Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Croton hirtus L'Hér Extract Prevents Inflammation in RAW264.7 Macrophages Via Inhibition of NF-κB Signaling Pathway

  • Kim, Min Jeong (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Ju Gyeong (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Sydara, Kong Many (Ministry of Health, Institute of Traditional Medicine) ;
  • Lee, Sang Woo (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung, Sung Keun (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2019.08.23
  • Accepted : 2019.11.11
  • Published : 2020.04.28
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Abstract

Consumption of anti-inflammatory nutraceuticals may help treat or prevent inflammation-related illnesses such as diabetes, cardiovascular disease, and cancer. This study evaluated the effect of Croton hirtus L'Hér extract (CHE) on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and nuclear factor kappa-B (NF-κB) signaling cascades. CHE significantly suppressed LPS-induced NO production and inducible nitric oxide synthase (iNOS) expression in RAW264.7 macrophages, although cyclooxygenase (COX)-2 expression was not affected. CHE also suppressed LPS-induced IκB kinase (IKK), IκB, and p65 phosphorylation in RAW264.7 cells. Western blot and immunofluorescence assays of cytosol and nuclear p65 and the catalytic subunit of NF-κB showed that CHE suppressed LPS-induced p65 translocation from the cytosol to the nucleus. CHE also suppressed LPS-induced Interleukin (IL)-6 and tumor necrosis factor (TNF)-α production in RAW264.7 cells. These results suggest that CHE prevents NO-mediated inflammation by suppressing NF-κB and inflammatory cytokines.

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References

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