The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Protective Effect of Corni Fructus Extracts on MIA-induced Animal Model of Osteoarthritis: Effect of Corni Fructus Extracts on OA

MIA로 유발된 골관절염 동물모델에서 산수유 추출물의 골관절염 개선 효과

  • Baek, Kyungmin (Dept. of Cardiovascular and Neurologic Diseases of Korean Internal Medicine, Daegu Korean Medicine Hospital of Daegu Haany University) ;
  • An, Yu-min (Dept. of Cardiovascular and Neurologic Diseases of Korean Internal Medicine, Daegu Korean Medicine Hospital of Daegu Haany University) ;
  • Shin, Mi-Rae (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Min Ju (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Jin A (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Ro, Seong-Soo (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
  • 백경민 (대구한의대학교부속한방병원 심계내과학교실) ;
  • 안유민 (대구한의대학교부속한방병원 심계내과학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김민주 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2020.01.06
  • Accepted : 2020.02.07
  • Published : 2020.04.20
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Abstract

Objectives: Osteoarthritis (OA) is a chronic and degenerative joint disease characterized by progressive degeneration of articular cartilage. Inflammation is a recognized and important factor of OA progression. The present study was designed to investigate the protective effect of Corni Fructus water extract (CFW) on a monosodium iodoacetate (MIA)-induced rat model of OA. Methods: Osteoarthritis was induced by injection of MIA (50 µL; 80 mg/mL) into the knee joint cavity of rats. After an adaptation period for seven days, the rats were divided into 4 groups (n=8/group): normal, control, indomethacin-treated (5 mg/kg), and CFW-treated (200 mg/kg) groups. The rats were treated orally for 14 days. Pain was evaluated by determining hind paw weight distribution. For biochemical analyses, we measured the changes in reactive oxygen species (ROS) and peroxynitrite (ONOO-) in the knee joint. The presence of anti-oxidant proteins and inflammatory proteins was determined by western blotting. Results: The administration of CFW significantly improved the hind paw weight distribution. The ROS and ONOO- levels of knee joint were significantly decreased in the CFW group. CFW inhibited the production of inflammatory mediators, such as COX-2, and inflammatory cytokines, including IL-6 and IL-1β, via the NF-κB signaling pathway. The expression of anti-oxidant enzymes, such as catalase and GPx-1/2 also increased significantly. Conclusions: The findings indicate that CFW has a therapeutic and protective effect on OA by suppression of inflammation. Therefore, CFW could represent a potential and effective candidate for OA treatment.

