Journal of Veterinary Science

  • 제21권1호
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  • Pages.10.1-10.12
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  • 2020
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Assessment of neovascularization during bone healing using contrast-enhanced ultrasonography in a canine tibial osteotomy model: a preliminary study

  • Jeon, Sunghoon (College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University) ;
  • Jang, Jaeyoung (Haemaru Referral Animal Hospital) ;
  • Lee, Gahyun (College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University) ;
  • Park, Seungjo (College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University) ;
  • Lee, Sang-kwon (College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University) ;
  • Kim, Hyunwook (Haemaru Referral Animal Hospital) ;
  • Choi, Jihye (College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University)
  • 투고 : 2019.07.09
  • 심사 : 2019.11.09
  • 발행 : 2020.01.31
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초록

Blood perfusion of skeletal muscle and callus was evaluated using contrast-enhanced ultrasonography (CEUS) in a canine osteotomy model to determine the applicability of CEUS in the assessment of neovascularization during fracture healing and to compare the vascular signals on CEUS between external skeletal fixation and cast-applied dogs. In 6 Beagle dogs, a simple transverse osteotomy was performed at the left tibial shaft and external skeletal fixation (n = 3) or a cast (n = 3) was applied. Radiography, power Doppler ultrasonography (power Doppler), and CEUS were performed until complete union was achieved. On CEUS, vascular changes were quantitatively evaluated by measuring peak intensity (PI) and time to PI in the soft tissue and callus and by counting the vascular signals. Vascular signals from the soft tissue were detected on power Doppler and CEUS on day 2. Significantly more vascular signals were detected by CEUS than by power Doppler. On CEUS, PI in the surrounding soft tissue was markedly increased after the fracture line appeared indistinctively changed on radiography in all dogs. In the cast-applied dogs, vascular signals from the periosteal and endosteal callus were detected on CEUS before mineralized callus was observed on radiography. CEUS was useful in assessing the vascularity of soft tissue and callus, particularly in indirect fracture healing, and provided indications of a normally healing fracture.

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과제정보

This research was supported by the Animal Medical Institute of Chonnam National University and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (NRF-2018R1A2B6006775).

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