Journal of Veterinary Science

  • 제21권1호
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  • Pages.9.1-9.15
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  • 2020
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Derivation of endothelial cells from porcine induced pluripotent stem cells by optimized single layer culture system

  • Wei, Renyue (College of Life Science, Northeast Agricultural University) ;
  • Lv, Jiawei (College of Life Science, Northeast Agricultural University) ;
  • Li, Xuechun (College of Life Science, Northeast Agricultural University) ;
  • Li, Yan (College of Life Science, Northeast Agricultural University) ;
  • Xu, Qianqian (College of Life Science, Northeast Agricultural University) ;
  • Jin, Junxue (College of Life Science, Northeast Agricultural University) ;
  • Zhang, Yu (College of Life Science, Northeast Agricultural University) ;
  • Liu, Zhonghua (College of Life Science, Northeast Agricultural University)
  • 투고 : 2019.08.27
  • 심사 : 2019.11.21
  • 발행 : 2020.01.31
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초록

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

키워드

과제정보

We thank Dr. Jiangqiang Wang from Northeast Agricultural University for his valuable discussion during the manuscript preparation. Thanks also to Dr. Yu Zhang for financial support (University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province, UNPYSCT-2016010).

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