Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Laxative Activity of the Hot-Water Extract Mixture of Hovenia dulcis Thunb. and Phyllostachys pubescens Mazel in Chronic Constipation Model SD Rats

  • Oh, Kyo-Nyeo (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Kim, Yujin (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Choi, Eun Jin (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Lee, Hyunmi (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Hong, Ji Ae (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Kim, Miri (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Oh, Dool-Ri (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Jung, Myung-A (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Park, Ro-Dong (Agroceuticals Lab, Haenam Natural Farming Association Corporation) ;
  • Kim, Seong-il (Agroceuticals Lab, Haenam Natural Farming Association Corporation) ;
  • Yong, Ju-seon (Agroceuticals Lab, Haenam Natural Farming Association Corporation) ;
  • Lee, Hui-Seop (Agroceuticals Lab, Haenam Natural Farming Association Corporation) ;
  • Ban, SangOh (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research) ;
  • Choi, Chul-yung (Jeonnam Bioindustry Foundation, Jeonnam Institute of Natural Resources Research)
  • Received : 2019.11.22
  • Accepted : 2020.02.18
  • Published : 2020.05.28
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Abstract

This study examined the laxative effects of hot-water extracts of Hovenia dulcis Thunb. (HD), Phyllostachys pubescens Mazel (PM), and a 2:8 mixture of both (HP) in two chronic constipation models. For the loperamide-induced constipation model, animals were divided into an untreated group, negative control group (loperamide 4 mg/kg), positive control group (bisacodyl 4 mg/kg) group, and six treatment groups (HP 100 or 400, HD 50 or 100, and PM 100 or 400 mg/kg). For the low-fiber diet-induced constipation model, animals were divided into an untreated group (normal diet), negative control group (low-fiber diet), positive control group (Agio granule, 620 mg/kg), and the same treatment groups. Fecal number, weight, fecal water content, and intestinal transit ratio were higher in the groups treated with HP, HD, and PM than in the groups treated with loperamide or low-fiber diet. Thickness of colon mucosa and muscle layers were increased in the treated groups. Colon tension increased in the HP groups, and [Ca2+]i measurements using fura-2 as an indicator showed that HP inhibits ATP-mediated Ca2+ influx in IEC-18 cells. These results showed that the HP mixture has laxative activity by increased mucin secretion and inducing contractile activity and relaxation. It may be a useful therapeutic strategy for ameliorating in chronic constipation.

Keywords

References

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