Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Effects of in vitro Culture Period of Reconstructed Embryos and Genetic Background of Feeder Cells on Establishment of Embryonic Stem Cells Derived from Somatic Cell Nuclear Transfer Blastocysts in Pigs

  • Han, Na Rae (Department of Animal Life Science, Kangwon National University) ;
  • Baek, Song (Department of Animal Life Science, Kangwon National University) ;
  • Lee, Yongjin (College of Veterinary Medicine, Kangwon National University) ;
  • Lee, Joohyeong (Institute of Veterinary Medicine, Kangwon National University) ;
  • Yun, Jung Im (KustoGen Inc.) ;
  • Lee, Eunsong (College of Veterinary Medicine, Kangwon National University) ;
  • Lee, Seung Tae (Department of Animal Life Science, Kangwon National University)
  • Received : 2020.03.13
  • Accepted : 2020.03.25
  • Published : 2020.03.31
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Abstract

The establishment of porcine embryonic stem cells (ESCs) from porcine somatic cell nuclear transfer (SCNT) blastocysts is influenced by in vitro culture day of porcine reconstructed embryo and feeder cell type. Therefore, the objective of the present study was to determine the optimal in vitro culture period for reconstructed porcine SCNT embryos and mouse embryonic fibroblast (MEF) feeder cell type for enhancing colony formation efficiency from the inner cell mass (ICM) of porcine SCNT blastocysts and their outgrowth. As the results, porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days showed significantly increased efficiency in the formation of colonies, compared to those for 7 days. Moreover, MEF feeder cells derived from outbred ICR mice showed numerically the highest efficiency of colony formation in blastocysts produced through in vitro culture of porcine SCNT embryos for 8 days and porcine ESCs with typical ESC morphology were maintained more successfully over Passage 2 on outbred ICR mice-derived MEF feeder cells than on MEF feeder cells derived from inbred C57BL/6 and hybrid B6CBAF1 mice. Overall, the harmonization of porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days and MEF feeder cells derived from outbred ICR mice will greatly contribute to the successful establishment of ESCs derived from porcine SCNT blastocysts.

