DOI QR코드

DOI QR Code

A comparative study on the phytochemical and anti-oxidant activity differences in HongJam prepared with various silkworm varieties

  • Nguyen, Phuong (Ilsong institute of life sciences, Hallym University) ;
  • Kim, Seong-Wan (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jo, You-Young (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Beteta, Sofia Pallais (Ilsong institute of life sciences, Hallym University) ;
  • Kang, Sang Kook (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Soo Bae (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, NamSook (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kweon, Hae Yong (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Kee-Young (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Koh, Young Ho (Ilsong institute of life sciences, Hallym University)
  • 투고 : 2020.09.27
  • 심사 : 2020.10.29
  • 발행 : 2020.12.31

초록

The aim of this study was to elucidate the difference in the amount of phytochemicals and anti-oxidant activities of the freeze-dried 5th instar 3rd day silkworm powder (FDSP) and 7 HongJams (HJs). Three primary colors of light of 8 silkworm powders revealed why each sample had unique color. The visual and absorption spectrum analyses of 80% methanol (MeOH) extracts of 8 silkworm powders revealed that white jade (WJ)-FDSP extracts showing strong forest-green colors had different absorption spectrum from 7 HJs. Among 7 HJs, the absorption spectrum of light green silkworm (LGS)-HJ extract was different from those of other HJ extracts. The amounts of total flavonoids and phenolic compounds of WJ-FDSP were 423.0±9.21% and 252.3±12.86%, respectively, more than those of WJ-HJ. Among 7 HJs, the amounts of total flavonoids and phenolic compounds in LGS-and red silkorm (RS)-HJ extracts were significantly more than those of WJ-HJ extract. Consistent with the amounts of phytochemicals, radical scavenging activity and reducing ability of WJ-FDSP extract were significantly higher that other HJ extracts. Among 7 silkworm powder extracts, LGS-HJ extract showed significantly higher radical scavenging activity and reducing ability than those of WJ-HJ extract. But, RS-HJ extract only showed significantly higher radical scavenging activity than that of WJ-HJ extract. Taken together, the amounts of phytochemicals in 8 silkworm powders contributed on their colors and anti-oxidant activities.

