Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory Effects of Andrographis Herba MeOH Extract on LPS-induced Raw 264.7 Cells

천심련(穿心蓮) 메탄올 추출물의 항염증효과

  • Jung, Ji Yun (College of Korean Medicine, Daegu Haany University) ;
  • Park, Chung A (College of Korean Medicine, Daegu Haany University)
  • 정지윤 (대구한의대학교 한의과대학) ;
  • 박정아 (대구한의대학교 한의과대학)
  • Received : 2020.05.01
  • Accepted : 2020.05.25
  • Published : 2020.05.31
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Abstract

PURPOSE : Andrographis Herba is used as a traditional herbal medicine in the Asian countries for the treatment common cold, fever, diabetes, hypertension, hepatitis, skin infections, snake bite, and other diseases. In this study, we investigated the anti-inflammatory effect of MeOH extract of Andrographis Herba (AHME) on LPS-activated Raw 264.7 cells. METHODS : Cell viability was determined by MTT assay. Nitric oxide (NO) production was determined by Griess reagent. Pro-inflammatory cytokines were detected by enzyme-linked immunosorbent assay. Expression levels of pro-inflammatory proteins were determined by Western blot analysis. RESULTS : Production of NO in LPS activated Raw 264.7 cells, was significantly decreased by pre-treatment with 3-30 ㎍/mL of AHME. Production of pro-inflammatory mediators such as TNF-α and IL were significantly decreased by AHME 30 ㎍/mL pre-treatment. AHME significantly decreased p-IκB and NF-κB expression. CONCLUSION : The results of this study indicate that AHME could inhibit the acute inflammatory response, via modulation of NF-κB activation.

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References

  1. Jiangsuxinyixueyuan. Zhonhyaodacidian. Shanghai: Shanghaikexuejizhu Press. 1979:1728-39.
  2. Guo LZ. Modern Practical Herbology. Beijing: Renminweisheng Press. 2000:186-88.
  3. Hossain MS, Urbi Z, Sule A, Hafizur Rahman KM. Andrographis paniculata (Burm. f.) Wall. ex Nees: a review of ethnobotany, phytochemistry, and pharmacology. Scientific World Journal. 2014;2014:274905.
  4. Rekik A. Muluye, Yuhong Bian, Paulos N. Alemu. Anti-inflammatory and Antimicrobial Effects of Heat-Clearing Chinese Herbs: A Current Review. J Tradit Complement Med. 2014;4(2):93-8 https://doi.org/10.4103/2225-4110.126635
  5. Dua VK, Ojha VP, Roy R, Joshi BC. Valecha N, Devi CU. Anti-malarial activity of some xanthone isolated from the roots of Andrographis paniculata. J Ethnophamacol. 2004;95(2-3):247-51. https://doi.org/10.1016/j.jep.2004.07.008
  6. Korean Society of Pathologists. Pathology (3ed). Seoul:Komoonsa. 1998:65-8.
  7. Kubes P. Inducible nitric oxide synthase: a little bit of good in all of us. Gut. 2000;47:6-9. https://doi.org/10.1136/gut.47.1.6
  8. Wink DA, Mitchell JB. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med. 1998;25:434-56. https://doi.org/10.1016/S0891-5849(98)00092-6
  9. Alderton WK, Cooper CE, Knowles RG. Nitric oxide synthases: structure, function and inhibition. Biochem J. 2001;357:593-615. https://doi.org/10.1042/bj3570593
  10. Sharifi AM, Mousavi SH, Bakhshayesh M, Tehrani FK, Mahmoudian M, Oryan S. Study of correlation between lead-induced cytotoxicity and nitric oxide production in PC12 cells. Toxicol Lett. 2005;160(1):43-8. https://doi.org/10.1016/j.toxlet.2005.06.008
  11. Park EY, Park SJ, Lee JR, Jee SY, Byun SH, Kim SC. Cytoprotective effects of Liquiritigenin, a component of Licorice, against Lead-induced cytotoxicity in PC-12 cells. Kor, J. Herbology. 2007;22(2):17-24.
  12. Sheeja K, Shihab PK, Kuttan G. Antioxidant and anti-inflammatory activities of the plant Andrographis paniculata Nees. Immunopharmacol Immunotoxicol. 2006;28(1):129-40. https://doi.org/10.1080/08923970600626007
  13. Aktan F. iNOS-mediated nitric oxide production and its regulation. Life Sciences. 2004;75:639-53. https://doi.org/10.1016/j.lfs.2003.10.042
  14. Aktan F, Henness S, Roufogalis BD, Ammit AJ. Gypenosides derived from Gynostemma pentaphyllum suppress NO synthesis in murine macrophages by inhibiting iNOS enzymatic activity and attenuating NF-kappaB- mediated iNOS protein expression. Nitric Oxide. 2003;8(4):235-42. https://doi.org/10.1016/S1089-8603(03)00032-6
  15. Chen F, Castranova V, Shi X. New insights into the role of nuclear factor-kappaB in cell growth regulation. Am J Pathol. 2001;159(2):387-97. https://doi.org/10.1016/S0002-9440(10)61708-7
  16. Jirik FR, Podor TJ, Hirano T, Kishimoto T, Loskutoff DJ, Carson DA, Lotz M. Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells. J Immunol. 1989;142(1):144-7.
  17. Rose-John S. Interleukin-6 Family Cytokines. Cold Spring Harb Perspect Biol. 2018;10:a028415 https://doi.org/10.1101/cshperspect.a028415
  18. Zhang Q, Lenardo MJ, Baltimore D. 30 Years of $NF-{\kappa}B$: A Blossoming of Relevance to Human Pathobiology. Cell. 2017;168(1-2):37-57. https://doi.org/10.1016/j.cell.2016.12.012
  19. Mitchell S, Vargas J, Hoffmann A. Signaling via the $NF{\kappa}B$ system. Wiley Interdiscip Rev Syst Biol Med. 2016;8(3):227-41. https://doi.org/10.1002/wsbm.1331
  20. Korbecki J, Bajdak-Rusinek K. The effect of palmitic acid on inflammatory response in macrophages: an overview of molecular mechanisms. Inflamm Res. 2019;68(11):915-32. https://doi.org/10.1007/s00011-019-01273-5
  21. Hoesel B, Schmid JA. The complexity of $NF-{\kappa}B$ signaling in inflammation and cancer. Mol Cancer. 2013;12:86. https://doi.org/10.1186/1476-4598-12-86
  22. Sun SC. The non-canonical $NF-{\kappa}B$ pathway in immunity and inflammation. Nat Rev Immunol. 2017;17(9):545-58. https://doi.org/10.1038/nri.2017.52
  23. Manavalan B, Basith S, Choi YM, Lee G, Choi S. Structure-function relationship of cytoplasmic and nuclear $I{\kappa}B$ proteins: an in silico analysis. PLoS One. 2010;5(12):e15782. https://doi.org/10.1371/journal.pone.0015782
  24. Fernandez G, Zaikos TD, Khan SZ, Jacobi AM, Behlke MA, Zeichner SL. Targeting $I{\kappa}B$ proteins for HIV latency activation: the role of individual $I{\kappa}B$ and $NF-{\kappa}B$ proteins. J Virol. 2013;87(7):3966-78. https://doi.org/10.1128/JVI.03251-12
  25. Zou W, Xiao Z, Wen X, Luo J, Chen S, Cheng Z, Xiang D, Hu J, He J. The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the $NF-{\kappa}B$ pathway. BMC Complement Altern Med. 2016;16(1):483. https://doi.org/10.1186/s12906-016-1466-5