Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Specific urinary metabolites in canine mammary gland tumors

  • Valko-Rokytovska, Marcela (Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Kosice) ;
  • Ocenas, Peter (Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Kosice) ;
  • Salayova, Aneta (Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Kosice) ;
  • Titkova, Radka (Section of Surgery, Orthopaedics, Roentgenology and Reproduction, Small Animal Clinic, University Veterinary Hospital, University of Veterinary Medicine and Pharmacy in Kosice) ;
  • Kostecka, Zuzana (Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Kosice)
  • Received : 2019.07.23
  • Accepted : 2019.11.30
  • Published : 2020.03.31
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Abstract

The identification of biomarkers that distinguish diseased from healthy individuals is of great interest in human and veterinary fields. In this research area, a metabolomic approach and its related statistical analyses can be useful for biomarker determination and allow non-invasive discrimination of healthy volunteers from breast cancer patients. In this study, we focused on the most common canine neoplasm, mammary gland tumor, and herein, we describe a simple method using ultra-high-performance liquid chromatography to determine the levels of tyrosine and its metabolites (epinephrine, 3,4-dihydroxy-L-phenylalanine, 3,4-dihydroxyphenylacetic acid, and vanillylmandelic acid), tryptophan and its metabolites (5-hydroxyindolacetic acid, indoxyl sulfate, serotonin, and kynurenic acid) in canine mammary cancer urine samples. Our results indicated significantly increased concentrations of three tryptophan metabolites, 5-hydroxyindolacetic acid (p < 0.001), serotonin, indoxyl sulfate (p < 0.01), and kynurenic acid (p < 0.05), and 2 tyrosine metabolites, 3,4-dihydroxy-L-phenylalanine (p < 0.001), and epinephrine (p < 0.05) in urine samples from the mammary gland tumor group compared to concentrations in urine samples from the healthy group. The results indicate that select urinary tyrosine and tryptophan metabolites may be useful as non-invasive diagnostic markers as well as in developing a therapeutic strategy for canine mammary gland tumors.

Keywords

Acknowledgement

Realization of this study was supported by the Internal Grant Agency - IGA UVLF 05/2017, Urinary biochemical profile of cancer diseases with potential use in diagnostic practice, Kosice, Slovakia.

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