Keywords

References

  1. The Society of Korean Medicine Rehabilitation. Oriental rehabilitation medicine. 2nd rev. Seoul: Koonja; 2005, p. 84, 97.
  2. Park JS, Lee HJ, Lee DY, Jo HS, Jeong JH, Kim DH, et al. Chondroprotective effects of wogonin in experimental models of osteoarthritis in vitro and in vivo. Biomol Ther 2015;23(5):442-8. https://doi.org/10.4062/biomolther.2015.045
  3. Braza-Boils A, Ferrandiz ML, Terencio MC, Alcaraz MJ. Analysis of early biochemical markers and regulation by tin protoporphyrin IX in a model of spontaneous osteoarthritis. Exp Gerontol 2012;47(5):406-9. https://doi.org/10.1016/j.exger.2012.03.005
  4. Choi DJ, Choi SI, Choi BR, Lee YS, Lee DY, Kim GS. Cartilage protective and anti-analgesic effects of ALM16 on monosodium iodoacetate induced osteoarthritis in rats. BMC Complement Altern Med 2019;19(1):325. https://doi.org/10.1186/s12906-019-2746-7
  5. Greene MA, Loeser RF. Aging-related inflammation in osteoarthritis. Osteoarthritis Cartilage 2015;23(11):1966-71. https://doi.org/10.1016/j.joca.2015.01.008
  6. Kim YJ. Effects of Curcuma longa Rhizoma on MIA-induced Osteoarthritis in Rat Model. J Korean Med 2019;40(3):35-58. https://doi.org/10.13048/jkm.19026
  7. Park S, Kim YS, Lee D, Kwon Y, Park J, Lee SY, et al. Efficacy and safety of HT008 and glucosamine sulfate in the treatment of knee osteoarthritis: a randomized double-blind trial. Kor J Herbology 2014;29(4):45-52. https://doi.org/10.6116/KJH.2014.29.4.45
  8. Jhun HJ, Ahn K, Lee SC. Estimation of the prevalence of asteoarthritis in Korean adults based on the data from the fourth Korea national health and nutrition examination survey. Anesth Pain Med 2010;5(3):201-6.
  9. Yun JH. Osteoarthritis update. J Korean Med 2012;82(2):170-4. https://doi.org/10.3904/kjm.2012.82.2.170
  10. Liu CC. Anti-Oxidants and Anti-inflammation Effects of Geongangbuja-tang. Daejoen: Graduate school, Daejoen Univ; 2011.
  11. Choi WJ. The antioxidative and anti-inflammatory effects of lophatheri extracts for herbal-acupuncture via reducing iNOS synthesis induced by LPS in RAW 264.7 cell. Iksan: Graduated school, Wonkwong Univ; 2010.
  12. Park SD. Effect of Cervi Pantotrichum Cornu herbal acupuncture on protease activities anti-oxidant in rheumatoid arthritis rats. The Journal of Korean Acupuncture and Moxibustion Society 2002;19(2):51-64.
  13. An SS, Heo DS. Effects of Kyejiinsam-tang in MIA-induced Osteoarthritis Rats. Journal of Korean Med 2013;34(3):69-85. https://doi.org/10.13048/jkm.13013
  14. Kim YJ. Effects of Curcuma longa Rhizoma on MIA-induced Osteoarthritis in Rat Model. J Korean Med 2019;40(3):35-58. https://doi.org/10.13048/jkm.19026
  15. Kim EN, Roh SS, Jeong GS. Inhibitory Effect of Sparassis crispa(Wulf.) Extract on Monosodium Iodacetate Induced Osteoarthritis. Journal of Korean Pharmacogn 2018;49(3):262-9.
  16. Kim IL, Kim HC, Guk YB, Park SJ, Park YK, Park JH, et al. Bonchohak. 2rd rev. Seoul: Yunglimsa; 2011, p. 688-9.
  17. Sung YH, Chang HK, Kim SE, Kim YM, Seo JH, Shin MC, et al. Anti-inflammatory and analgesic effects of the aqueous extract of corni fructus in murine RAW 264.7 macrophage cells. J Med Food 2009;12(4):788-95. https://doi.org/10.1089/jmf.2008.1011
  18. Kang DG, Choi DH, Lee JK, Lee YJ, Moon MK, Yang SN, et al. Endothelial NO/cGMPdependent vascular relaxation of cornuside isolated from the fruit of Cornus officinalis. Planta Med 2007;73(14):1436-40. https://doi.org/10.1055/s-2007-990243
  19. Kim KS, Lee BR, Yang GY, An TW, Yim YK. Effects of the Herbal-Acupuncture with Corni Fructus Extract at Eumgok on Osteoporosis in Ovariectomized Mice. Korean Journal of Acupuncture 2010;27(1):63-85.
  20. Shin JH, Cha GY, Kim HJ, Hwang JH, Han KH, Seo HJ, et al. Exmination of Anti-Obesity Effect of RegionalSpecial Natural Products of Anthrisci radix, Psoraleaesemen, Siegesbeckiae herba and Corni fructus. KSBB Journal 2009;24(6):549-55.
