The Journal of Korean Academy of Prosthodontics (대한치과보철학회지)

The Korean Academy of Prosthodonitics (대한치과보철학회)
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The biofilm removal effect of MnO2-diatom microbubbler from the dental prosthetic surfaces: In vitro study

치과 보철 재료 표면에서 MnO2-diatom microbubbler의 세균막 제거 효과 연구: In vitro study

  • Lee, Eun-Hyuk (Department of Prosthodontics, School of Dentistry, Seoul National University) ;
  • Seo, Yongbeom (Department of Chemical and Biomolecular Engineering, University of Illinois) ;
  • Kwon, Ho-Bum (Department of Prosthodontics, School of Dentistry, Seoul National University) ;
  • Yim, Young-Jun (Department of Prosthodontics, School of Dentistry, Seoul National University) ;
  • Kong, Hyunjoon (Department of Chemical and Biomolecular Engineering, University of Illinois) ;
  • Kim, Myung-Joo (Department of Prosthodontics, School of Dentistry, Seoul National University)
  • 이은혁 (서울대학교 치의학대학원 치과보철학교실) ;
  • 서용범 (일리노이대학 화학 및 생물분자공학과) ;
  • 권호범 (서울대학교 치의학대학원 치과보철학교실) ;
  • 임영준 (서울대학교 치의학대학원 치과보철학교실) ;
  • 공현준 (일리노이대학 화학 및 생물분자공학과) ;
  • 김명주 (서울대학교 치의학대학원 치과보철학교실)
  • Received : 2019.09.16
  • Accepted : 2019.10.07
  • Published : 2020.01.31
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Abstract

Purpose: The aim of this study is to evaluate the effectiveness of MnO2-diatom microbubbler (DM) on the surface of prosthetic materials as a mouthwash by comparing the biofilm removal effect with those previously used as a mouthwash in dental clinic. Materials and methods: DM was fabricated by doping manganese dioxide nanosheets to the diatom cylinder surface. Scanning electron microscopy (SEM) was used to observe the morphology of DM and to analyze the composition of doped MnO2. Stereomicroscope was used to observe the reaction of DM in 3% hydrogen peroxide. Non-precious metal alloys, zirconia and resin specimens were prepared to evaluate the effect of biofilm removal on the surface of prosthetic materials. And then Streptococcus mutans and Porphyromonas gingivalis biofilms were formed on the specimens. When 3% hydrogen peroxide solution and DM were treated on the biofilms, the decontamination effect was compared with chlorhexidine gluconate and 3% hydrogen peroxide solution by crystal violet staining. Results: Manganese dioxide was found on the surface of the diatom cylinder, and it was found to produce bubble of oxygen gas when added to 3% hydrogen peroxide. For all materials used in the experiments, biofilms of the DM-treated groups got effectively removed compared to the groups used with chlorhexidine gluconate or 3% hydrogen peroxide alone. Conclusion: MnO2-diatom microbubbler can remove bacterial membranes on the surface of prosthetic materials more effectively than conventional mouthwashes.

목적: 본 연구의 목적은 보철 재료 표면에서 MnO2-diatom microbubbler (DM)의 세균막 제거 효과를 기존에 치과 임상에 구강세정제로 사용되고 있는 성분들과 비교하여 이 재료가 구강세정제로 사용될 수 있는 가능성을 평가하는 것이다. 재료 및 방법: 이산화망간 나노 시트가 도핑된 DM을 만들었고, 주사전자현미경(SEM)을 이용하여 형태에 대한 관찰 및 도핑된 MnO2의 성분 분석을 시행하였다. 3% 과산화수소수에서 DM의 반응을 시간에 따라 관찰하기 위해 실체 현미경을 이용하였다. 보철 재료 표면의 세균막 제거 효과를 평가하기 위해 비귀금속 합금, 지르코니아, 레진 시편을 제작하였고 치아우식의 원인균이며 호기성 세균인 Streptococcus mutans와 치주질환의 원인균이며 혐기성 세균인 Porphyromonas gingivalis 세균막을 각각 형성하였다. 형성된 세균막에 3% 과산화수소수와 DM을 처리하였을 때 세균막 제거 효과를 클로르헥시딘 글루코네이트와 3% 과산화수소수의 경우와 crystal violet 염색 실험을 통해 비교 평가하였다. 결과: 속이 빈 원통 형태의 규조류에 이산화망간 성분이 발견되었고, 3% 과산화수소수에서 기체를 만들어내는 것을 확인할 수 있었다. 실험에 이용된 모든 재료에서 DM을 처리한 군이 클로르헥시딘 글루코네이트나 3% 과산화수소수 단독으로 사용한 군에 비해 통계적으로 유의하게 세균막을 효과적으로 제거하였다. 결론: MnO2-diatom microbubbler는 보철 재료 표면의 세균막을 기존의 구강세정제 성분에 비해 더 효과적으로 제거할 수 있다.

Keywords

References

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