Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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The Expression changes of AMPK, ERK-1/2, and p38 protein associated with Exercise in the Mouse hippocampus exposed to Radiofrequency Radiation

전자파(電磁波)에 노출된 생쥐의 해마에서 운동이 AMPK, ERK-1/2, p38 단백 발현 변화에 미치는 생체 영향

  • 이민선 (신한대학교 간호대학) ;
  • 박옥진 (신한대학교 카이로프랙틱학과) ;
  • 김현택 (선문대학교 일반대학원) ;
  • 김명주 (단국대학교 의과대학 해부학교실)
  • Received : 2020.02.06
  • Accepted : 2020.03.20
  • Published : 2020.03.28
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Abstract

To determine the biological effects of exercise on hippocampus in mice brain exposed to radiofrequency radiaton (RF), the expression of AMPKα, p-AMPKα, ERK1/2, p-ERK1/2, p38, and p-p38 protein in the mouse exposed to RF were investigated in the hippocampal tissues, Western blot method was used to compare the protein expression levels for each molecule. Significant increases in protein expression of individual and phosphorylated molecules were observed in the spontaneous exercise group, and the expression of these molecules was notably decreased in the RF exposure and spontaneous exercise group. This study shows that neuroplasticity can be increased by exercise in hippocampus that is responsible for memory, but memory and cognitive function may be affected by exposure to RF. We may expect clinically interesting results on dementia or Alzheimer disease if we proceed further investigation on the effect of RF.

전자파에 노출된 생쥐에서 운동이 해마에 미치는 생체영향을 확인하고자 뇌의 신경세포에서 많이 발현되는 AMPKα, p-AMPKα, ERK1/2, p-ERK1/2, p38, p-p38 단백질 발현의 변화를 해마에서 조사하였다. 10주 동안 생쥐들을 정상군, 운동군, 전자파 노출군, 전자파 노출 및 자발운동군으로 나누어 비교하였다. 생쥐들은 835 MHz의 주파수를 송출하는 Wave Exposer V20을 사용해 전자파에 노출시켰고, 각 분자들에 대한 단백질 발현량의 차이는뇌의 해마를 분리해 Western blot으로 조사했다. 각각의 분자들과 인산화 분자들에서 유의한 단백질 발현의 증가는 운동군에서 있었으며, 전자파 노출 및 운동군에서는 이들 분자들의 발현이 통계적으로 유의한 수준으로 현저히 감소하였다. 따라서, 본 연구는 기억을 담당하는 해마에서 운동에 의해 신경가소성이 증가할 수 있지만, 전자파에 노출되면 기억 및 인지기능이 영향을 받을 수 있어 전자파가 실제 세포수준에서 기억력에 영향을 미칠 수 있음을 보였다. 앞으로 전자파가 치매에 미치는 임상적 영향에 대한 연구를 진행한다면 흥미 있는 결과를 기대할 수 있을 것이다.

Keywords

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