Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effect of Banggibongnyeongtang on the immunohistological change in LPS-induced depression rats

방기복령탕(防己茯苓湯)이 백서에서 LPS로 유도된 우울증에서 면역 조직학적 변화에 미치는 효과

  • Park, Sung jun (Department of Formulae Pharmacology, College of Korean Medicine, Gachon University) ;
  • Lee, Tae Hee (Department of Formulae Pharmacology, College of Korean Medicine, Gachon University)
  • 박성준 (가천대학교 한의과대학 방제학교실) ;
  • 이태희 (가천대학교 한의과대학 방제학교실)
  • Received : 2020.02.15
  • Accepted : 2020.02.24
  • Published : 2020.02.28
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Abstract

Objective : This study is accomplished in order to investigate the effect of banggibongnyeongtang on the immunohistological change in LPS-induced depression rats to confirm the histological result of the previous behavioral and biochemical effect. Methods : LPS 5 ㎍ was injected to lateral ventricle and experimental groups were administered BBT intraperitoneally. The concentration of 5-HT in the Medial Prefrontal Cortex, Striatum, Hippocampus, Amygdala was measured by ELISA. IL-1β, TNF-α mRNA and BDNF mRNA expression in the hippocampus was examined by RT-PCR. Result : BBT enhanced 5-HT concentration at all part of brain but no significantly difference at medial prefrontal cortex and striatum. LPS+BBT400 group increased 5-HT concentration significantly than LPS group at hippocampus and amygdala (p<0.05). BBT decreased IL-1β mRNA expression dose dependently but only with significantly decrease in LPS+BBT400 group than LPS group's in Hippocampus (p<0.05). But BBT did not decrease TNF-α mRNA expression significantly in Hippocampus. BBT increased the expression of BDNF mRNA at hippocampus and LPS+BBT400 group significantly increased comparing with LPS group does (p<0.05). Conclusion : It is postulated that the anti-depressant effect of BBT can be validated through the anti-inflammatory effect, 5-HT concentration increase, and the neuro-protective effect mediated by BDNF by combining the results of the previous report about the behavioral and biochemical effect.

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