DOI QR코드

DOI QR Code

Ultra Fast Real-Time PCR for Detection of Babesia gibsoni as Point of Care Test

  • 투고 : 2020.01.23
  • 심사 : 2020.02.12
  • 발행 : 2020.02.28

초록

Between May and November 2018, babesiosis was examined in 162 bloods samples obtained to an animal hospital in Jeju island for anemia and medical examination. Sixty-two of 162 (38.3%) were positive by PCR. The ultra fast real-time PCR test with blood directly analyzed without DNA extraction showed the same results. Accurate diagnosis, treatment and prognosis of babesiosis should be combined with clinical symptoms, blood tests, the babesia antibody test, and the PCR antigen test. Ultra fast real-time PCR, with these tests, is expected to be a point-of-care testing (POCT) for easy, fast and accurate diagnosis of babesiosis in the veterinary clinic.

키워드

참고문헌

  1. Birkenheuer AJ, Levy MG, Breitschwerdt EB. Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples. J Clin Microbiol 2003; 41: 4172-4177. https://doi.org/10.1128/JCM.41.9.4172-4177.2003
  2. Boozer L, Macintire D. Canine babesiosis. Vet Clin North Am Small Anim Pract 2003; 33: 885-904. https://doi.org/10.1016/S0195-5616(03)00039-1
  3. Boozer L, Macintire D. Babesia gibsoni: an emerging pathogen in dogs. Compend Contin Educ Vet 2005; 27: 33-42.
  4. Chisholm ES, Ruebush I I TK, Sulzer AJ, Hea ly GR. Babesia microti infection in man: evaluation of an indirect immunofluorescent antibody test. Am J Trop Med Hyg 1978; 27: 14-19. https://doi.org/10.4269/ajtmh.1978.27.14
  5. Conrad P, Thomford J, Tamane I, Whiting J, Bosma L, Uno T, Holshuh HJ, Shelly S. Hemolytic anemia caused by Babesia gibsoni infection in dogs. J Am Vet Med Assoc 1991; 199: 601-605.
  6. Eichenberger RM, Stefanic S, Naucke TJ, Sarkunas M, Zamoka s G, G rimm F, Depla zes P. An ELISA for the early diagnosis of acute canine babesiosis detecting circulating antigen of large Babesia spp. Vet Parasitol 2017; 243: 162-168. https://doi.org/10.1016/j.vetpar.2017.06.030
  7. Farwell GE, LeGrand EK, Cobb CC. Clinical observations on Babesia gibsoni and Babesia canis infection in dogs. J Am Vet Med Assoc 1982; 180: 507-511.
  8. Fukumoto S, Suzuki H, Igarashi I, Xuan X. Fetal experimental transplacental Babesia gibsoni infections in dogs. Int J Parasitol 2005; 35: 1031-1035. https://doi.org/10.1016/j.ijpara.2005.03.018
  9. Garcia AT. Piroplasma infection in dogs in northern Spain. Vet Parasitol 2006; 138: 97-102. https://doi.org/10.1016/j.vetpar.2006.01.043
  10. Inokuma H, Yoshizaki Y, Matsumoto K, Okuda M, Onishi T, Nakagome K, Kosugi R, Hirakawa M. Molecular survey of Babesia infection in dogs in Okinawa, Japan. Vet Parasitol 2004; 121: 341-346. https://doi.org/10.1016/j.vetpar.2004.03.012
  11. Irwin PJ, Hutchinson GW. Clinical and pathological findings of Babesia infection in dogs. Aust Vet J 1991; 68: 204-209. https://doi.org/10.1111/j.1751-0813.1991.tb03194.x
  12. Jacobson LS, Clark IA. The pathophysiology of canine babesiosis: new approaches to an old puzzle. J S Afr Vet Assoc 1994; 65: 134-145.
  13. Kjemtrup AM, Kocan AA, Whitworth L, Meinkoth J, Birkenheuer AJ, Cummings J, Boudreaux MK, Stockham SL, Irizarry-Rovira A, Conrad PA. There are at least three genetically distinct small piroplasms from dogs. Int J Parasitol 2000; 30: 1501-1505. https://doi.org/10.1016/S0020-7519(00)00120-X
  14. Lee MJ, Yu DH, Yoon JS, L i YH, Lee JH, Cha e JS, Pa rk J. Epidemiologic and clinical surveys in dogs infected with Babesia gibsoni in South Korea. Vector Borne Zoonotic Dis 2009; 9: 681-686. https://doi.org/10.1089/vbz.2008.0116
  15. Oh ST, Woo HC. Prevalence of Babesia spp. in dogs of Seogwipo-si, Jeju-do, South Korea. Korean J Vet Serv 2009; 67: 467-471.
  16. Shimada Y, Beppu T, Inokuma H, Okuda M, Onishi T. Ixodid tick species recovered from domestic dogs in Japan. Med Vet Entomol 2003; 17: 38-45. https://doi.org/10.1046/j.1365-2915.2003.00403.x
  17. Taboada J, Merchant SR. Babesiosis of companion animals and man. Vet Clin North Am Small Anim Pract 1991; 21: 103-123. https://doi.org/10.1016/S0195-5616(91)50011-5
  18. Uilenberg G, Franssen FFJ, Perrie NM. Three groups of Babesia canis distinguished and a proposal for nomenclature. Vet Q 1989; 11: 33-40. https://doi.org/10.1080/01652176.1989.9694194
  19. Yonamine H, Ichiki H, Hamakawa M, Shimabukuro T, Sugiyama M, Isoda M. Studies on canine babesiosis in Okinawa Island. Jpn J Vet Sci 1984; 46: 511-518. https://doi.org/10.1292/jvms1939.46.511
  20. Zhou J, Zhang G, Nishikawa Y, Fujisaki K, Xuan X. A 38-kDa protein from Babesia gibsoni a nd i ts a ntibody response in an experimentally infected dog. Vet Parasitol 2006; 141: 345-348. https://doi.org/10.1016/j.vetpar.2006.05.025