Journal of Veterinary Science

  • 제21권3호
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  • Pages.46.1-46.18
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  • 2020
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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PM2.5 in poultry houses synergizes with Pseudomonas aeruginosa to aggravate lung inflammation in mice through the NF-κB pathway

  • Li, Meng (College of Life Science, Ludong University) ;
  • Wei, Xiuli (Shandong Provincial Key Laboratory of Quality Safty Monitoring and Risk Assessment for Animal Products) ;
  • Li, Youzhi (Shandong Provincial Key Laboratory of Quality Safty Monitoring and Risk Assessment for Animal Products) ;
  • Feng, Tao (Shandong Provincial Key Laboratory of Quality Safty Monitoring and Risk Assessment for Animal Products) ;
  • Jiang, Linlin (College of Life Science, Ludong University) ;
  • Zhu, Hongwei (College of Life Science, Ludong University) ;
  • Yu, Xin (College of Life Science, Ludong University) ;
  • Tang, Jinxiu (College of Life Science, Ludong University) ;
  • Chen, Guozhong (College of Life Science, Ludong University) ;
  • Zhang, Jianlong (College of Life Science, Ludong University) ;
  • Zhang, Xingxiao (College of Life Science, Ludong University)
  • 투고 : 2019.12.13
  • 심사 : 2020.03.23
  • 발행 : 2020.05.31
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초록

Background: High concentrations of particulate matter less than 2.5 ㎛ in diameter (PM2.5) in poultry houses is an important cause of respiratory disease in animals and humans. Pseudomonas aeruginosa is an opportunistic pathogen that can induce severe respiratory disease in animals under stress or with abnormal immune functions. When excessively high concentrations of PM2.5 in poultry houses damage the respiratory system and impair host immunity, secondary infections with P. aeruginosa can occur and produce a more intense inflammatory response, resulting in more severe lung injury. Objectives: In this study, we focused on the synergistic induction of inflammatory injury in the respiratory system and the related molecular mechanisms induced by PM2.5 and P. aeruginosa in poultry houses. Methods: High-throughput 16S rDNA sequence analysis was used for characterizing the bacterial diversity and relative abundance of the PM2.5 samples, and the effects of PM2.5 and P. aeruginosa stimulation on inflammation were detected by in vitro and in vivo. Results: Sequencing results indicated that the PM2.5 in poultry houses contained a high abundance of potentially pathogenic genera, such as Pseudomonas (2.94%). The lung tissues of mice had more significant pathological damage when co-stimulated by PM2.5 and P. aeruginosa, and it can increase the expression levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α through nuclear factor (NF)-κB pathway in vivo and in vitro. Conclusions: The results confirmed that poultry house PM2.5 in combination with P. aeruginosa could aggravate the inflammatory response and cause more severe respiratory system injuries through a process closely related to the activation of the NF-κB pathway.

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과제정보

This research was financially supported by the National Key Research and Development Program of China (Grant No. 2018YFD0501402), the Key Research and Development Plan of Shandong Province (No. 2017NC210009), the Major Agricultural Applied Technological Innovation Projects of Shandong Province, the Key Research and Development Plan of Yantai (No. 2018XSCC045), and the Innovation Team Project for Modern Agricultural Industrious Technology System of Shandong Province (SDAIT-11-10).

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