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References

  1. Baek S, Han NR, Yun JI, Hwang JY, Kim M, Park CK, Lee E, Lee ST. 2017. Effects of culture dimensions on maintenance of porcine inner cell mass-derived cell self-renewal. Mol. Cells 40:117-122. https://doi.org/10.14348/molcells.2017.2223
  2. Brevini T, Pennarossa G, Maffei S, Gandolfi F. 2012. Pluripotency network in porcine embryos and derived cell lines. Reprod. Domest. Anim. 47 Suppl 4:86-91. https://doi.org/10.1111/j.1439-0531.2012.02060.x
  3. Brevini TA, Antonini S, Cillo F, Crestan M, Gandolfi F. 2007. Porcine embryonic stem cells: facts, challenges and hopes. Theriogenology 68 Suppl 1:S206-S213. https://doi.org/10.1016/j.theriogenology.2007.05.043
  4. Cha HJ, Yun JI, Han NR, Kim HY, Baek S, Lee SH, Lee J, Lee E, Park CK, Lee ST. 2018. Generation of embryonic stem-like cells from in vivo-derived porcine blastocysts at a low concentration of basic fibroblast growth factor. Reprod. Domest. Anim. 53:176-185. https://doi.org/10.1111/rda.13088
  5. Chen LR, Shiue YL, Bertolini L, Medrano JF, BonDurant RH, Anderson GB. 1999. Establishment of pluripotent cell lines from porcine preimplantation embryos. Theriogenology 52:195-212. https://doi.org/10.1016/S0093-691X(99)00122-3
  6. Choi KH, Lee DK, Kim SW, Woo SH, Kim DY, Lee CK. 2019. Chemically defined media can maintain pig pluripotency network in vitro. Stem Cell Reports 13:221-234. https://doi.org/10.1016/j.stemcr.2019.05.028
  7. Guo R, Ye X, Yang J, Zhou Z, Tian C, Wang H, Wang H, Fu H, Liu C, Zeng M, Yang J, Liu L. 2018. Feeders facilitate telomere maintenance and chromosomal stability of embryonic stem cells. Nat. Commun. 9:2620. https://doi.org/10.1038/s41467-018-05038-2
  8. Han NR, Baek S, Lee Y, Lee J, Yun JI, Lee E, Lee ST. 2019. Establishment of in-vitro culture system for enhancing production of somatic cell nuclear transfer (SCNT) blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts in pigs. J. Anim. Reprod. Biotechnol. 34:130-138. https://doi.org/10.12750/JARB.34.2.130
  9. Haraguchi S, Kikuchi K, Nakai M, Tokunaga T. 2012. Establishment of self-renewing porcine embryonic stem cell-like cells by signal inhibition. J. Reprod. Dev. 58:707-716. https://doi.org/10.1262/jrd.2012-008
  10. Hou DR, Jin Y, Nie XW, Zhang ML, Ta N, Zhao LH, Yang N, Chen Y, Wu ZQ, Jiang HB, Li YR, Sun QY, Dai YF, Li RF. 2016. Derivation of porcine embryonic stem-like cells from i n vitro-produced blastocyst-stage embryos. Sci. Rep. 6:25838. https://doi.org/10.1038/srep25838
  11. Kim E and Hyun SH. 2016. Apoptosis in porcine pluripotent cells: from ICM to iPSCs. Int. J. Mol. Sci. 17:E1533.
  12. Lee J, Lee Y, Jung HH, Lee ST, Lee GS, Lee E. 2018. Effect of glycine and various osmolarities of culture medium on in vitro development of parthenogenesis and somatic cell nuclear transfer embryos in pigs. J. Emb. Trans. 33:221-228. https://doi.org/10.12750/JET.2018.33.4.221
  13. Li M, Ma W, Hou Y, Sun XF, Sun QY, Wang WH. 2004. Improved isolation and culture of embryonic stem cells from Chinese miniature pig. J. Reprod. Dev. 50:237-244. https://doi.org/10.1262/jrd.50.237
  14. Li P, Wang S, Zhan L, He X, Chi G, Lv S, Xu Z, Xia Y, Teng S, Li L, Li Y. 2017. Efficient feeder cells preparation system for largescale preparation and application of induced pluripotent stem cells. Sci. Rep. 7:12266. https://doi.org/10.1038/s41598-017-10428-5
  15. Llames S, Garcia-Perez E, Meana A, Larcher F, del Rio M. 2015. Feeder layer cell actions and applications. Tissue. Eng. Part B Rev. 21:345-353. https://doi.org/10.1089/ten.teb.2014.0547
  16. Nakamura Y, Tajima S, Kikuchi K. 2017. The quality after culture in vitro or in vivo of porcine oocytes matured and fertilized in vitro and their ability to develop to term. Anim. Sci. J. 88:1916-1924. https://doi.org/10.1111/asj.12855
  17. Nii T, Marumoto T, Kawano H, Yamaguchi S, Liao J, Okada M, Sasaki E, Miura Y, Tani K. 2014. Analysis of essential pathways for self-renewal in common marmoset embryonic stem cells. FEBS Open Bio 4:213-219. https://doi.org/10.1016/j.fob.2014.02.007
  18. Park YG, Lee SE, Kim EY, Hyun H, Shin MY, Son YJ, Kim SY, Park SP. 2015. Effects of feeder cell types on culture of mouse embryonic stem cell in vitro. Dev. Reprod. 19:119-126. https://doi.org/10.12717/DR.2015.19.3.119
  19. Siriboon C, Lin YH, Kere M, Chen CD, Chen LR, Chen CH, Tu CF, Lo NW, Ju JC. 2015. Putative porcine embryonic stem cell lines derived from aggregated four-celled cloned embryos produced by oocyte bisection cloning. PLoS One 10:e0118165. https://doi.org/10.1371/journal.pone.0118165
  20. Son HY, Kim JE, Lee SG, Kim HS, Lee E, Park JK, Ka H, Kim HJ, Lee CK. 2009. Efficient derivation and long term maintenance of pluripotent porcine embryonic stem-like cells. Asian-Aust. J. Anim. Sci. 22:26-34. https://doi.org/10.5713/ajas.2009.80343
  21. Talbot NC, Sparks WO, Powell AM, Kahl S, Caperna TJ. 2012. Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells. In Vitro Cell. Dev. Biol. Anim. 48:1-11.
  22. Tan G, Ren L, Huang Y, Tang X, Zhou Y, Zhou Y, Li D, Song H, Ouyang H, Pang D. 2012. Isolation and culture of embryonic stem-like cells from pig nuclear transfer blastocysts of different days. Zygote 20:347-352. https://doi.org/10.1017/s096719941100030x
  23. Vackova I, Ungrova A, Lopes F. 2007. Putative embryonic stem cell lines from pig embryos. J. Reprod. Dev. 53:1137-1149. https://doi.org/10.1262/jrd.19108
  24. Wianny F, Perreau C, Hochereau de Reviers MT. 1997. Proliferation and differentiation of porcine inner cell mass and epiblast in vitro. Biol. Reprod. 57:756-764. https://doi.org/10.1095/biolreprod57.4.756
  25. Yamanaka K, Sugimura S, Wakai T, Kawahara M, Sato E. 2009. Difference in sensitivity to culture condition between in vitro fertilized and somatic cell nuclear transfer embryos in pigs. J. Reprod. Dev. 55:299-304. https://doi.org/10.1262/jrd.20174
  26. Yang H, Qiu Y, Zeng X, Ding Y, Zeng J, Lu K, Li D. 2016. Effect of a feeder layer composed of mouse embryonic and human foreskin fibroblasts on the proliferation of human embryonic stem cells. Exp. Ther. Med. 11:2321-2328. https://doi.org/10.3892/etm.2016.3204
  27. Zhang M, Wang C, Jiang H, Liu M, Yang N, Zhao L, Hou D, Jin Y, Chen Q, Chen Y, Wang J, Dai Y, Li R. 2019. Derivation of novel naive-like porcine embryonic stem cells by a reprogramming factor-assisted strategy. FASEB J. 33:9350-9361. https://doi.org/10.1096/fj.201802809r