Journal of Veterinary Science

  • 제21권3호
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  • Pages.48.1-48.18
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  • 2020
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Generation of cynomolgus monkey fetuses with intracytoplasmic sperm injection based on the MII-stage oocytes acquired by personalized superovulation protocol

  • Huang, Zhangqiong (Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College) ;
  • Li, Yun (Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College) ;
  • Jiang, Qinfang (Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College) ;
  • Wang, Yixuan (Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College) ;
  • Ma, Kaili (Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College) ;
  • Li, Qihan (Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College)
  • 투고 : 2019.09.27
  • 심사 : 2020.03.02
  • 발행 : 2020.05.31
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초록

Background: Mature oocytes at the metaphase II status (MII-stage oocytes) played an important role in assisted reproductive technology in non-human primates. Objectives: In order to improve the proportion of MII-stage oocytes retrieval, three different superovulation protocols were performed on 24 female cynomolgus monkeys. Methods: All the monkeys received once-daily injection of follicle-stimulating hormone (25 international unit [IU]) on day 3 of the menstruation, 3-day intervals, twice daily for 8-12 days until the time of human chorionic gonadotropin (1,500 IU) injection, on the 14-17th day of menstruation collecting oocytes. The difference between protocol I and protocol II was that 0.1 mg the gonadotropin-releasing hormone agonist was injected on day 1 of the menstruation, while the difference between personalized superovulation protocol and protocol II was that oocytes could be collected on the 14-17th day of menstrual cycle according to the length of each monkey. Results: The total number of oocytes harvested using the personalized superovulation protocol was much higher than that using protocol I (p < 0.05), and the proportion of MII-stage oocytes was significantly greater than that from either superovulation protocol I or II (p < 0.001 and p < 0.01 respectively), while the proportion of immature oocytes at the germinal vesicle was less than that from superovulation protocol I (p < 0.05). Conclusions: The personalized superovulation protocol could increase the rate of MII-stage oocytes acquired, and successfully develop into embryos after intracytoplasmic sperm injection, and eventually generated fetus.

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과제정보

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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