Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Analysis of the transcripts encoding for antigenic proteins of bovine gammaherpesvirus 4

  • Romeo, Florencia (Agencia Nacional de Promoción Científica y Tecnologica) ;
  • Spetter, Maximiliano J. (Consejo Nacional de Investigaciones Científicas y Tecnicas) ;
  • Moran, Pedro (Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires) ;
  • Pereyra, Susana (Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Balcarce) ;
  • Odeon, Anselmo (Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Balcarce) ;
  • Perez, Sandra E. (Consejo Nacional de Investigaciones Científicas y Tecnicas) ;
  • Verna, Andrea E. (Consejo Nacional de Investigaciones Científicas y Tecnicas)
  • Received : 2019.07.12
  • Accepted : 2019.10.21
  • Published : 2020.01.31
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Abstract

The major glycoproteins of bovine gammaherpesvirus 4 (BoHV-4) are gB, gH, gM, gL, and gp180 with gB, gH, and gp180 being the most glycosylated. These glycoproteins participate in cell binding while some act as neutralization targets. Glycosylation of these envelope proteins may be involved in virion protection against neutralization by antibodies. In infected cattle, BoHV-4 induces an immune response characterized by low neutralizing antibody levels or an absence of such antibodies. Therefore, virus seroneutralization in vitro cannot always be easily demonstrated. The aim of this study was to evaluate the neutralizing capacity of 2 Argentine BoHV-4 strains and to associate those findings with the gene expression profiles of the major envelope glycoproteins. Expression of genes coding for the envelope glycoproteins occurred earlier in cells infected with isolate 10/154 than in cells infected with strain 07/435, demonstrating a distinct difference between the strains. Differences in serological response can be attributed to differences in the expression of antigenic proteins or to post-translational modifications that mask neutralizing epitopes. Strain 07/435 induced significantly high titers of neutralizing antibodies in several animal species in addition to bovines. The most relevant serological differences were observed in adult animals. This is the first comprehensive analysis of the expression kinetics of genes coding for BoHV-4 glycoproteins in 2 Argentine strains (genotypes 1 and 2). The results further elucidate the BoHV-4 life cycle and may also help determine the genetic variability of the strains circulating in Argentina.

Keywords

Acknowledgement

All authors agree to the conditions outlined in the copyright assignment form included. This work was supported by Agencia Nacional de Tecnologia (FONCyT) through PICT 2015-2263.

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