  21. Park EB, Kim HS, Shin SY, Ji IA, Kim JH, Kim SG, et al. Antioxidative activity of Cornus Officaianalis extract obtained by four different extraction techniques. Journal of life Science 2012;22(11):1507-14. https://doi.org/10.5352/JLS.2012.22.11.1507
  22. Kim OK. Antidiabetic and antioxidative effects of corni fructus in streptozotocin-induced diabetic rats. J Korean Oil Chemists' Soc 2005;22(2):157-67.
  23. Yamabe N, Noh JS, Park CH, Kang KS, Shibahara N, Tanaka T, et al. Evaluation of loganin, iridoid glycoside from Coni Fructus on hepatic and renal glucoilipotoxicity and inflammation in typ 2 diabetic db/db mice. EUR J Pharmacol 2010 Dec 1;648(1-3):179-87. https://doi.org/10.1016/j.ejphar.2010.08.044
  24. Kwon SH, Kim HC, Lee SY, Jang CG. Loganin improves learning and momory impairments induced by scopolamine in mic. EUR J Pharmacol 2009;619(1-3):44-9. https://doi.org/10.1016/j.ejphar.2009.06.062
  25. Korean Acupuncture & Moxibustion Society. The Acupuncture and Moxibustion Medicine. Paju: Jibmoondang; 2012.
  26. Ali SF, LeBel CP, Bondy SC. Reactive oxygen species formation as a biomarker of methylmercury and trimethyltin neurotoxicity. Neurotoxicology 1992;13(3):637-48.
  27. Kooy NW, Royall JA, Ischiropoulos H, Beckman JS. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radical Biology and Medicine 1994;16(2):149-56. https://doi.org/10.1016/0891-5849(94)90138-4
  28. Korean Acupuncture & Moxibustion Medicine Society. Acupuncture Medicine. Seoul: Hanmibook; 2016, p. 537-68.
  29. Johnston SA. Joint Anatomy, Physiology and Pathobiology. Vet Clin North Am Samll Anim Pract 1997;27(4):699-723. https://doi.org/10.1016/S0195-5616(97)50076-3
  30. Elisha IL, Dzoyem JP, McGaw LJ, Botha FS, Eloff JN. The anti-arthritic, anti-inflammatory, antioxidant activity and relationships with total phenolics and total flavonoids of nine South African plants used traditionally to treat arthritis. BMC Complement Altern Med 2016;16:307. https://doi.org/10.1186/s12906-016-1301-z
  31. Gupta RK, Patel AK, Shah N, Chaudhary AK, Jha UK, Yadav UC, et al. Oxidative stress and antioxidants in disease and cancer: a review. Asian Pac J Cancer Prev 2014;15(11):4405-9. https://doi.org/10.7314/APJCP.2014.15.11.4405
  32. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39(1):44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  33. Spector A. Oxidative Stress and Disease. J Ocul Pharmacol Ther 2000;16(2):193-201. https://doi.org/10.1089/jop.2000.16.193
  34. Bedard K, Krause KH. The NOX family of ROS-generating NADPH Oxidases: Physiology and Pathophysiology. Physiol Rev 2007;87(1):245-313. https://doi.org/10.1152/physrev.00044.2005
  35. Hann GPMM, PAquay JBG, Korthouwer REM, Bast A. Peroxynitrite scavenging by flavonoids. Biochem Biophy Res Commu 1997;236(3):591-3. https://doi.org/10.1006/bbrc.1997.7016
  36. Wang W, Gao C, Ren L, Luo Y. The Effect of Longwave Ultraviolet Light Radiation on Dendrolimus tabulaeformis Antioxidant and Detoxifying Enzymes. Insects 2019;11(1). pii: E1.
  37. Van Raamsdonk JM, Hekimi S. Superoxide dismutase is dispensable for normal animal lifespan. Proc Natl Acad Sci USA 2012;109(15):5785-90. https://doi.org/10.1073/pnas.1116158109
  38. Preiser JC. Oxidative stress. JPEN J Parenter Enteral Nutr 2012;36(2):147-54. https://doi.org/10.1177/0148607111434963
  39. Park SH, Lee HJ, Ryu JH, Lee SY, Shin HD, Hong JH, et al. Effects of Silibinin and resveratol on degradation of $I{\kappa}B$ and translocation of $NF-{\kappa}B$ p65 induced by tumor necrosis $factor-{\alpha}$ in cultured airway epithelial cells. Yakhak Hoji 2014;58(1):1-6.
  40. Lee SM, Kim YK, Hwang YH, Lee CH, Lee HS, Lee CT, et al. Role of $PI3I{\kappa}/Akt$ pathway in the activation of $II{\kappa}B/NF-{\kappa}B$ pathway in lung epithelial cells. Tuberculosis and Respiratory Diseases 2003;54(5):551-62. https://doi.org/10.4046/trd.2003.54.5.551