, Institute of Traditional Medicine) ;
  • Lee, Sangwoo (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Byun, Sanguine (Department of Biotechnology, Yonsei University) ;
  • Jung, Sung Keun (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2019.11.19
  • Accepted : 2020.03.22
  • Published : 2020.06.28
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Abstract

Nutraceutical treatments can reduce inflammation and prevent the development of inflammatory diseases. In this study, the anti-inflammatory effects of Smilax guianensis Vitman extract (SGE) were examined. SGE suppressed lipopolysaccharide (LPS)-mediated nitrite production in RAW 264.7 cells. SGE also prevented the LPS-induced expression of inducible nitric oxide synthase (iNOS) but not cyclooxygenase (COX)-2. Western blot analysis showed that SGE attenuated LPS-induced phosphorylation of IκB kinase (IKK), inhibitor of kappa B (IκB), and p65. Additionally, SGE inhibited LPS-induced IκB degradation in RAW 264.7 cells. Western blot analysis of the cytosolic and nuclear fractions, as well as immunofluorescence assay results, revealed that SGE suppressed LPS-induced p65 nuclear translocation in RAW 264.7 cells. Moreover, SGE reduced LPS-induced interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) mRNA expression and IL-1β and IL-6 protein expression in RAW 264.7 cells. Collectively, these results indicate that SGE suppresses the NF-κB signaling pathway and thereby inhibits the production of NO, IL-1β, and IL-6.

Keywords

References

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