키워드

참고문헌

  1. Bae S-M, Jo Y-Y, Lee K-G, Kim H-B, Kweon H (2016) Antioxidant activity of silkworm powder treated with protease. Int J Indust Entomol 33,78-84 https://doi.org/10.7852/ijie.2016.33.2.78
  2. Choi BH, Ji SD, Jeong JH, Kim KY, Koh YH (2017a) Quantification and comparison of functional phytochemicals i steamed and freezedried mature silkworm powders and freeze-dried mulberry leaves. Int J Indust Entomol 35, 89-96. https://doi.org/10.7852/IJIE.2017.35.2.89
  3. Choi BH, Ji SD, Son JG, Nguyen P, Kim KY, Park YH, et al. (2017b) Phytochemicals and silk proteins in mature silkworm powders responsible for extended life expectancy and enhanced resistances to Parkinson's disease. J Asia-Pac Entomol 20, 1425-1433. https://doi.org/10.1016/j.aspen.2017.10.011
  4. Do S, Park J, Nam H, Kim J, Lee J, Oh Y, et al. (2012) Silk fibroin hydrolysate exerts an anti-diabetic effect by increasing pancreatic β cell mass in C57BL/KsJ-db/db mice. J Vet Sci 13,339-344 https://doi.org/10.4142/jvs.2012.13.4.339
  5. Ji SD, Kim NS, Kweon HY, Choi BH, Kim KY, Koh YH (2016a) Nutrition composition differences among steamed freeze-dried mature silkworm larval powders made from 3 Bombyx mori varieties weaving different colored cocoons. Int J Indust Entomol 33, 6-14. https://doi.org/10.7852/ijie.2016.33.1.6
  6. Ji SD, Kim NS, Lee JY, Kim MJ, Kweon H, Sung G, et al. (2015) Development of processing technology for edible mature silkworm. J Seric Entomol Sci 53, 38-43. https://doi.org/10.7852/jses.2015.53.1.38
  7. Ji SD, Son JG, Kim S, Kim NS, Kim KY, Kweon H, Sung GK, et al. (2017) Production techniques to improve the quality of steamed and freeze-dried mature silkworm larval powder Int J Indust Entomol 34, 1-11. https://doi.org/10.7852/ijie.2017.34.1.1
  8. Ji SD, Kim SB, Kim KY, Kim NS, Kim SW, Jo YY, et al. (2019) Contents of nutrients in ultra-fine powders of steamed and lyophilized mature silkworms generated by four silkworm varieties. J Asia-Pac Entomol 22, 969-974. https://doi.org/10.1016/j.aspen.2019.07.009
  9. Kang PD, Lee SU, Jung IY, Shon BH, Kim YS, Kim KY, et al. (2007) Breeding of new silkworm variety golden silk, a yellow cocoon color for spring rearing season. Korean J Seric Sci 49, 14-17
  10. Kang PD, Sung GB, Kim KY, Lee HS, Ki M0J, Ji SD., et al. (2011) Breeding of new silkworm strain yeonnokjam. Int J Indust Entomol 23, 175-178 https://doi.org/10.7852/ijie.2011.23.1.175
  11. Kim DK, Kang YK, Lee MY, Lee KG, Yeo JH, Lee WB, et al. (2005) Neuroprotection and enhancement of learning and memory by BF-7 J Health Sci, 5, 317-324.
  12. Kim HJ, Kim KY, Ji SD, Lee HT (2017) Anti-melanogenic activity of steamed and freeze-dried mature silkworm powder. J Asia-Pac Entomol 20, 1001-1006. https://doi.org/10.1016/j.aspen.2017.07.013
  13. Kim KY, Osabutey AF, Nguyen P, Kim SB, Jo YY, Kweon H, et al. (2019) The experimental evidences of steamed and freeze-dried mature silkworm powder as the calorie restriction mimetics. Int J Indust Entomol 39, 1-8. https://doi.org/10.7852/ijie.2019.39.1.1
  14. Koh YH (2020) The memory enhancement and healthspan extension effects of HongJam The Policy Report of National Institute for Korean Medicine Development 5, 22-33
  15. Lee SP, Hong KW, Sohn KW, Mah YI, Kim KY (1984) Breeding of new silkworm variety "Baegokjam" Research Rep RDA 26, 58-64
  16. Martel J, Ojcius DM, Ko YF, Ke PY, Wu C-Y, Peng HH, et al. (2019) Hormetic effects of phytochemicals on health and longevity. Trends Endrocrinol Meta 30, 335-346. https://doi.org/10.1016/j.tem.2019.04.001
  17. Nguyen P, Kim KY, Kim AY, Kim NS, Kweon HY, Ji SD, et al. (2016) Increased healthspan and resistance to Parkinson's disease in Drosophila by boiled and freeze-dried mature silk worm larval powder. J Asia-Pac Entomol 19, 551-561. https://doi.org/10.1016/j.aspen.2016.05.003
  18. Nguyen P, Kim KY, Kim AY, Choi BH, Osabutey AF, Park YH, et al. (2020) Mature silkworm powders ameliorated scopolamine-induced amnesia by enhancing mitochondrial functions in the brains of mice. J Funct Foods 67, 103886. https://doi.org/10.1016/j.jff.2020.103886
  19. Ryu KS, Ahn MY, Lee HS, Kim I, Kim JW, Kim SH, et al. (2002a) The tonic effect of the extract from male silkworm (Bombyx mori L.) pupae on rats Int J Indust Entomol 5, 123-126
  20. Ryu KS, Lee HS, Chung SH, Kang PD (1997) An activity of lowering blood-glucose levels accoring to preparative conditions of silkworm powder. J Seric Entomol Sci 39, 79-85
  21. Ryu KS, Lee HS, Kim IS (2002b) Effects and mechanisms of silkworm powder as a blood glucose-lowering agent. Int J Indust Entomol 4, 93-100
  22. Ryu KS, Lee HS, Kim KY, Kim MJ, Kang PD, Chun SN, et al. (2012) Anti-diabetic effects of the silkworm (Bombyx mori.) extracts in the db/db mice Planta Med 78, PI458.
  23. Thaipitakwong T, Numhom S, Aramwit P (2018) Mulberry leaves and their potential effects against cardiometabolic risks: a review of chemical compositions, biological properties and clinical efficacy. Pharm Biol 56, 109-118. https://doi.org/10.1080/13880209.2018.1424210
  24. Tsuchida K, Sakudoh T (2015) Recent progress in molecular genetic studies on the carotenoid transport system using cocoon-color mutants of the silkworm. Arch Biochem Biophys 572, 151-157. https://doi.org/10.1016/j.abb.2014.12.029
  25. Yun SM, Cho JM, Hong KS, Lee DY, Ji SD, Son JG, et al. (2017) Gastroprotective effect of mature silkworm, Bombyx mori against ethanol-induced gastric mucosal injuries in rats. J Funct Foods 39, 279-286. https://doi.org/10.1016/j.jff.2017.10.036