한국양봉학회지 (Journal of Apiculture)

한국양봉학회 (The Apicultural Society of Korea)
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국내 양봉농가에서 채취한 정제봉독(Apis mellifera L.)의 지방세포 분화 억제 효과

Inhibitory Effect of Purified Bee Venom(Apis mellifera L.) on Adipogenesis in Korea

  • 한상미 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김효영 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 우순옥 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김세건 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 최홍민 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 문효정 (농촌진흥청 국립농업과학원 농업생물부)
  • Han, Sang Mi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Hyo Young (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Woo, Soon Ok (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Se Gun (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Choi, Hong Min (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Moon, Hyo Jung (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
  • 투고 : 2020.03.20
  • 심사 : 2020.04.20
  • 발행 : 2020.04.30
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 서양종꿀벌(Apis mellifera L.) 일벌에서 봉독채집장치를 사용하여 채집한 후 정제한 정제봉독(purified bee venom)의 항비만 효과를 검정하기 위하여 지방전구세포인 3T3-L1 세포에서 지방분화에 미치는 영향을 조사하였다. 정제봉독은 1 mg/mL 이하의 농도로 처리했을 경우에는 3T3-L1 세포에서 세포독성을 유발하지 않는 것으로 확인되었다. 정제봉독을 3T3-L1 세포에 처리한 후 지방분화를 Oil-red-O 염색약으로 염색하여 비교하여 보았을 때, 정제봉독은 무처리구에 비교하여 낮은 지방축적률을 나타내어 지방세포 분화를 억제하는 것으로 확인되었다. 또한 정제봉독은 지방 분화 특이 전사인자인 C/EBPα와 PPARγ 유전자 발현을 억제시켰다. 이에 본 연구결과를 바탕으로 정제봉독이 지방세포 분화 억제를 통한 항비만 소재로서 활용 가능성이 있을 것으로 사료된다.

Bee (Apis mellifera L.) venom is used for the treatment of various human diseases due to its known anti-inflammatory and antibacterial properties. This study investigated the effect of purified bee venom (PBV) on adipogenesis in 3T3-L1 preadipocytes. There was no cytotoxicity while cells were treated with PBV by various concentrations. In the PBV treated cells increases in fat storage were inhibited and also confirmed by oil red o staining. To understand the underlying mechanism at the molecular level were examined on the expression of the genes involved in adipogenesis by using real-time PCR. In this cell model, the mRNA level of adipogenic genes such as peroxisome-proliferator-activated receptors gamma (PPARγ) and CAAAT/enhancer binding protein alpha(C/EBPα) were decreased by PAE treatment, comparing with those of control group. Theses results suggest that PBV inhibits adipocyte differentiation in 3T3-L1 cells and can be used as an efficient natural substance to manage anti-obesity.

키워드

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